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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| rp166633-10μg |
10μg |
现货 ![]() |
| |
| rp166633-50μg |
50μg |
现货 ![]() |
| |
| rp166633-100μg |
100μg |
现货 ![]() |
| |
| rp166633-1mg |
1mg |
现货 ![]() |
|
| 产品名称 | Recombinant Human VEGF 165 Protein |
|---|---|
| 别名 | 重组人VEGF 165蛋白 | 重组人血管内皮生长因子165蛋白 |
| 英文别名 | Vascular Endothelial Growth Factor | MVCD1 | VAS | vascular endothelial growth factor A | Vascular permeability factor | Vasculotropin | VEGF | VEGFA | VEGF-A | MGC70609 | VPF | VPFvascular endothelial growth factor | L-VEGF | VEGF165 |
| 规格或纯度 | ActiBioPure™, GMP, 无动物源, 无载体, 生物活性, 高性能, 无菌, ≥98%(SDS-PAGE) |
| 生化机理 | N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A.Involved in protecting cells from hypoxia-mediated cell death (By similarity). VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Post-translational: Vascular endothelial growth factor A, long form. Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form. |
| 产品介绍 |
VEGF 血管内皮生长因子,是一种有效的生长和血管生成细胞因子。 它刺激内皮细胞的增殖和存活,并促进血管生成和血管通透性。 VEGF 在血管化组织中表达,在正常和病理性血管生成中发挥着重要作用。 大量证据表明 VEGF 参与肿瘤转移和眼内新生血管综合征的诱导。 VEGF 通过三种受体发出信号; fms样酪氨酸激酶(flt-1)、KDR基因产物(KDR的鼠同源物是flk-1基因产物)和flt4基因产物。 重组人 VEGF165 是一种 38.2 kDa、二硫键连接的同二聚蛋白,由两条 165 个氨基酸的多肽链组成。 |
| 生物活性 | Measured in a cell proliferation assay using Human HUVEC cells. The ED50 for this effect is <2 ng/mL. The corresponding specific activity is>1×10^6 IU/mg. |
| 内毒素水平 | <10 EU/mg |
| 表达系统 | CHO |
| 种属 | 人(Human) |
| 氨基酸 | 27-191 aa |
| 序列 | APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRRHHHHHH |
| 蛋白标签 | C-His |
| 无载体 | Yes |
| 无动物源 | Yes |
| Accession # | P15692-4 |
| 来源 | 重组表达 |
| 预测分子量 | 20 kDa |
| SDS-PAGE | 38.2 kDa |
| 物理形态 | 冻干(Lyophilized) |
|---|---|
| 储存缓冲液 | 10mM PBS, pH 7.4. |
| 复溶 | Reconstitute in water to a concentration of 1 mg/mL, for other concentrations, please adjust the concentration of phosphate in the solution by yourself. |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20℃~-80℃ (2 years). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| 分子类型 | 蛋白质 |
Recombinant Human VEGF 165 Protein (rp166633) - Protein Bioactivity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1.7 ng/mL.
Recombinant Human VEGF 165 Protein (rp166633) - SDS-PAGE
2 μg/lane of Recombinant Human VEGF 165 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 25 kDa.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp166633 | |
| 分析证书 | rp166633 | |
| 分析证书 | rp166633 | |
| 分析证书 | rp166633 |
¥899.90