Recombinant Human IL-21 Protein

COA

级别和纯度:

≥97%(SDS-PAGE&HPLC)

表达系统: E. coli
Accession #: Q9HBE4
蛋白标签: No tag
生物活性: 1. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 2.0 ng/mL. 2. Recombinant Human IL-21 stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED50 for this effect is typically less than 40 ng/mL.
内毒素水平: <0.01 EU/μg

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货号 (SKU)	包装规格	是否现货
rp147498-10μg	10μg	现货
rp147498-20μg	20μg	现货
rp147498-100μg	100μg	现货
rp147498-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL21

Accession#:Q9HBE4 (6) Gene ID:59067 (6) IL21 (6)

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基本描述

产品名称	Recombinant Human IL-21 Protein
别名	重组人IL-21蛋白 \| 重组人 IL-21 蛋白
英文别名	CVID11 \| IL21 \| IL-21 \| IL-21Za11interleukin-21 \| interleukin 21 \| interleukin-21 isoform \| Za11 \| IL-21 \| Za11
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
产品介绍	Purity >97%（SDS-PAGE,HPLC） Function Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
生物活性	1. Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED50 for this effect is ≤ 2.0 ng/mL. 2. Recombinant Human IL-21 stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED50 for this effect is typically less than 40 ng/mL.
内毒素水平	<0.01 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	30-162 aa
序列	QGQDRHMIRMRQLIDIVDQLKNYVNDLVPEFLPAPEDVETNCEWSAFSCFQKAQLKSANTGNNERIINVSIKKLKRKPPSTNAGRRQKHRLTCPSCDSYEKKPPKEFLERFKSLLQKMIHQHLSSRTHGSEDS
纯度	＞97% (SDS-PAGE, HPLC)
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	Q9HBE4
来源	重组表达
预测分子量	15.4 kDa
SDS-PAGE	13.9 kDa, under reducing conditions; 13.9 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2μm filtered solution 20mM PBS, pH 7.4
复溶	This vial must be briefly centrifuged prior to opening to bring the - 2 - contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	如需长期储存，产品应储存在 ≤ -20°C。复溶后请避免反复冻融循环。自收到之日起 12 个月，-20 至 -70℃（按提供时的温度）。 1 个月，复溶后在无菌条件下 2 至 8℃ 保存
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IL-21 Protein (rp147498)

Recombinant Human IL-21 Protein (rp147498) - Protein Bioactivity
Recombinant Human IL-21 stimulates cell proliferation of the CTLL-2 mouse cytotoxic T cell line. The ED₅₀ for this effect is typically less than 40 ng/mL.

Protein Bioactivity: Recombinant Human IL-21 Protein (rp147498)

Recombinant Human IL-21 Protein (rp147498) - Protein Bioactivity
Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is less than 2.0 ng/mL.

SDS-PAGE: Recombinant Human IL-21 Protein (rp147498)

Recombinant Human IL-21 Protein (rp147498) - SDS-PAGE
2 μg/lane of Recombinant Human IL-21 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.9 kDa.

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找到5个结果

批号(Lot Number)	证书类型	货号
ZJ23F0901078	分析证书	rp147498
ZJ23F0901079	分析证书	rp147498
ZJ25F0420923	分析证书	rp147498
ZJ25F0420924	分析证书	rp147498
ZJ25F0420925	分析证书	rp147498

技术文档和文章

引用文献

1. Parrish-Novak, J J and 37 more authors.. (2000) Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function.. Nature, (2): [PMID:11081504]
2. Sivakumar, Pallavur V PV, Foster, Donald C DC and Clegg, Christopher H CH.. (2004) Interleukin-21 is a T-helper cytokine that regulates humoral immunity and cell-mediated anti-tumour responses.. Immunology, [PMID:15147560]
3. Strengell, Mari M, Julkunen, Ilkka I and Matikainen, Sampsa S.. (2004) IFN-alpha regulates IL-21 and IL-21R expression in human NK and T cells.. Journal of leukocyte biology, [PMID:15178704]
4. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621]
5. Bondensgaard, Kent K and 8 more authors.. (2007) The existence of multiple conformers of interleukin-21 directs engineering of a superpotent analogue.. The Journal of biological chemistry, (10): [PMID:17565991]
6. Rahman, Mizanur M and 6 more authors.. (2007) Cloning and characterization of an isoform of interleukin-21.. FEBS letters, (21): [PMID:17673207]
7. Hamming, Ole J OJ and 8 more authors.. (2012) Crystal structure of interleukin-21 receptor (IL-21R) bound to IL-21 reveals that sugar chain interacting with WSXWS motif is integral part of IL-21R.. The Journal of biological chemistry, (16): [PMID:22235133]
8. Salzer, Elisabeth E and 17 more authors.. (2014) Early-onset inflammatory bowel disease and common variable immunodeficiency-like disease caused by IL-21 deficiency.. The Journal of allergy and clinical immunology, [PMID:24746753]

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Recombinant Human IL-21 Protein

库存信息

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库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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引用文献

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