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ROX参比染料

    级别和纯度:
  • 25µmol/L
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货号 (SKU) 包装规格 是否现货 价格 数量
R272949-1ml
1ml 现货 Stock Image
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染料 (55)

基本描述

规格或纯度 25µmol/L
英文名称 ROX Reference Dye
储存温度 避光,-20°C储存,干燥
运输条件 超低温冰袋运输
产品介绍

Ex(nm):568      Em(nm):591

制品说明
ROX Reference Dye一般用于ABI、Stratagene等公司的Real Time PCR 扩增仪上,用于调整PCR加样误差所引起的PCR管与管之间的差异。不同仪器所需ROX Reference Dye浓度不同。我公司提供ROX Reference Dye 储存液,浓度为25µmol/L,使用终浓度根据不同仪器为50nmol/L 或500nmol/L,用户可以根据仪器的推荐浓度添加。

不同仪器ROX推荐使用终浓度:

Instrument

ROX per 50µL PCR Reaction

Final Concentration

ABI PRISM 7000/7300/7700/7900HT and 7900HT Fast, ABI Step One, ABI Step One Plus

1.0 µL(0.6~1.0µL)

500nmol/L(300~500nmol/L)

ABI 7500, 7500   Fast, Stratagene Mx3000P, Mx3005P, Mx4000, MJ Research Chromo4, Opticon (II), Corbett   Rotor Gene 3000

0.1 µL*(0.06~0.1µL)

50nmol/L(30~50nmol/L)

 从配制PCR反应的水溶液中减去ROX染料的体积

*为使每个反应精确加入0.1µL,推荐使用前将ROX用水稀释10倍,并使用1µL稀释后的溶液。

Ex(nm):568      Em(nm):591

Product description

ROX Reference Dye is generally used on Real Time PCR amplification machines of companies such as ABI and Stratagene to adjust the difference between PCR tubes and tubes caused by PCR sample loading errors. Different instruments require different concentrations of ROX Reference Dye. Our company provides ROX Reference Dye storage solution with a concentration of 25μmol/L, and the final concentration of use is 50nmol/L or 500nmol/L depending on the instrument. The user can add it according to the recommended concentration of the instrument.

The recommended final concentration of ROX for different instruments:

Instrument

ROX per 50µL PCR Reaction

Final Concentration

ABI PRISM 7000/7300/7700/7900HT and 7900HT Fast, ABI Step One, ABI Step One Plus

1.0 µL(0.6~1.0µL)

500nmol/L(300~500nmol/L)

ABI 7500, 7500   Fast, Stratagene Mx3000P, Mx3005P, Mx4000, MJ Research Chromo4, Opticon (II), Corbett   Rotor Gene 3000

0.1 µL*(0.06~0.1µL)

50nmol/L(30~50nmol/L)

Subtract the volume of ROX dye from the aqueous solution used to prepare the PCR reaction

*In order to accurately add 0.1μL to each reaction, it is recommended to dilute ROX 10 times with water before use, and use 1μL of the diluted solution.

化学和物理性质

敏感性 对光和湿度敏感

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批号(Lot Number) 证书类型 货号
I2314345 分析证书 R272949
F2417213 分析证书 R272949
E2428394 分析证书 R272949
G2303293 分析证书 R272949

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