基本信息

产品名称	Recombinant c-Kit Antibody
别名	原癌基因 \| 干细胞因子 \| 酪氨酸激酶受体 \| 干细胞因子受体 \| Anti-c-Kit抗体 \| c-Kit小鼠单克隆抗体 \| c-Kit抗体 \| KIT抗体 \| Anti-KIT抗体 \| KIT小鼠单克隆抗体 \| Anti-SCFR抗体 \| SCFR小鼠单克隆抗体 \| CD117抗体 \| Anti-CD117抗体
英文别名	C Kit antibody \| c-Kit antibody \| c-Kit Ligand antibody \| CD117 antibody \| Kit antibody \| Kit Ligand antibody \| KIT oncogene antibody \| KIT proto oncogene receptor tyrosine kinase antibody \| KIT_HUMAN antibody \| Mast cell growth factor receptor antibody \|
规格或纯度	无动物源, 无载体, 重组, ExactAb™, 无叠氮钠, 已验证, 高性能, ≥95%(SDS-PAGE), 见COA
生化机理	Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
宿主种属	大鼠(Rat)
特异性	Mouse c-Kit
种属反应性	小鼠(Mouse)
免疫原	Mouse bone marrow mast cells
阳性对照	Flow Cytometry: Mouse bone marrow cells
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.4
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
分子类型	抗体

关联靶点（人）

KIT Tclin 肥大细胞/干细胞生长因子受体试剂盒(Mast/stem cell growth factor receptor Kit) (0 活性数据)

点击查看靶蛋白信息： KIT

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant c-Kit Antibody (Ab210970)

Recombinant c-Kit Antibody (Ab210970) - Flow Cytometry
Two-color flow cytometric analysis of c-Kit expression on mouse bone marrow. C57BL/6 mouse bone marrow cells were stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or Recombinant c-Kit Antibody (Ab210970; Right panel) at 1.0 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD45R versus c-Kit (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.