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Recombinant Human VEGF 165 Protein

  • 表达系统: CHO
  • Accession #: P15692-4
  • 蛋白标签: C-His
  • 生物活性: Measured in a cell proliferation assay using Human HUVEC cells. The ED50 for this effect is <2 ng/mL. The corresponding specific activity is>1×10^6 IU/mg.
  • 内毒素水平: <10 EU/mg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp166633-10μg
10μg 现货 Stock Image
rp166633-50μg
50μg 现货 Stock Image
rp166633-100μg
100μg 现货 Stock Image
rp166633-1mg
1mg 现货 Stock Image

基本描述

产品名称 Recombinant Human VEGF 165 Protein
别名 重组人VEGF 165蛋白 | 重组人血管内皮生长因子165蛋白
英文别名 Vascular Endothelial Growth Factor | MVCD1 | VAS | vascular endothelial growth factor A | Vascular permeability factor | Vasculotropin | VEGF | VEGFA | VEGF-A | MGC70609 | VPF | VPFvascular endothelial growth factor | L-VEGF | VEGF165
规格或纯度 ActiBioPure™, GMP, 无动物源, 无载体, 生物活性, 高性能, 无菌, ≥98%(SDS-PAGE)
生化机理 N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A.Involved in protecting cells from hypoxia-mediated cell death (By similarity). VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Post-translational: Vascular endothelial growth factor A, long form. Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
产品介绍

VEGF 血管内皮生长因子,是一种有效的生长和血管生成细胞因子。 它刺激内皮细胞的增殖和存活,并促进血管生成和血管通透性。 VEGF 在血管化组织中表达,在正常和病理性血管生成中发挥着重要作用。 大量证据表明 VEGF 参与肿瘤转移和眼内新生血管综合征的诱导。 VEGF 通过三种受体发出信号; fms样酪氨酸激酶(flt-1)、KDR基因产物(KDR的鼠同源物是flk-1基因产物)和flt4基因产物。 重组人 VEGF165 是一种 38.2 kDa、二硫键连接的同二聚蛋白,由两条 165 个氨基酸的多肽链组成。
本产品用重组CHO细胞表达生产,高纯度,无动物源性污染。


生物活性 Measured in a cell proliferation assay using Human HUVEC cells. The ED50 for this effect is <2 ng/mL. The corresponding specific activity is>1×10^6 IU/mg.
内毒素水平 <10 EU/mg
表达系统 CHO
种属 人(Human)
氨基酸 27-191 aa
序列 APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRRHHHHHH
蛋白标签 C-His
无载体 Yes
无动物源 Yes
Accession # P15692-4
来源 重组表达
预测分子量 20 kDa
SDS-PAGE 38.2 kDa

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 10mM PBS, pH 7.4.
复溶 Reconstitute in water to a concentration of 1 mg/mL, for other concentrations, please adjust the concentration of phosphate in the solution by yourself.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at -20℃~-80℃ (2 years). Upon delivery aliquot. Avoid freeze/thaw cycle.
分子类型 蛋白质

图片

Recombinant Human VEGF 165 Protein (rp166633) - Protein Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1.7 ng/mL.

Recombinant Human VEGF 165 Protein (rp166633) - SDS-PAGE
2 μg/lane of Recombinant Human VEGF 165 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 25 kDa.

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批号(Lot Number) 证书类型 货号
ZJ25F0521447 分析证书 rp166633
ZJ25F0521446 分析证书 rp166633
ZJ25F0521445 分析证书 rp166633
ZJ25F0521444 分析证书 rp166633

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