| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| cl155900-200μg |
200μg |
现货  |
|
| 产品名称 | NIH/3T3 Whole Cell Lysate |
|---|---|
| 别名 | NIH/3T3 全细胞裂解物 |
| 规格或纯度 | Carrier Free, Azide Free, 200μg, Mycoplasma free |
| 产品介绍 |
Product Description： The NIH/3T3 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. NIH/3T3 cells are established from a NIH Swiss mouse embryo. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition. This cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. The established NIH/3T3 line was subjected to more than 5 serial cycles of subcloning in order to develop a subclone with morphologic characteristics best suited for transformation assays. It is therefore used for DNA transfection studies. |
| 应用 | WB |
|---|---|
| 物理形态 | 冻干(Lyophilized) |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4). |
| 浓度 | 200μg, Mycoplasma free |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
| 分子类型 | 细胞或组织提取物 |
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
NIH/3T3 Whole Cell Lysate (cl155900) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F0800808 |
分析证书 | cl155900 |
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