MRPL32 Antibody

COA

级别和纯度:

无载体

已验证

ExactAb™

1.0 mg/mL

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 绵羊(Sheep)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab191537-10μl	10μl	现货
Ab191537-50μl	50μL	现货
Ab191537-100μl	100μL	期货
Ab191537-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: MRPL32

Accession#: Q9BYC8 (1) Gene ID: 64983 (1) MRPL32 (1)

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基本信息

产品名称	MRPL32 Antibody
别名	线粒体核糖体蛋白L32抗体
英文别名	39S ribosomal protein L27 \| HSPC250 \| L27mt \| mitochondrial \| MRP-L27 \| mrpl27 \| RM27_HUMAN \| L32mt \| bL32m \| HSPC283 \| MRP-L32 \| bMRP-59b
规格或纯度	ExactAb™, Carrier Free, Validated, 1.0 mg/mL
生化机理	线粒体核糖体（mitoribosome）是一种大型核糖核蛋白复合体，负责在线粒体内合成蛋白质。翻译后：MRPL32 前体由 m-AAA 蛋白酶（由 AFG3L2 和 SPG7 组成）加工，该蛋白酶可裂解 N 端转运肽。m-AAA 蛋白酶的裂解作用发生在组装成大亚基之前，这是线粒体核糖体（mitoribosome）组装的重要步骤。m-AAA蛋白酶的正确处理依赖于MRPL32紧密折叠的C-末端结构域中的锌结合区：锌依赖性折叠阻止了从N-末端开始的降解，并触发成熟MRPL32的释放（相似）。
宿主种属	兔(Rabbit)
特异性	MRPL32
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),绵羊(Sheep)
免疫原	A KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MRPL32.
阳性对照	WB: A549, Jurkat, Raji, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue. IF/ICC: Hep G2 cells.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Immunogen affinity purified
储存缓冲液	0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol, 0.01% Sodium azide, pH 7.3.
防腐剂	0.01% Sodium azide
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（12 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

MRPL32 Tdark 39S 核糖体蛋白 L32，线粒体(39S ribosomal protein L32, mitochondrial) (0 活性数据)

点击查看靶蛋白信息： MRPL32

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: MRPL32 Antibody (Ab191537)

MRPL32 Antibody (Ab191537) - Western Blot
All lanes: MRPL32 Antibody (Ab191537) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 21 kDa
Observed band size: 22 kDa
Exposure time: 5.8 s

IHC: MRPL32 Antibody (Ab191537)

MRPL32 Antibody (Ab191537) - IHC
Immunohistochemical analysis of MRPL32 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with MRPL32 Antibody (Ab191537) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: MRPL32 Antibody (Ab191537)

MRPL32 Antibody (Ab191537) - IF/ICC
Immunofluorescent analysis of MRPL32 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the MRPL32 Antibody (Ab191537) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

应用