基本描述

产品名称	Streptavidin protein (FITC)
别名	链霉亲和素蛋白 (FITC)
英文别名	FITC Streptavidin \| Streptavidin-FITC \| FITC-streptavidin conjugate \| SAv-FITC \| Streptavidin Fluorescein Conjugated \| Streptavidin-Fluorescein Isothiocyanate \| SA-FITC \| SA (FITC) \| SA V1 \| SA V2 \| strepavidin \| Streptavidin V1 \| Streptavidin V2
规格或纯度	无叠氮钠, Ex:498nm, Em:517nm, Streptavidin Concentration: 1 mg/mL
产品介绍	背景链霉亲和素（Streptavidin）是由streptomyces avidinii菌分泌的四聚体蛋白，可以高度特异性地和生物素（Biotin）结合，结合常数为10⁻¹⁴ mol/L，一分子 Streptavidin 可以结合 4 个 Biotin 分子，这种结合是目前自然界已知最强的非共价作用。 Streptavidin 和鸡蛋清来源的亲和素（Avidin）相比，两者与 Biotin 结合的亲和力高度相似性，但Streptavidin在中性条件下几乎不带电荷，因此Streptavidin的非特异性结合比Avidin低很多，检测时的非特异性背景也就少很多。Streptavidin 可以用于 Biotin 标记的抗体、核酸、蛋白或其他生物素标记分子的检测。Streptavidin (FITC)产品是用高纯度的 Streptavidin 和高纯度的 FITC 染料共价连接，并去除游离FITC。FITC标记的链霉亲和素是间接法荧光染色中一种非常有用的二抗，可以高亲和性地结合生物素化抗体。FITC由488nm的激光激发，可以在最大波长为520nm处检测到其发射光。推荐工作浓度 Flow cytometry: 1:500-1:5000 IF: 1:50-1:1000 Background Streptavidin is a tetrameric bacterial protein isolated from Streptomyces avidinii providing 4 high-affinity biotin binding sites. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin. With a dissociation constant on the order of ≈10⁻¹⁴ mol/L, the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Unlike egg-white avidin, which has a net positive charge at neutral pH and contains about 7% carbohydrate, streptavidin has almost no net charge at neutral pH, does not contain carbohydrate, and exhibits lower non-specific background. Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules. This FITC-streptavidin conjugate was prepared by highly purified Streptavidin and free FITC was removed. Streptavidin (FITC) is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Excitation at 488nm light leads to a fluorescence emission maximum of 520 nm. Recommended Usage： Every lot of Streptavidin-FITC is tested by flow cytometry using biotinylated primary antibodies. From this testing it is recommended that between 0.02 and 0.25 µg of streptavidin be used per 106 cells in a 100 µl staining volume.
蛋白长度	Full length protein
偶联	FITC
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	517nm
Ex/Em(nm)	Ex:498nm;Em:517nm
来源	天然

储存与运输

应用	Flow Cytometry; IF;
物理形态	液体
储存缓冲液	PBS (pH 7.4) buffer containing 1% BSA and 0.05% ProClin™ 300 as a preservative.
浓度	Streptavidin Concentration: 1 mg/mL
储存温度	2-8°C储存,避免反复冻融
运输条件	冰袋运输
稳定性与储存	Shipped at 4℃. Store at 4°C and protected from prolonged exposure to light. Do not freeze.
分子类型	蛋白质

图片

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF-Streptavidin protein (FITC) (rp155876)
ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF-Streptavidin protein (FITC) (rp155876)
ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .

Flow-Streptavidin protein (FITC) (rp155876)

Flow-Streptavidin protein (FITC) (rp155876)
BALB/c splenocytes stained with Biotin-CD45R antibody, followed by Streptavidin protein (FITC) (rp155876) at 1/2500 dilution.

IF: Streptavidin Protein (FITC) (rp155876)

Streptavidin Protein (FITC) (rp155876) - IF
IF analysis of beta Tubulin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (Biotin) (Ab179002) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Streptavidin Protein (FITC) (rp155876) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

应用

应用名称	稀释比例
Flow Cytometry	1:500-1:5000
IF/ICC	1:50-1:500

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找到9个结果

批号(Lot Number)	证书类型	货号
ZJ24F1216185	分析证书	rp155876
ZJ24F1216186	分析证书	rp155876
ZJ23F0300091	分析证书	rp155876
ZJ23F0300089	分析证书	rp155876
ZJ23F0300090	分析证书	rp155876
ZJ23F1202116	分析证书	rp155876
ZJ23F1202117	分析证书	rp155876
ZJ23F1202118	分析证书	rp155876
ZJ25F0319537	分析证书	rp155876

技术文档和文章

RNA斑点杂交实验方案

Categories: 实验方案（Protocols）

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一抗二抗蛋白质电泳和蛋白质印迹斑点杂交

RNA dot blot protocol

Categories: 实验方案（Protocols）

探索主题:

Protein Electrophoresis & Western Blotting Dot Blotting Primary antibodies Secondary Antibodies

RNA dot blot protocol

Categories: 实验方案（Protocols）

探索主题:

Protein Electrophoresis & Western Blotting Dot Blotting Primary antibodies Secondary Antibodies

如何找到合适的二抗？

Categories: 技术文章

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荧光团直接/间接检测酶标记物

溶液计算器

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复溶计算器

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所需的复溶浓度

货号 (SKU)	包装规格	是否现货
rp155876-0.1ml	0.1ml	现货
rp155876-0.5ml	0.5ml	现货
rp155876-1ml	1ml	现货

Streptavidin protein (FITC)

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器