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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab179928-10μl |
10μl |
现货 ![]() |
| |
| Ab179928-50μl |
50μL |
现货 ![]() |
| |
| Ab179928-100μl |
100μL |
现货 ![]() |
| |
| Ab179928-1ml |
1ml |
现货 ![]() |
|
| 产品名称 | Vinculin Mouse mAb |
|---|---|
| 别名 | 黏着斑蛋白抗体 | 黏着斑蛋白 | Anti-vinculin 抗体 | 粘着斑蛋白小鼠mAb |
| 英文别名 | CMD1W antibody | CMH15 antibody | Epididymis luminal protein 114 antibody | HEL114 antibody | Metavinculin antibody | MV antibody | MVCL antibody | OTTHUMP00000019861 antibody | OTTHUMP00000019862 antibody | VCL antibody | VINC antibody | VINC_HUMAN antib |
| 规格或纯度 | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0 mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | Vinculin |
| 种属反应性 | 人(Human),小鼠(Mouse) |
| 免疫原 | Recombinant full length protein corresponding to Human Vinculin |
| 阳性对照 | WB: HeLa, HEK293, HepG2, A431 and NIH/3T3 whole cell lysates Flow: HeLa cells |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | AB04/5D4 |
| Format | Whole IgG |
| 亚型 | IgG1 |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, pH 7.2 |
| 防腐剂 | No |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 +4°C 下短期储存(1-2 周)。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
Vinculin Mouse mAb (Ab179928) - Western Blot
All lanes: Vinculin Antibody (Ab179928) at 0.5 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 124.0 kDa
Observed band size: 129.7 kDa
Exposure time: 5.7 s
Vinculin Mouse mAb (Ab179928) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Vinculin Mouse mAb (Ab179928) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).
| 应用名称 | 稀释比例 |
|---|---|
| WB | 1/1,000-1/20,000 ; Predicted molecular weight: 124 kDa |
| Flow Cytometry | 0.125 μg/mL-1.0 μg/mL |
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