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Casein-FITC conjugate

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
C275894-5mg
5mg 现货 Stock Image
C275894-25mg
25mg 期货 Stock Image
C275894-100mg
100mg 期货 Stock Image

基本描述

产品名称 Casein-FITC conjugate
别名 酪蛋白异硫氰酸荧光素
英文别名 FITC-Casein | fluorescein isothiocyanate (FITC)-labeled casein
规格或纯度 Ex:498nm, Em:517nm
产品介绍


Casein is considered to be a generic substrate for a broad spectrum of proteases. As native casein this fluoresceinated casein is hydrolyzed by many proteases, and widely used for fluorimetric measurement of protease activity. In the intact substrate, casein is heavily labeled with 5-FITC, resulting in significant fluorescence quenching. Protease-catalyzed hydrolysis relieves its quenching effect, yielding brightly green fluorescent dye-labeled short peptides. The increase in fluorescence intensity is directly proportional to protease activity. We do not recommend that this conjugate be used for fluorescence polarization assay. For fluorescence polarization we can custom-make the lightly labeled fluorescein casein conjugate.

偶联 FITC
Excitation(nm) Blue Laser(488nm)
Emission(nm) 517nm
Ex/Em(nm) Ex:498nm;Em:517nm

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from 25mM Tris, pH 7.2
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the material to approximately 5 mg/ml by addition of 1mL distilled water. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C, avoid repeated freeze-thaw cycles.
储存温度 2-8°C储存,避光
运输条件 冰袋运输
稳定性与储存 Product should be stored at 2-8℃ in the dark. After reconstitution, store in aliquots and freeze material at -20℃, avoid freeze/thaw cycle.
分子类型 蛋白质

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批号(Lot Number) 证书类型 货号
ZJ24F1214770 分析证书 C275894

引用文献

1. Narayanan A et al..  (2011)  Restrained expression, a method to overproduce toxic membrane proteins by exploiting operator-repressor interactions..  Protein Sci,  20  (51-61).  [PMID:21031485]

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