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Recombinant Human Trefoil Factor 3 Protein

  • 表达系统: E. coli
  • Accession #: Q07654
  • 蛋白标签: No tag
  • 生物活性: Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg.
  • 内毒素水平: <1.0 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp152656-10μg
10μg 现货 Stock Image
rp152656-20μg
20μg 期货 Stock Image
rp152656-50μg
50μg 现货 Stock Image
rp152656-100μg
100μg 现货 Stock Image
rp152656-1mg
1mg 期货 Stock Image

基本描述

产品名称 Recombinant Human Trefoil Factor 3 Protein
别名 重组人 Trefoil Factor 3 蛋白 | 重组人三叶因子 3 蛋白
英文别名 hITF | hP1.B | Intestinal trefoil factor | ITF | mITF | OTTMUSP00000021729 | P1B | Polypeptide P1.B | TFF3 | TFF3_HUMAN | TFI | TREFOIL | Trefoil factor (intestinal) | Trefoil factor 3 UTER_HUMAN | Uteroglobin | hITF | hP1.B | Intestinal trefoil factor |
规格或纯度 ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
产品介绍


Purity

>97% by SDS-PAGE  and HPLC analyses.


Function

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

生物活性 Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg.
内毒素水平 <1.0 EU/μg
表达系统 E. coli
种属 人(Human)
氨基酸 22-80 aa
序列 EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
纯度 >97%(SDS-PAGE and HPLC)
蛋白标签 No tag
蛋白长度 Full length protein
无载体 Yes
无动物源 Yes
Accession # Q07654
来源 重组表达
预测分子量 13.2 kDa
SDS-PAGE 13.2 kDa

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 使用手动除霜冰柜,避免反复冻融循环。自收到之日起 12 个月,-20 至 -70 °C。复溶后 1 个月,无菌条件下 2 至 8 °C。3 个月,复溶后在无菌条件下 -20 至 -70 °C。
分子类型 蛋白质

图片

Recombinant Human Trefoil Factor 3 Protein (rp152656)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/mL, corresponding to a specific activity of > 100 IU/mg.

Recombinant Human Trefoil Factor 3 Protein (rp152656)-SDS-PAGE
Recombinant Human Trefoil Factor 3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a single band at 14.5 kDa under reducing conditions and 29 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F0600356 分析证书 rp152656
ZJ23F0600357 分析证书 rp152656
ZJ23F0600358 分析证书 rp152656

引用文献

1. Hauser, F F and 6 more authors..  (1993)  hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus..  Proceedings of the National Academy of Sciences of the United States of America,  (1): [PMID:8346203]
2. Podolsky, D K DK and 7 more authors..  (1993)  Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion..  The Journal of biological chemistry,  (25): [PMID:8454642]
3. Seib, T T and 8 more authors..  (1997)  The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3..  Genomics,  (15): [PMID:9070946]
4. Hattori, M M and 64 more authors..  (2000)  The DNA sequence of human chromosome 21..  Nature,  (18): [PMID:10830953]
5. Berry, A A and 16 more authors..  (2000)  Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region..  Genomics,  (15): [PMID:10950923]
6. Oertel, M M and 5 more authors..  (2001)  Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor..  American journal of respiratory cell and molecular biology,  [PMID:11694446]
7. Casadei, Raffaella R and 10 more authors..  (2003)  mRNA 5' region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs..  Gene,  (4): [PMID:14637006]
8. Muskett, Frederick W FW, May, Felicity E B FE, Westley, Bruce R BR and Feeney, James J..  (2003)  Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins..  Biochemistry,  (30): [PMID:14690424]
9. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,  [PMID:15340161]

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