基本描述

产品名称	Recombinant Mouse FGF-21 Protein
别名	重组小鼠FGF-21蛋白
英文别名	FGF21 \| FGF-21 \| fibroblast growth factor 21 \| FGF 21 \| FGF21_HUMAN \| FGFL \| PRO10196 \| UNQ3115 \| Fgf8c
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥96%(SDS-PAGE&HPLC)
产品介绍	Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family and it is specifically induced by HMGCS2 activity. In mice, brown adipose tissue becomes a source of systemic FGF21 after cold exposure. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression and the activity depends on the presence of KLB. FGF-21, in the presence of β-Klotho as a protein cofactor, signals through the FGFR 1c and 4 receptors. Murine FGF-21 shows limited binding to heparin. In addition, Murine FGF-21 respectively shows 81% and 92% a.a. identity to human and rat FGF-21, and it show activity on human and rat cells. Recombinant Murine FGF21 is a 19.9kDa globular protein containing 182 amino acid residues. Purity >96%(SDS-PAGE, HPLC) Additional sequence information A single non-glycosylated polypeptide chain containing 182 amino acids. This product is for the mature full length protein. The signal peptide is not included. Function Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.
生物活性	Fully biologically active when compared to standard. The ED₅₀ as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/ml, corresponding to a specific activity of >2.0 ×10³IU/mg in the presence of 5 µg/ml of rMuβ-Klotho and 10 μg/ml of heparin.
内毒素水平	<0.1 EU/μg
表达系统	E. coli
种属	小鼠(Mouse)
氨基酸	29-210 aa
序列	AYPIPDSSPLLQFGGQVRQRYLYTDDDQDTEAHLEIREDGTVVGAAHRS PESLLELKALKPGVIQILGVKASRFLCQQPDGALYGSPHFDPEACSFREL LLEDGYNVYQSEAHGLPLRLPQKDSPNQDATSWGPVRFLPMPGLLHEP QDQAGFLPPEPPDVGSSDPLSMVEPLQGRSPSYAS
纯度	＞96%(SDS-PAGE, HPLC)
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	Q9JJN1
来源	重组表达
预测分子量	19.9 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB 500mM NaCl, pH7.4.
复溶	This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 下运输。在 +4°C 下短期储存（1-2 周）。收货后建议分装。长期储存于 -20°C。避免冷冻/解冻循环：100% PBS 从 3 脳 PBS 中 0.2 µm 过滤浓缩溶液冻干而成。本品为活性蛋白，在体内可能引起生物反应，请谨慎处理。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Mouse FGF-21 Protein (rp153970)

Recombinant Mouse FGF-21 Protein (rp153970) - Protein Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 μg/mL, corresponding to a specific activity of > 2.0 X 10³ IU/mg in the presence of 5.0 µg/mL of rMuβ-Klotho and 10.0 μg/mL of heparin.

SDS-PAGE: Recombinant Mouse FGF-21 Protein (rp153970)

Recombinant Mouse FGF-21 Protein (rp153970) - SDS-PAGE
3 μg/lane of Recombinant Mouse FGF-21 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 21.1 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0420958	分析证书	rp153970
ZJ23F1101656	分析证书	rp153970
ZJ23F1101655	分析证书	rp153970

技术文档和文章

阿拉丁®靶点蛋白

Categories: 技术文章

探索主题:

药物开发信号通路细胞因子靶点蛋白药物靶点受体免疫检查点细胞表面标记物

细胞培养通用指南

Categories: 实验方案（Protocols）

探索主题:

培养基细胞培养贴壁细胞悬浮细胞生长因子黏附细胞冷冻细胞传代

成纤维细胞生长因子家族（FGF）

Categories: 技术文章

探索主题:

成纤维细胞生长因子家族(FGF) FGF蛋白 FGF抗体细胞因子和生长因子 FGF抑制剂

哺乳动物细胞冷冻保存方案

Categories: 实验方案（Protocols）

探索主题:

胰蛋白酶细胞冷冻哺乳动物细胞系细胞解冻二甲基亚砜 DMSO 冻存管

Mammalian Cell Cryopreservation Protocol

Categories: 实验方案（Protocols）

探索主题:

Trypsin cell freezing DMSO Mammalian Cell line Cell thawing Dimethyl sulfoxide Cryopreservation tube

Mammalian Cell Cryopreservation Protocol

Categories: 实验方案（Protocols）

探索主题:

Trypsin cell freezing DMSO Mammalian Cell line Cell thawing Dimethyl sulfoxide Cryopreservation tube

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

=

浓度

x

体积

x

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

x

体积 1 (V1)

=

浓度 2 (C2)

x

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

=

质量（小瓶）

÷

所需的复溶浓度

货号 (SKU)	包装规格	是否现货
rp153970-5μg	5μg	现货
rp153970-50μg	50μg	现货
rp153970-1mg	1mg	期货
rp153970-500μg	500μg	期货

Recombinant Mouse FGF-21 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器