Recombinant CD45 Antibody (AF405)

COA

级别和纯度:

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry

宿主种属: 大鼠(Rat)
种属反应性: 小鼠(Mouse)
亚型: Rat IgG2b
克隆号: P07H10
偶联: AF405

有货

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货号 (SKU)	包装规格	是否现货
Ab213882-10μg	10μg	现货
Ab213882-25μg	25μg	现货
Ab213882-100μg	100μg	现货

查看相关系列

点击查看靶蛋白信息: PTPRC

Accession#:P06800 (30) AF405 (97) Gene ID:19264 (30) Ly-5 (23)

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基本信息

产品名称	Recombinant CD45 Antibody (AF405)
别名	CD45抗体
英文别名	B220 antibody \| CD 45 antibody \| CD45 antibody \| CD45 antigen antibody \| CD45R antibody \| GP180 antibody \| L-CA antibody \| LCA antibody \| Leukocyte common antigen antibody \| loc antibody \| Ly-5 antibody \| LY5 antibody \| Ly5, homolog of antibody \| Lyt-4 an
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity.
宿主种属	大鼠(Rat)
特异性	Mouse CD45
种属反应性	小鼠(Mouse)
免疫原	Mouse thymus or spleen
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	AF405
Excitation(nm)	Violet Laser(405nm)
Emission(nm)	422nm
Ex/Em(nm)	Ex:401nm;Em:422nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

PTPRC Tchem 受体型酪氨酸蛋白磷酸酶C(Receptor-type tyrosine-protein phosphatase C) (0 活性数据)

点击查看靶蛋白信息： PTPRC

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD45 Antibody (AF405) (Ab213882)

Recombinant CD45 Antibody (AF405) (Ab213882) - Flow Cytometry
Flow cytometry analysis of CD45 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD45 Antibody (AF405) (Ab213882) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).

Flow Cytometry: Recombinant CD45 Antibody (AF405) (Ab213882)

Recombinant CD45 Antibody (AF405) (Ab213882) - Flow Cytometry
Flow cytometry analysis of CD45 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD45 Antibody (AF405) (Ab213882) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.