胶原酶 NB 5 来源于溶组织梭菌

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COA

级别和纯度:

EnzymoPure™

无菌

≥0.10 U/mg

Accession #: P43153,Q46085,Q9X721
生物活性: ≥0.10 U/mg

有货

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货号 (SKU)	包装规格	是否现货
C279009-10mg	10mg	现货
C279009-100mg	100mg	现货
C279009-1g	1g	期货

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Accession#: P43153 (5) Accession#: Q46085 (8) Accession#:Q9X721 (8) colA (5) colG (8) colH (8) 胶原酶 (7)

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基本描述

产品名称	胶原酶 NB 5 来源于溶组织梭菌
英文别名	Collagenase I \| Collagenase Type II \| (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene \| microbial c
规格或纯度	EnzymoPure™, 无菌, ≥0.10 U/mg
生化机理	Clostridial collagenases are among the most efficient degraders of eukaryotic collagen known; saprophytes use collagen as a carbon source while pathogens additionally digest collagen to aid in host colonization. Has both tripeptidylcarboxypeptidase on Gly-X-Y and endopeptidase activities; the endopeptidase cuts within the triple helix region of collagen while tripeptidylcarboxypeptidase successively digests the exposed ends, thus clostridial collagenases can digest large sections of collagen. Catalytic activity: Digestion of native collagen in the triple helical region at Xaa-\|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'.
产品介绍	Collagenases from Clostridium histolyticum are proteolytic enzymes that cleave peptide bonds in the triple helical collagen molecules of human or animal tissue in situ. For this reason collagenases are widely used for isolation of various cell types by tissue dissociation. Collagenase NB 5 Sterile Grade is sterile according to European Pharmacopoeia. Collagenase NB 5 Sterile Grade is a crude collagenase that contains collagenolytic and additional enzymatic activities including clostripain and neutral protease. The balanced ratio of these activities ensures gentle and efficient tissue dissociation. Specification Collagenase activity: ≥0.10 U/mg (PZ acc. to Wünsch) Sterility: must comply Application Collagenase NB 5 Sterile Grade is suitable for dissociation of a broad variety of tissue types. Storage conditions Collagenase NB 5 Sterile Grade is available as a lyophilized powder. It should be stored at +2 to +8℃ in a dry environment. Under these conditions the product is stable until the minimum shelf life stated on the certificate of analysis if repeated opening and closing of the vial is avoided. For storage of solutions please refer to “Stock solution”. Instructions for use: General Collagenase NB 5 Sterile Grade is suitable for isolation of a broad variety of cells from human or animal tissues. Tissue types include adipose tissue, cartilage, skin, placenta, and umbilical cord tissue. It can also be applied in cell culture for passaging, e.g. of embryonic stem cells. Tissue dissociation Recommended starting concentrations for selected applications: Adipose tissue (human, rodent): 0.2 – 0.3 PZ U/ml Cartilage (human, rodent): 0.3 – 0.4 PZ U/ml In general, the appropriate collagenase concentration depends on tissue type and origin as well as on the isolation procedure. Collagenase activity is at an optimum at 37℃ and pH 7.4. Stock solution Collagenase NB 5 Sterile Grade dissolves at a concentration of up to 150 mg/ml in all buffers which are commonly used for cell isolation. The enzyme solution must be constantly stored on ice. Since collagenase and some of the secondary proteases depend on calcium, it is recommended to use a buffer with ≥ 2 mM Ca²⁺. Absolutely no calcium chelating agents (e.g. EDTA) should be present at all. Reconstituted Collagenase NB 5 Sterile Grade can be aliquoted and stored at -20 °C. Aliquots are stable for 1 year if repeated freezing and thawing is avoided. Collagenase NB 5 Sterile Grade is sterile according to Ph. Eur. Therefore, 0.22 µm filtration is not necessary if sterile equipment and buffers are used. If 0.22 µm filtration is required, filters with low protein-binding properties (e.g. cellulose acetate, PVDF, or PES) are recommended. Working solution To prepare a working solution, the stock solution is diluted with buffer to achieve the required collagenase concentration. The working solution must be constantly stored on ice until use. Inactivation and inhibitors The dissociation process can be reduced, e.g. by cooling down or dilution of the enzyme solution. Collagenase is reversibly inactivated at high pH values and irreversibly inactivated at low pH values. Inhibitors of collagenase include cysteine or chelating agents like EDTA. Important note Collagenase NB 5 Sterile Grade is intended for research use only.
生物活性	≥0.10 U/mg
种属	溶组织梭菌(Clostridium histolyticum)
Accession #	P43153,Q46085,Q9X721
来源	天然
预测分子量	70–120 kDa

