Recombinant CD146 Antibody (FITC)

COA

级别和纯度:

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry

宿主种属: 大鼠(Rat)
种属反应性: 小鼠(Mouse)
亚型: Rat IgG2a
克隆号: P09B03
偶联: FITC

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab213319-10μg	10μg	现货
Ab213319-25μg	25μg	现货
Ab213319-100μg	100μg	现货

查看相关系列

点击查看靶蛋白信息: MCAM

Accession#: Q8R2Y2 (4) FITC (209) Gene ID: 84004 (4) MUC18 (4)

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基本信息

产品名称	Recombinant CD146 Antibody (FITC)
别名	CD146抗体 \| 细胞表面糖蛋白Muc18抗体
英文别名	A32 antigen antibody \| CD 146 antibody \| CD146 antibody \| CD146 antigen antibody \| Cell surface glycoprotein MUC18 antibody \| Cell surface glycoprotein P1H12 antibody \| Gicerin antibody \| Mcam antibody \| Melanoma adhesion molecule antibody \| Melanoma asso
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity).
宿主种属	大鼠(Rat)
特异性	Mouse CD146
种属反应性	小鼠(Mouse)
免疫原	Endothelial cell line TME-3H3
阳性对照	Flow Cytometry: bEND.3 cells
偶联	FITC
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	517nm
Ex/Em(nm)	Ex:498nm;Em:517nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

MCAM Tbio 细胞表面糖蛋白 MUC18(Cell surface glycoprotein MUC18) (0 活性数据)

点击查看靶蛋白信息： MCAM

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD146 Antibody (FITC) (Ab213319)

Recombinant CD146 Antibody (FITC) (Ab213319) - Flow Cytometry
Flow cytometry analysis of CD146 expression by bEND.3 cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD146 Antibody (FITC) (Ab213319) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (FITC) (Ab180311). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.