Recombinant Human B7-1/CD80 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P33681
蛋白标签: C-His
生物活性: Immobilized Recombinant Human B7-1/CD80 Protein (rp144120) at 2.0 μg/mL can bind Recombinant human CTLA-4 with a linear range of 7.0 - 62.0 ng/mL. The EC50 for this effect is 23.47 ng/mL.
内毒素水平: <0.1 EU/μg

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp144120-10μg	10μg	现货
rp144120-50μg	50μg	现货
rp144120-100μg	100μg	现货
rp144120-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CD80

Accession#:P33681 (5) B7-1 (2) CD80 (9) Gene ID:941 (6)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	Recombinant Human B7-1/CD80 Protein
别名	B-淋巴细胞激活抗原B7-1(LAB7-1)活性蛋白 \| 重组人 B7-1/CD80 蛋白
英文别名	Activation B7-1 antigen \| B7 \| BB1 \| CTLA-4 counter-receptor B7.1 \| Activation B7-1 antigen \| B lymphocyte activation antigen B7 \| B7 \| B7-1 \| B7-1 antigen \| B7.1 \| BB1 \| CD28 antigen ligand 1 \| CD28LG \| CD28LG1 \| CD80 \| CD80 antigen \| CD80 antigen (CD28
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% (SDS-PAGE) Endotoxin level <0.1 EU/μg Function Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor.
生物活性	Immobilized Recombinant Human B7-1/CD80 Protein (rp144120) at 2.0 μg/mL can bind Recombinant human CTLA-4 with a linear range of 7.0 - 62.0 ng/mL. The EC50 for this effect is 23.47 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	35-242 aa
序列	VIHVTKEVKEVATLSCGHNVSVEELAQTRIYWQKEKKMVLTMMSGDMNIWPEYKNRTIFDITNNLSIVILALRPSDEGTYECVVLKYEKDAFKREHLAEVTLSVKADFPTPSISDFEIPTSNIRRIICSTSGGFPEPHLSWLENGEELNAINTTVSQDPETELYAVSSKLDFNMTTNHSFMCLIKYGHLRVNQTFNWNTTKQEHFPDNHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P33681
来源	重组表达
预测分子量	25 kDa
SDS-PAGE	40 - 55 kDa, under reducing conditions; 40 - 55 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human B7-1/CD80 Protein (rp144120)

Recombinant Human B7-1/CD80 Protein (rp144120) - Protein Bioactivity
Immobilized Recombinant Human B7-1/CD80 Protein (rp144120) at 2.0 μg/mL can bind Recombinant human CTLA-4 with a linear range of 7.0 - 62.0 ng/mL. The EC50 for this effect is 23.47 ng/mL.

SDS-PAGE: Recombinant Human B7-1/CD80 Protein (rp144120)

Recombinant Human B7-1/CD80 Protein (rp144120) - SDS-PAGE
3 μg/lane of Recombinant Human B7-1/CD80 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40 - 55 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到6个结果

批号(Lot Number)	证书类型	货号
ZJ24F1215480	分析证书	rp144120
ZJ24F1215478	分析证书	rp144120
ZJ24F1215479	分析证书	rp144120
ZJ24F0809593	分析证书	rp144120
ZJ24F0809591	分析证书	rp144120
ZJ24F0809592	分析证书	rp144120