关联靶点（人）

HT-29 (80576 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

MCF7 (126967 活性数据)

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活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

SK-MEL-5 (47095 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

A549 (127892 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

储存与运输

物理形态	冻干(Lyophilized)
浓度	≥0.10 U/mg
复溶	建议通过将胶原酶 NB 溶解在适当体积的缓冲液（例如 HBSS，PBS，Tris 或 Ringer 溶液）中制成储备溶液。配制好的酶应进行无菌过滤（如果不是无菌的话），分装并保存在-20℃下。我们的稳定性研究表明，如果避免反复冷冻和解冻，该酶在这些条件下可以维持稳定一年。
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	长期储存2-8℃（大于1年）；复溶后建议分装。
CAS编号和信息	9001-12-1
酶学委员会编号	3.4.24.3
单位定义	The hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25℃, pH 7.1.
分子类型	酶

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批号(Lot Number)	证书类型	货号
ZJ25F0826197	分析证书	C279009
ZJ25F0826196	分析证书	C279009

技术文档和文章

c279009_collagenase nb 5 sterile grade product manual.pdf

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引用文献

1. Jie Cao, Xiaoxuan Du, Hui Zhao, Chenhong Zhu, Chenchen Li, Xin Zhang, Libin Wei, Xue Ke. (2023) Sequentially degradable hydrogel-microsphere loaded with doxorubicin and pioglitazone synergistically inhibits cancer stemness of osteosarcoma. BIOMEDICINE & PHARMACOTHERAPY, 165 (115096). [PMID:37421781] [10.1016/j.biopha.2023.115096]
2. Meiqi Jin, Ningning Sun, Wenxian Weng, Zhentao Sang, Taotao Liu, Wei Xia, Shuze Wang, Xiaoting Sun, Tianlin Wang, Heran Li, Huazhe Yang. (2023) The effect of GelMA/alginate interpenetrating polymeric network hydrogel on the performance of porous zirconia matrix for bone regeneration applications. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 242 (124820). [PMID:37178890] [10.1016/j.ijbiomac.2023.124820]
3. Lanting Wei, Xiang Wang, Junjie Fu, Jian Yin, Jing Hu. (2023) A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 668 (131410). [10.1016/j.colsurfa.2023.131410]
4. Hongqian Zhang, Feng Ding, Zongwei Zhu, Qian Sun, Chuanxu Yang. (2023) Engineered ionizable lipid nanoparticles mediated efficient siRNA delivery to macrophages for anti-inflammatory treatment of acute liver injury. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 631 (122489). [PMID:36521639] [10.1016/j.ijpharm.2022.122489]
5. Zunhan Hu, Wenzhen Wu, Meizhe Yu, Zhi Wang, Zhenyu Yang, Xiaodong Xing, Xiaofang Chen, Lina Niu, Fan Yu, Yuhong Xiao, Jihua Chen. (2023) Mussel-inspired polymer with catechol and cationic Lys functionalities for dentin wet bonding. Materials Today Bio, 18 (100506). [PMID:36471892] [10.1016/j.mtbio.2022.100506]
6. Wanmei Zhou, Xueming Dong, Yang He, Wei Zheng, Jinsong Leng. (2022) In-vitro and in-vivo studies of 4D printed shape memory scaffolds with bioactive fillers and coating for enhanced bone tissue regeneration. Smart Materials and Structures, 31 (10): (105002). [10.1088/1361-665X/ac884a]
7. Qian Ji, Hao Zhang, Xihe Zhang, Qiaoqiao Ma, Liping Teng, Lipeng Qiu. (2020) Hydrosoluble collagen based biodegradable hybrid hydrogel for biomedical scaffold. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 31 (17): (2199-2219). [PMID:32663418] [10.1080/09205063.2020.1796229]
