基本信息

产品名称	Recombinant c-Kit Antibody (AF647)
别名	原癌基因 \| 干细胞因子 \| 酪氨酸激酶受体 \| 干细胞因子受体 \| Anti-c-Kit抗体 \| c-Kit小鼠单克隆抗体 \| c-Kit抗体 \| KIT抗体 \| Anti-KIT抗体 \| KIT小鼠单克隆抗体 \| Anti-SCFR抗体 \| SCFR小鼠单克隆抗体 \| CD117抗体 \| Anti-CD117抗体
英文别名	C Kit antibody \| c-Kit antibody \| c-Kit Ligand antibody \| CD117 antibody \| Kit antibody \| Kit Ligand antibody \| KIT oncogene antibody \| KIT proto oncogene receptor tyrosine kinase antibody \| KIT_HUMAN antibody \| Mast cell growth factor receptor antibody \|
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
宿主种属	大鼠(Rat)
特异性	Mouse c-Kit
种属反应性	小鼠(Mouse)
免疫原	Mouse bone marrow mast cells
阳性对照	Flow Cytometry: Mouse bone marrow cells
偶联	AF647
Excitation(nm)	Red Laser(633nm)
Emission(nm)	668nm
Ex/Em(nm)	Ex:650nm;Em:668nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

KIT Tclin 肥大细胞/干细胞生长因子受体试剂盒(Mast/stem cell growth factor receptor Kit) (0 活性数据)

点击查看靶蛋白信息： KIT

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant c-Kit Antibody (AF647) (Ab213865)

Recombinant c-Kit Antibody (AF647) (Ab213865) - Flow Cytometry
Two-color flow cytometric analysis of c-Kit expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with CD45R Rat mAb (FITC) (Ab191667) and either Rat IgG (AF647) (Ab190071; Left panel) or Recombinant c-Kit Antibody (AF647) (Ab213865; Right panel) at 0.2μg/test.

应用

应用名称	稀释比例
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.