货号 (SKU)

包装规格

是否现货

价格

数量

rp144122-25μg

25μg

现货

rp144122-100μg

100μg

现货

rp144122-1mg

1mg

期货

基本描述

产品名称	Recombinant Human CD80 Protein
别名	重组人 CD80 蛋白
英文别名	Activation B7-1 antigen \| B7 \| BB1 \| CTLA-4 counter-receptor B7.1
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity >95% SDS-PAGE. Molecular weight informat The protein migrates as 65-90 kDa under reducing condition due to glycosylation. Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
生物活性	Measured by its binding activity of recombinant human CD80 and recombinant human CD86. The EC50 for this effect is less than 0.412μg/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	35-242 aa
序列	VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIW PEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEV TLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEEL NAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFN WNTTKQEHFPDN
纯度	＞95% (SDS-PAGE)
蛋白标签	N-His
蛋白长度	Protein fragment
无载体	Yes
无动物源	Yes
Accession #	P33681
来源	重组表达
预测分子量	25.5 kDa

储存与运输

应用	Cell culture; Activity Assays
物理形态	冻干(Lyophilized)
储存缓冲液	PBS, pH7.4, containing 5% trehalose.
复溶	Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not Vortex.
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	避免反复冻融循环。在 2-8 ℃ 下保存一个月。分装后在 -80 ℃ 下保存 12 个月。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human CD80 Protein (rp144122)

Recombinant Human CD80 Protein (rp144122) - Protein Bioactivity
Measured by its binding activity of recombinant human CD80 and recombinant human CD86. The EC₅₀ for this effect is less than 0.412 μg/mL.

SDS-PAGE: Recombinant Human CD80 Protein (rp144122)

Recombinant Human CD80 Protein (rp144122) - SDS-PAGE
2.5 μg/lane of Recombinant Human CD80 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 50.2 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ23F0700615	分析证书	rp144122
ZJ23F0700614	分析证书	rp144122

技术文档和文章

阿拉丁®靶点蛋白

Categories: 技术文章

探索主题:

药物开发信号通路细胞因子靶点蛋白药物靶点受体免疫检查点细胞表面标记物

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引用文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334]
2. Vicente Rabaneda EF, Herrero-Beaumont G, Castañeda S. (2013) Update on the use of abatacept for the treatment of rheumatoid arthritis.. Expert Rev Clin Immunol, 9 (7): (599-621). [PMID:23899231]
3. Linsley PS, Brady W, Urnes M, Grosmaire LS, Damle NK, Ledbetter JA. (1991) CTLA-4 is a second receptor for the B cell activation antigen B7.. J Exp Med, 174 (3): (561-9). [PMID:1714933]
4. Selvakumar, A A and 5 more authors.. (1992) Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7.. Immunogenetics, [PMID:1377173]
5. Freeman, G J GJ and 8 more authors.. (1991) Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7.. The Journal of experimental medicine, (1): [PMID:1714935]
6. Freeman, G J GJ and 5 more authors.. (1989) B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2794510]
7. Lanier, L L LL and 7 more authors.. (1995) CD80 (B7) and CD86 (B70) provide similar costimulatory signals for T cell proliferation, cytokine production, and generation of CTL.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:7527824]
8. Vandenborre, K K and 5 more authors.. (1999) Interaction of CTLA-4 (CD152) with CD80 or CD86 inhibits human T-cell activation.. Immunology, [PMID:10583602]
9. Ikemizu, S S and 7 more authors.. (2000) Structure and dimerization of a soluble form of B7-1.. Immunity, [PMID:10661405]
10. Stamper, C C CC and 9 more authors.. (2001) Crystal structure of the B7-1/CTLA-4 complex that inhibits human immune responses.. Nature, (29): [PMID:11279502]
11. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ.. (2002) TTD: Therapeutic Target Database.. Nucleic acids research, (1): [PMID:11752352]
12. Gottlieb, Alice B AB and 8 more authors.. (2004) Evaluation of safety and clinical activity of multiple doses of the anti-CD80 monoclonal antibody, galiximab, in patients with moderate to severe plaque psoriasis.. Clinical immunology (Orlando, Fla.), [PMID:15093549]
13. Czuczman, Myron S MS and 13 more authors.. (2005) Phase I/II study of galiximab, an anti-CD80 antibody, for relapsed or refractory follicular lymphoma.. Journal of clinical oncology : official journal of the American Society of Clinical Oncology, (1): [PMID:15994148]
14. and Kremer, Joel M JM.. (2005) Selective costimulation modulators: a novel approach for the treatment of rheumatoid arthritis.. Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, [PMID:16357751]
15. Hervey, Pauline S PS and Keam, Susan J SJ.. (2006) Abatacept.. BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy, [PMID:16573350]
16. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997]
17. Weyand, Cornelia M CM and Goronzy, Jörg J JJ.. (2006) T-cell-targeted therapies in rheumatoid arthritis.. Nature clinical practice. Rheumatology, [PMID:16932686]
18. and Scheinfeld, Noah N.. (2006) Abatacept: A review of a new biologic agent for refractory rheumatoid arthritis for dermatologists.. The Journal of dermatological treatment, [PMID:16971318]
19. Tedesco Silva, Helio H, Pinheiro Machado, Paula P, Rosso Felipe, Claudia C and Medina Pestana, Jose Osmar JO.. (2006) Immunotherapy for De Novo renal transplantation: what's in the pipeline?. Drugs, [PMID:16978033]
20. Vincenti, Flavio F and Luggen, Michael M.. (2007) T cell costimulation: a rational target in the therapeutic armamentarium for autoimmune diseases and transplantation.. Annual review of medicine, [PMID:17020493]
21. Nogid, Anna A and Pham, David Q DQ.. (2006) Role of abatacept in the management of rheumatoid arthritis.. Clinical therapeutics, [PMID:17212998]
22. Leonard, J P JP and 14 more authors.. (2007) A phase I/II study of galiximab (an anti-CD80 monoclonal antibody) in combination with rituximab for relapsed or refractory, follicular lymphoma.. Annals of oncology : official journal of the European Society for Medical Oncology, [PMID:17470451]
23. Kakoulidou, M M, Giscombe, R R, Zhao, X X, Lefvert, A K AK and Wang, X X.. (2007) Human Soluble CD80 is generated by alternative splicing, and recombinant soluble CD80 binds to CD28 and CD152 influencing T-cell activation.. Scandinavian journal of immunology, [PMID:17953528]
24. Reynolds, Jennifer J, Shojania, Kam K and Marra, Carlo A CA.. (2007) Abatacept: a novel treatment for moderate-to-severe rheumatoid arthritis.. Pharmacotherapy, [PMID:18041889]
25. Maxwell, Lara J LJ and Singh, Jasvinder A JA.. (2010) Abatacept for rheumatoid arthritis: a Cochrane systematic review.. The Journal of rheumatology, [PMID:20080922]
26. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
27. Kleinpeter, Patricia and 20 more authors.. (2019) By Binding CD80 and CD86, the Vaccinia Virus M2 Protein Blocks Their Interactions with both CD28 and CTLA4 and Potentiates CD80 Binding to PD-L1.. Journal of virology, (1): [PMID:30918073]

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体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Human CD80 Protein

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器