| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| cl155898-200μg |
200μg |
现货  |
|
| 产品名称 | K-562 Whole Cell Lysate |
|---|---|
| 别名 | K-562 全细胞裂解物 |
| 英文别名 | K562 Whole Cell Lysate |
| 规格或纯度 | Carrier Free, Azide Free, 200μg, Mycoplasma free |
| 产品介绍 |
Product Description： The K-562 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays. |
| 应用 | WB |
|---|---|
| 物理形态 | 冻干(Lyophilized) |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4) |
| 浓度 | 200μg, Mycoplasma free |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
| 分子类型 | 细胞或组织提取物 |
K562 Whole Cell Lysate (cl155898) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: K562 Whole Cell Lysate (cl155898)
Lane 2: HEK293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
K-562 Whole Cell Lysate (cl155898) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
K-562 Whole Cell Lysate (cl155898) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: K-562 Whole Cell Lysate (cl155898)
Lane 2: HEK-293 Whole Cell Lysate (cl155895)
Lane 3: A431 Whole Cell Lysate (cl155901)
Lane 4: NIH/3T3 Whole Cell Lysate (cl155900)
Lane 5: HT-29 Whole Cell Lysate (cl155904)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F0800805 |
分析证书 | cl155898 |
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