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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab210151-10μl |
10μl |
现货 ![]() |
| |
| Ab210151-50μl |
50μL |
现货 ![]() |
| |
| Ab210151-100μl |
100μL |
现货 ![]() |
| |
| Ab210151-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Lamin A/C Mouse mAb |
|---|---|
| 别名 | 核纤层蛋白 | 核纤层蛋白A | 核纤层蛋白C | Anti- Lamin A/C抗体 | LMNA小鼠单克隆抗体 | Lamin A/C小鼠单克隆抗体 | Lamin A/C抗体;LMNA抗体 |
| 英文别名 | 70 kDa lamin antibody | Cardiomyopathy dilated 1A (autosomal dominant) antibody | CDCD1 antibody | CDDC antibody | CMD1A antibody | CMT2B1 antibody | EMD2 antibody | FPL antibody | FPLD antibody | FPLD2 antibody | HGPS antibody | IDC antibody | Lamin A an |
| 规格或纯度 | 无动物源, 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, ≥95%(SDS-PAGE), 1.0 mg/mL |
| 生化机理 | 层蛋白是核薄层的组成成分,核薄层是核内膜核质一侧的纤维层,被认为是核膜的框架,也可能与染色质相互作用。层蛋白 A 和层蛋白 C 以等量存在于哺乳动物的核薄层中。在核组装、染色质组织、核膜和端粒动态中发挥重要作用。 前维生素 A/C 可加速平滑肌细胞衰老。它的作用是破坏血管平滑肌细胞(VSMCs)的有丝分裂并诱导 DNA 损伤,导致有丝分裂失败、基因组不稳定和过早衰老。 |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | Lamin A |
| 种属反应性 | 人(Human),大鼠(Rat) |
| 免疫原 | Recombinant protein corresponding to Human Lamin A |
| 阳性对照 | WB: HeLa, A549, MCF7, THP-1, K562 and C6 whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 轻链亚型 | kappa |
| SDS-PAGE | 150.0 KDa |
| 纯化方法 | Protein A purified |
| 储存缓冲液 | PBS, pH 7.2 |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存(1-2 周)。在 -20°C 或 -80°C 下保存。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
Lamin A/C Mouse mAb (Ab210151) - Western Blot
All lanes: Lamin A/C Mouse Antibody (Ab210151) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 74, 65 kDa
Observed band size: 72, 65 kDa
Exposure time: 10.0 s
Lamin A/C Mouse mAb (Ab210151) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Lamin A/C Mouse mAb (Ab210151) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170217) was used as an isotype control (Blue).
Lamin A/C Mouse mAb (Ab210151) - ICC/IF
IF analysis of Lamin A/C (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Lamin A/C Mouse mAb (Ab210151) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC | 1/200 |
| WB | 1/20000 – 1/200000 |
| Flow Cytometry | 1/800 – 1/50000 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab210151 | |
| 分析证书 | Ab210151 | |
| 分析证书 | Ab210151 |
¥239.90
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