技术文档和文章

引用文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334]
2. Vicente Rabaneda EF, Herrero-Beaumont G, Castañeda S. (2013) Update on the use of abatacept for the treatment of rheumatoid arthritis.. Expert Rev Clin Immunol, 9 (7): (599-621). [PMID:23899231]
3. Linsley PS, Brady W, Urnes M, Grosmaire LS, Damle NK, Ledbetter JA. (1991) CTLA-4 is a second receptor for the B cell activation antigen B7.. J Exp Med, 174 (3): (561-9). [PMID:1714933]
4. Selvakumar, A A and 5 more authors.. (1992) Genomic organization and chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7.. Immunogenetics, [PMID:1377173]
5. Freeman, G J GJ and 8 more authors.. (1991) Structure, expression, and T cell costimulatory activity of the murine homologue of the human B lymphocyte activation antigen B7.. The Journal of experimental medicine, (1): [PMID:1714935]
6. Freeman, G J GJ and 5 more authors.. (1989) B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:2794510]
7. Lanier, L L LL and 7 more authors.. (1995) CD80 (B7) and CD86 (B70) provide similar costimulatory signals for T cell proliferation, cytokine production, and generation of CTL.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:7527824]
8. Vandenborre, K K and 5 more authors.. (1999) Interaction of CTLA-4 (CD152) with CD80 or CD86 inhibits human T-cell activation.. Immunology, [PMID:10583602]
9. Ikemizu, S S and 7 more authors.. (2000) Structure and dimerization of a soluble form of B7-1.. Immunity, [PMID:10661405]
10. Gottlieb, Alice B AB and 8 more authors.. (2004) Evaluation of safety and clinical activity of multiple doses of the anti-CD80 monoclonal antibody, galiximab, in patients with moderate to severe plaque psoriasis.. Clinical immunology (Orlando, Fla.), [PMID:15093549]
11. Czuczman, Myron S MS and 13 more authors.. (2005) Phase I/II study of galiximab, an anti-CD80 antibody, for relapsed or refractory follicular lymphoma.. Journal of clinical oncology : official journal of the American Society of Clinical Oncology, (1): [PMID:15994148]
12. and Kremer, Joel M JM.. (2005) Selective costimulation modulators: a novel approach for the treatment of rheumatoid arthritis.. Journal of clinical rheumatology : practical reports on rheumatic & musculoskeletal diseases, [PMID:16357751]
13. Hervey, Pauline S PS and Keam, Susan J SJ.. (2006) Abatacept.. BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy, [PMID:16573350]
14. Muzny, Donna M DM and 113 more authors.. (2006) The DNA sequence, annotation and analysis of human chromosome 3.. Nature, (27): [PMID:16641997]
15. Weyand, Cornelia M CM and Goronzy, Jörg J JJ.. (2006) T-cell-targeted therapies in rheumatoid arthritis.. Nature clinical practice. Rheumatology, [PMID:16932686]
16. and Scheinfeld, Noah N.. (2006) Abatacept: A review of a new biologic agent for refractory rheumatoid arthritis for dermatologists.. The Journal of dermatological treatment, [PMID:16971318]
17. Tedesco Silva, Helio H, Pinheiro Machado, Paula P, Rosso Felipe, Claudia C and Medina Pestana, Jose Osmar JO.. (2006) Immunotherapy for De Novo renal transplantation: what's in the pipeline?. Drugs, [PMID:16978033]
18. Vincenti, Flavio F and Luggen, Michael M.. (2007) T cell costimulation: a rational target in the therapeutic armamentarium for autoimmune diseases and transplantation.. Annual review of medicine, [PMID:17020493]
19. Nogid, Anna A and Pham, David Q DQ.. (2006) Role of abatacept in the management of rheumatoid arthritis.. Clinical therapeutics, [PMID:17212998]
20. Leonard, J P JP and 14 more authors.. (2007) A phase I/II study of galiximab (an anti-CD80 monoclonal antibody) in combination with rituximab for relapsed or refractory, follicular lymphoma.. Annals of oncology : official journal of the European Society for Medical Oncology, [PMID:17470451]
21. Kakoulidou, M M, Giscombe, R R, Zhao, X X, Lefvert, A K AK and Wang, X X.. (2007) Human Soluble CD80 is generated by alternative splicing, and recombinant soluble CD80 binds to CD28 and CD152 influencing T-cell activation.. Scandinavian journal of immunology, [PMID:17953528]
22. Reynolds, Jennifer J, Shojania, Kam K and Marra, Carlo A CA.. (2007) Abatacept: a novel treatment for moderate-to-severe rheumatoid arthritis.. Pharmacotherapy, [PMID:18041889]
23. Maxwell, Lara J LJ and Singh, Jasvinder A JA.. (2010) Abatacept for rheumatoid arthritis: a Cochrane systematic review.. The Journal of rheumatology, [PMID:20080922]
24. Kleinpeter, Patricia and 20 more authors.. (2019) By Binding CD80 and CD86, the Vaccinia Virus M2 Protein Blocks Their Interactions with both CD28 and CTLA4 and Potentiates CD80 Binding to PD-L1.. Journal of virology, (1): [PMID:30918073]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Human B7-1/CD80 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器