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胶原蛋白酶 来源于溶组织梭菌(类型4)

19篇引用文献 COA COA
  • Accession #: P06609
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货号 (SKU) 包装规格 是否现货 价格 数量
C128709-250mg
 250mg 期货 Stock Image
C128709-1g
 1g 期货 Stock Image
C128709-5g
 5g 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
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Amino Acid/Protein Metabolism (4559) Biochemical Assay Reagents (972)
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基本描述

产品名称 胶原蛋白酶 来源于溶组织梭菌(类型4)
别名 梭菌肽酶 A
英文别名 Collagenase I | Collagenase Type II | (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene
规格或纯度 EnzymoPure™, ≥160 units/mg dry weight
产品介绍

Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.
This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations.

Type 4 is designed to be especially low in tryptic activity to limit damage to membrane proteins and receptors.
Accession # P06609

关联靶点（人）

HT-29 (80576 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
MCF7 (126967 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
SK-MEL-5 (47095 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID
A549 (127892 活性数据)
活性类型 Relation Activity value Units Action Type 期刊 PubMed Id doi Assay Aladdin ID

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

储存与运输

浓度 ≥160 units/mg dry weight
储存温度 2-8°C储存
运输条件 冰袋运输
CAS编号和信息 9001-12-1
酶学委员会编号 I.U.B.: 3.4.24.3
单位定义 Unit Definition: One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5
分子类型 小分子

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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批号(Lot Number) 证书类型 货号
E2128235 分析证书 C128709
H2205028 分析证书 C128709
H2205029 分析证书 C128709
J2311022 分析证书 C128709
K2411052 分析证书 C128709
C2527302 分析证书 C128709
F23081027 分析证书 C128709

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