按主题分类的文章 "protein"

1. Principles of Enzymology and Its Biotechnological Applications
   发布于 2025年9月12日

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   Enzymes are highly efficient and specific biological catalysts, most of which are proteins. They can significantly accelerate the rate of biochemical reactions without altering the reaction equilibrium.
2. Does paraformaldehyde fixation disrupt cell membranes?
   发布于 2024年8月28日

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   Does paraformaldehyde fixation disrupt cell membranes?
3. Does Proteinase K powder need to be protected from light when storing and weighing?
   发布于 2024年8月28日

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   Does Proteinase K powder need to be protected from light when storing and weighing?
4. What solution should be used to dissolve Proteinase K when used? What is the common working concentration? At what degree does it need to be stored?
   发布于 2024年8月28日

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   What solution should be used to dissolve Proteinase K when used? What is the common working concentration? At what degree does it need to be stored?
5. Is this lysate suitable for extracting mitochondrial proteins?
   发布于 2024年8月28日

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   Is this lysate suitable for extracting mitochondrial proteins?
6. Do I need to add extra mercaptoethanol before using this product?
   发布于 2024年8月28日

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   Do I need to add extra mercaptoethanol before using this product?
7. Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?
   发布于 2024年8月28日

   1
   Protein samples boiled in buffer are blue in color and turn blue-violet after being left at -80°C. What is the reason for this? Does it affect the subsequent electrophoresis?
8. After the protein sample has been spiked with Sampling Buffer, boiled at 95°C for 10 min, and centrifuged at 14,000, individual samples will be delaminated prior to sampling, what is the reason for this?
   发布于 2024年8月28日

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   After the protein sample has been spiked with Sampling Buffer, boiled at 95°C for 10 min, and centrifuged at 14,000, individual samples will be delaminated prior to sampling, what is the reason for this?
9. BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
   发布于 2024年8月28日

   1
   BCA concentration measured protein concentration 3mg/ml, but after adding buffer found no protein bands appeared, what is the reason?
10. Is there a requirement for protein concentration for this product? If the protein concentration is high, do I need to increase the concentration of the buffer?
    发布于 2024年8月28日

    1
    Is there a requirement for protein concentration for this product? If the protein concentration is high, do I need to increase the concentration of the buffer?