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Recombinant Human VEGF Protein

  • 表达系统: 水稻(Oryza sativa)
  • Accession #: P15692-4
  • 蛋白标签: No tag
  • 生物活性: The ED50 value was lower than 10.0 ng/ml, that is, the corresponding active unit was greater than or equal to 1 x 10^5 units/mg by primary HUVECs cell proliferation method.
  • 内毒素水平: <0.2 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp155965-10μg
10μg 现货 Stock Image
rp155965-50μg
50μg 现货 Stock Image
rp155965-100µg
100μg 现货 Stock Image
rp155965-1mg
1mg 期货 Stock Image

基本描述

产品名称 Recombinant Human VEGF Protein
别名 重组人VEGF蛋白 | 重组人 VEGF 蛋白
英文别名 MVCD1 | VAS | Vascular endothelial growth factor A | Vascular permeability factor | Vasculotropin | VEGF | VEGFA | VEGF-A | VEGFMGC70609 | VPF | VPFvascular endothelial growth factor | OsrhVEGF | Recombinant Human Vascular Endothelial Growth Factor from O
规格或纯度 GMP, 生物活性, ActiBioPure™, High performance, 无动物源, 无载体, ≥95%(SDS-PAGE)
生化机理 Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Post-translational: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
产品介绍

VEGF血管内皮生长因子(Vascular endothelial growth factor),也被称为血管通透因子(Vascular permeability factor,VPF)是一种高度特异性的促血管内皮细胞生长因子,具有促进血管通透性增加、细胞外基质变性、血管内皮细胞迁移、增殖和血管形成等作用。VEGF也被证实对单核细胞和成骨细胞具有趋化作用。
OsrhVEGF是运用基因重组技术,由水稻胚乳细胞表达并通过蛋白提纯工艺而成。

VEGF permeability factor, also known as Vascular permeability factor (VPF), is a highly specific permeability factor for endothelial growth factor. It can promote the increase of vascular permeability, extracellular matrix degeneration, vascular endothelial cell migration, proliferation and angiogenesis. VEGF has also been shown to have chemotaxis on monocytes and osteoblasts.OsrhVEGF is expressed by oryza sativa and purified by protein purification technology.

生物活性 The ED50 value was lower than 10.0 ng/ml, that is, the corresponding active unit was greater than or equal to 1 x 10^5 units/mg by primary HUVECs cell proliferation method.
内毒素水平 <0.2 EU/μg
表达系统 水稻(Oryza sativa)
种属 人(Human)
氨基酸 27-191 aa
序列 APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
纯度 >95%(SDS-PAGE)
蛋白标签 No tag
蛋白长度 Full length protein
无载体 Yes
Accession # P15692-4
来源 重组表达
预测分子量 19.2 kDa
SDS-PAGE 19.2 kDa

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from solution in trehalose, Citrate monohydrate, HSA, pH 6.9
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 100-200 μg/mL. Stock solutions should be stored at 4℃ for 2-7days, stored at -20℃ for long term use, please use as soon as possible to avoid contamination.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at -20℃ for 3 years. Upon delivery aliquot. Avoid freeze/thaw cycle.
CAS编号和信息 127464-60-2
分子类型 蛋白质

图片

Recombinant Human VEGF Protein (rp155965)-Protein Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is typically <10ng/mL.

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批号(Lot Number) 证书类型 货号
ZJ25F0826030 分析证书 rp155965
ZJ25F0826011 分析证书 rp155965
ZJ25F0420963 分析证书 rp155965
ZJ25F0420967 分析证书 rp155965
ZJ25F0420965 分析证书 rp155965
ZJ24F1012597 分析证书 rp155965
ZJ24F1012596 分析证书 rp155965
ZJ24F1012595 分析证书 rp155965
ZJ23F0600447 分析证书 rp155965
ZJ23F0600445 分析证书 rp155965
ZJ23F0600446 分析证书 rp155965

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