8. Guangxiu Cao, Cunyang Wang, Yubo Fan, Xiaoming Li. (2020) Biomimetic SIS-based biocomposites with improved biodegradability, antibacterial activity and angiogenesis for abdominal wall repair. Materials Science & Engineering C-Materials for Biological Applications, 109 (110538). [PMID:32228945] [10.1016/j.msec.2019.110538]
9. Dajiang Kuang, Fujian Jiang, Feng Wu, Kulwinder Kaur, Sourabh Ghosh, Subhas C. Kundu, Shenzhou Lu. (2019) Highly elastomeric photocurable silk hydrogels. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 134 (838). [PMID:31103592] [10.1016/j.ijbiomac.2019.05.068]
10. Huiyan Ying, Juan Zhou, Mingyu Wang, Dandan Su, Qiaoqiao Ma, Guozhong Lv, Jinghua Chen. (2019) In situ formed collagen-hyaluronic acid hydrogel as biomimetic dressing for promoting spontaneous wound healing. Materials Science & Engineering C-Materials for Biological Applications, 101 (487). [PMID:31029343] [10.1016/j.msec.2019.03.093]
11. Juan Zhou, Huiyan Ying, Mingyu Wang, Dandan Su, Guozhong Lu, Jinghua Chen. (2018) Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation. Materials Science & Engineering C-Materials for Biological Applications, 92 (447). [PMID:30184770] [10.1016/j.msec.2018.06.072]
12. Liuxin Yang, Fengya Jing, Dandan Wei, Xiaocong Zhao, Yinghua Tao, Tao Liu, Tianzhu Zhang. (2025) Assembled granular hydrogels loaded with growth factors for enhanced mesenchymal stem cell therapy in abdominal wall defect repair. JOURNAL OF CONTROLLED RELEASE, 381 (113630). [PMID:40090523] [10.1016/j.jconrel.2025.113630]
13. Tao Li, Yongtao Yan, Linlin Han, Mingkun Li, Shiyu Liu, XinYang Zhou, Jungjoon Lee, Xiaomin Li, Yuqing Zhao. (2024) Ginseng fruit rare saponins (GFRS), a promising anti-wrinkle agent: Evidence of its antioxidant effect and its capacity to prevent matrix metalloproteinase (MMPs) expression in vitro and in vivo. INDUSTRIAL CROPS AND PRODUCTS, 215 (118716). [10.1016/j.indcrop.2024.118716]
14. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li. (2024) Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound. ACS Nano, 18 (28): (18412-18424). [PMID:38949962] [10.1021/acsnano.4c02921]
15. Chen Zhengfang, He Mudan, Wang Houpeng, Li Xuehui, Qin Ruirui, Ye Ding, Zhai Xue, Zhu Junwen, Zhang Quanqing, Hu Peng, Shui Guanghou, Sun Yonghua. (2024) Intestinal DHA-PA-PG axis promotes digestive organ expansion by mediating usage of maternally deposited yolk lipids. Nature Communications, 15 (1): (1-15). [PMID:39528516] [10.1038/s41467-024-54258-2]
16. Liuxin Yang, Dandan Wei, Yinghua Tao, Fengya Jing, Tao Liu, Guanhua Jiao, Tianzhu Zhang. (2024) Spatiotemporal delivery of microenvironment responsive hydrogel incorporated with stem cells-loaded porous microspheres for abdominal wall repair. CHEMICAL ENGINEERING JOURNAL, 497 (154435). [10.1016/j.cej.2024.154435]
17. Liuxin Yang, Guanhua Jiao, Dandan Wei, Tianzhu Zhang. (2025) Synthesis and characterization of photocrosslinked gelatin-silk fibroin composite microspheres for mesenchymal stem cell expansion and osteogenic differentiation. Materials Today Communications, 44 (111912). [10.1016/j.mtcomm.2025.111912]

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