Recombinant EN-TEV Protease Protein

COA

级别和纯度:

≥98%(SDS-PAGE)
见COA

表达系统: E. coli
Accession #: P04517
蛋白标签: N-His
生物活性: Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT.
内毒素水平: <1.0 EU/μg

有货

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
rp156465-1KU	1KU	现货
rp156465-10KU	10KU	现货

查看相关系列

Accession#:P04517 (2) EN-TEV Protease (2) Gene ID:1502321 (2) TEVgp1 (2)

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基本描述

产品名称	Recombinant EN-TEV Protease Protein
别名	重组EN-TEV Protease蛋白 \| 重组 EN-TEV 蛋白酶蛋白
英文别名	NIa \| P1 Protease \| TEV Protease \| Tobacco Etch Virus Protease
规格或纯度	无载体, 生物活性, ActiBioPure™, 无叠氮钠, ≥98%(SDS-PAGE), 见COA
产品介绍	TEV Protease is the 241 amino acid (aa), 27 kDa catalytic domain of the nuclear inclusion a (NIa) protein encoded by the potyvirus, tobacco etch virus (TEV). It may be used in biotechnology to cleave affinity tags from recombinant proteins, either co-translationally orin vitrofollowing purification. Its high specificity and activity at a wide range of pH and ionic strength make TEV Protease more versatile than many other proteases used for the same purpose. Unlike factor Xa, enteropeptidase or thrombin, TEV Protease has not been found to cleave at unintended sites, even when present at a high concentration. TEV Protease is a 3C-type protease that cleaves substrates with a consensus sequence of ENLYFQG. Cleavage occurs between Q and G. Since the final aa remains on the cleaved protein where it could potentially affect structure or function, substitution of a variety of aa have been tested. In order of efficiency, S, A, M, Y, D, N, E, K or L may be effectively used in place of G. Several of the remaining aa may also vary, giving a final consensus sequence of ExxYF(M)Q(E)/G(S, A or others) where aa in parenthesis are alternatives and x is any aa. The autocatalytic site of NIa at S2256 has been mutated to an N for improved stability of the protease. Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the tags from fusion proteins. The optimal temperature for cleavage is 30°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
生物活性	Measured by its ability to cleave a fusion protein containing the recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser , with the cleavage point after Gln. One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C.It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature.Complete digestion of 50µg of fusion KGF-MBP tag protein using 1µg TEV enzyme (in 1/50 ratio) in either 1 hours at 30 °C or overnight at 4 °C. The "standard" reaction buffer for TEV protease is 50 mM Tris-HCl (pH 8.0), 0.5 mM EDTA and 1mM DTT.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	烟草蚀纹病毒(Tobacco etch virus(TEV))
氨基酸	2038-2279 aa
序列	GHHHHHHHGESLFKGPRDYNPISSSICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLVVQSLHGVFKVKDTTTLQQHLVDERDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSSTFPSGDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFANTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNRRRRR
纯度	＞98% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
Accession #	P04517
来源	重组表达
预测分子量	28.6 kDa
SDS-PAGE	28.6 kDa

储存与运输

物理形态	液体
储存缓冲液	25mM HEPES buffer, 400mM NaCl, PH7.5
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant EN-TEV Protease protein (rp156465)

Recombinant EN-TEV Protease protein (rp156465) - SDS-PAGE
3 μg/lane of Recombinant EN-TEV Protease protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.6 kDa.

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ23F0800865	分析证书	rp156465
ZJ23F0800864	分析证书	rp156465

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