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Recombinant Human TFF3 Protein

  • 表达系统: HEK293
  • Accession #: Q07654
  • 蛋白标签: C-His
  • 生物活性: Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF3 can effectively induce ERK1/2 phosphorylation.
  • 内毒素水平: <0.1 EU/μg
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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp152655-10μg
10μg 现货 Stock Image
rp152655-50μg
50μg 现货 Stock Image
rp152655-100μg
100μg 现货 Stock Image
rp152655-1mg
1mg 期货 Stock Image

基本描述

产品名称 Recombinant Human TFF3 Protein
别名 重组人TFF3蛋白 | 重组人三叶因子3蛋白 | 重组人TFF3蛋白 | 重组人三叶因子3蛋白
英文别名 hITF | hP1.B | Intestinal trefoil factor | Polypeptide P1.B | HITF | human intestinal trefoil factor | Intestinal trefoil factor | ITF | TFF3 | TFI | trefoil factor 3 (intestinal) | P1B | human intestinal trefoil factor1 | HITF | Intestinal trefoil factor
规格或纯度 无动物源, Carrier Free, 生物活性, ActiBioPure™, High performance, ≥90%(SDS-PAGE)
产品介绍


Function
Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).

生物活性 Measured by its ability to induce ERK1/ERK2 phosphorylation in Jurkat human acute T cell leukemia cells. 5-15 μg/mL of Recombinant Human TFF3 can effectively induce ERK1/2 phosphorylation.
内毒素水平 <0.1 EU/μg
表达系统 HEK293
种属 人(Human)
氨基酸 22-80 aa
序列 EEYVGLSANQCAVPAKDRVDCGYPHVTPKECNNRGCCFDSRIPGVPWCFKPLQEAECTFHHHHHH
蛋白标签 C-His
无载体 Yes
无动物源 Yes
Accession # Q07654
来源 重组表达
预测分子量 7.4 kDa
SDS-PAGE 11 kDa, under reducing conditions; 12 & 18 kDa, under non-reducing conditions.

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4.
复溶 Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型 蛋白质

图片

Recombinant Human TFF3 Protein (rp152655) - SDS-PAGE
2 μg/lane of Recombinant Human TFF3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 11 kDa under reducing condition and 12 & 18 kDa under non-reducing condition.

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到2个结果

批号(Lot Number) 证书类型 货号
ZJ25F0420471 分析证书 rp152655
ZJ25F0420470 分析证书 rp152655

引用文献

1. Hauser, F F and 6 more authors..  (1993)  hP1.B, a human P-domain peptide homologous with rat intestinal trefoil factor, is expressed also in the ulcer-associated cell lineage and the uterus..  Proceedings of the National Academy of Sciences of the United States of America,  (1): [PMID:8346203]
2. Podolsky, D K DK and 7 more authors..  (1993)  Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion..  The Journal of biological chemistry,  (25): [PMID:8454642]
3. Seib, T T and 8 more authors..  (1997)  The three human trefoil genes TFF1, TFF2, and TFF3 are located within a region of 55 kb on chromosome 21q22.3..  Genomics,  (15): [PMID:9070946]
4. Hattori, M M and 64 more authors..  (2000)  The DNA sequence of human chromosome 21..  Nature,  (18): [PMID:10830953]
5. Berry, A A and 16 more authors..  (2000)  Refined localization of autosomal recessive nonsyndromic deafness DFNB10 locus using 34 novel microsatellite markers, genomic structure, and exclusion of six known genes in the region..  Genomics,  (15): [PMID:10950923]
6. Oertel, M M and 5 more authors..  (2001)  Trefoil factor family-peptides promote migration of human bronchial epithelial cells: synergistic effect with epidermal growth factor..  American journal of respiratory cell and molecular biology,  [PMID:11694446]
7. Casadei, Raffaella R and 10 more authors..  (2003)  mRNA 5' region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs..  Gene,  (4): [PMID:14637006]
8. Muskett, Frederick W FW, May, Felicity E B FE, Westley, Bruce R BR and Feeney, James J..  (2003)  Solution structure of the disulfide-linked dimer of human intestinal trefoil factor (TFF3): the intermolecular orientation and interactions are markedly different from those of other dimeric trefoil proteins..  Biochemistry,  (30): [PMID:14690424]
9. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,  [PMID:15340161]

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