| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| cl155896-200μg |
200μg |
现货  |
|
| 产品名称 | Hep G2 Whole Cell Lysate |
|---|---|
| 别名 | Hep G2全细胞裂解物 | HepG2全细胞裂解物 | Hep G2 全细胞裂解物 |
| 英文别名 | HepG2 Whole Cell Lysate |
| 规格或纯度 | Carrier Free, Azide Free, 200μg, Mycoplasma free |
| 产品介绍 |
Product Description： The Hep G2 whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process. Hep G2 cells are ability to differentiate into polarized epithelium, these cells are routinely utilized to investigate intracellular protein trafficking, particularly in relation to human liver diseases. HepG2 is the most commonly used cell line in studies pertaining to the regulation of hepatic protein synthesis, particularly the synthesis of acute phase proteins after the onset of a systemic inflammatory response. In addition, these cells are important for studies related to liver metabolism, liver cancers, liver regeneration, liver cytotoxicity, apoptosis and as a model for hepatitis B virus (HBV) viral etiology. |
| 应用 | WB |
|---|---|
| 物理形态 | 冻干(Lyophilized) |
| 储存缓冲液 | Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail (EDTA-Free Protease Inhibitor Cocktail) and phosphatase inhibitor (Na3VO4). |
| 浓度 | 200μg, Mycoplasma free |
| 复溶 | Recommended reconstitute at 1-2 mg/mL in ddH2O. Use at an assay dependent concentration. Ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. |
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Shipped on Dry Ice. Lyophilizate is recommended stored at -80℃. After reconstitution, recommended aliquot and avoid repeated freeze-thaw, store at -20℃ for short-term storage or -80℃ for long-term storage. |
| 分子类型 | 细胞或组织提取物 |
Hep G2 Whole Cell Lysate (cl155896) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Hep G2 Whole Cell Lysate (cl155896) - Western Blot
All lanes: PCNA Mouse mAb (Ab155826) at 1/2000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Hep G2 Whole Cell Lysate (cl155896) - Western Blot
All lanes: Heat Shock Protein 60 antibody (HSP60) at 1/1000 dilution
Lane 1: A549 Whole Cell Lysate (cl155906)
Lane 2: MCF7 Whole Cell Lysate (cl155899)
Lane 3: HeLa Whole Cell Lysate (cl155894)
Lane 4: Hep G2 Whole Cell Lysate (cl155896)
Lane 5: Raji Whole Cell Lysate (cl155907)
Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 60 kDa
Observed band size: 58 kDa
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ23F0500179 |
分析证书 | cl155896 |
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