Recombinant Ran Antibody

COA

级别和纯度:

重组

已验证

ExactAb™

1.2 mg/mL

应用:

IF/ICC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 小鼠(Mouse), 猴(Monkey)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab124509-10μl	10μl	现货
Ab124509-50μl	50μL	现货
Ab124509-100μl	100μL	期货
Ab124509-1ml	1ml	期货

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点击查看靶蛋白信息: RAN

Accession#:P62826 (1) Gene ID:5901 (1) RAN (1)

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基本信息

产品名称	Recombinant Ran Antibody
别名	Ran抗体 \| 雄激素受体相关蛋白24抗体 \| 雄激素受体相关蛋白24抗体 \| Ran抗体 \| 重组Ran抗体
英文别名	Androgen receptor associated protein 24 antibody \| Androgen receptor-associated protein 24 antibody \| ARA 24 antibody \| ARA24 antibody \| Gsp1 antibody \| GTP binding nuclear protein RAN antibody \| GTP-binding nuclear protein Ran antibody \| GTPase Ran antib
规格或纯度	ExactAb™, Validated, 重组, 1.2 mg/mL
宿主种属	兔(Rabbit)
特异性	Ran
种属反应性	人(Human),小鼠(Mouse),猴(Monkey)
免疫原	A synthetic peptide derived from human Ran (AA 191-204)
阳性对照	WB: Hep G2, HEK293, Raji, Jurkat, HeLa and RAW 264.7 cell lysates. ICC/IF: U87-MG cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂	0.01% Sodium azide
浓度	1.2 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

RAN Tchem GTP 结合核蛋白 Ran(GTP-binding nuclear protein Ran) (0 活性数据)

点击查看靶蛋白信息： RAN

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

RAN Tchem GTP-binding nuclear protein Ran (244 活性数据)

点击查看靶蛋白信息： RAN

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Recombinant Ran Antibody (Ab124509)

Recombinant Ran Antibody (Ab124509) - Western Blot
All lanes: Recombinant Ran Antibody (Ab124509) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 24 kDa
Observed band size: 26 kDa
Exposure time: 10.0 s

IF/ICC: Recombinant Ran Antibody (Ab124509)

Recombinant Ran Antibody (Ab124509) - IF/ICC
Immunocytochemistry analysis of Ran (green) in U87-MG using Recombinant Ran Antibody (Ab124509), and cells were counterstained with DAPI (blue).

应用

应用名称	稀释比例
IF/ICC	1/50-1/200
WB	1/500-1/2000

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F0404026	分析证书	Ab124509
ZJ24F0404025	分析证书	Ab124509

技术文档和文章

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

探索主题:

冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

引用文献

1. Hsiao PW, Lin DL, Nakao R, Chang C. (1999) The linkage of Kennedy's neuron disease to ARA24, the first identified androgen receptor polyglutamine region-associated coactivator.. J Biol Chem, 274 (29): (20229-34). [PMID:10400640]
2. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
3. Steggerda SM, Black BE, Paschal BM. Monoclonal antibodies to NTF2 inhibit nuclear protein import by preventing nuclear translocation of the GTPase Ran.. Mol Biol Cell, [PMID:10679025]
4. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330]
5. Klaeger S, Heinzlmeir S and Wilhelm M et al. (2017) The target landscape of clinical kinase drugs.. Science, 358 (6367): [PMID:29191878] [10.1126/science.aan4368]
6. Matsumoto, T T and Beach, D D.. (1991) Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase.. Cell, (26): [PMID:1855255]
7. Bischoff, F R FR and Ponstingl, H H.. (1991) Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1961752]
8. Drivas, G T GT, Shih, A A, Coutavas, E E, Rush, M G MG and D'Eustachio, P P.. (1990) Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line.. Molecular and cellular biology, [PMID:2108320]
9. Scheffzek, K K, Klebe, C C, Fritz-Wolf, K K, Kabsch, W W and Wittinghofer, A A.. (1995) Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form.. Nature, (23): [PMID:7885480]
10. Ren, M M and 9 more authors.. (1995) Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing.. Molecular and cellular biology, [PMID:7891706]
11. Ren, M M, Drivas, G G, D'Eustachio, P P and Rush, M G MG.. (1993) Ran/TC4: a small nuclear GTP-binding protein that regulates DNA synthesis.. The Journal of cell biology, [PMID:8421051]
12. Palacios, I I, Weis, K K, Klebe, C C, Mattaj, I W IW and Dingwall, C C.. (1996) RAN/TC4 mutants identify a common requirement for snRNP and protein import into the nucleus.. The Journal of cell biology, [PMID:8636225]
13. Moroianu, J J, Blobel, G G and Radu, A A.. (1996) Nuclear protein import: Ran-GTP dissociates the karyopherin alphabeta heterodimer by displacing alpha from an overlapping binding site on beta.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:8692944]
14. Görlich, D D, Panté, N N, Kutay, U U, Aebi, U U and Bischoff, F R FR.. (1996) Identification of different roles for RanGDP and RanGTP in nuclear protein import.. The EMBO journal, (15): [PMID:8896452]
15. Fornerod, M M, Ohno, M M, Yoshida, M M and Mattaj, I W IW.. (1997) CRM1 is an export receptor for leucine-rich nuclear export signals.. Cell, (19): [PMID:9323133]
16. Bischoff, F R FR and Görlich, D D.. (1997) RanBP1 is crucial for the release of RanGTP from importin beta-related nuclear transport factors.. FEBS letters, (15): [PMID:9428644]
17. Ribbeck, K K, Lipowsky, G G, Kent, H M HM, Stewart, M M and Görlich, D D.. (1998) NTF2 mediates nuclear import of Ran.. The EMBO journal, (16): [PMID:9822603]
18. Askjaer, P P, Jensen, T H TH, Nilsson, J J, Englmeier, L L and Kjems, J J.. (1998) The specificity of the CRM1-Rev nuclear export signal interaction is mediated by RanGTP.. The Journal of biological chemistry, (11): [PMID:9837918]
19. Vetter, I R IR, Nowak, C C, Nishimoto, T T, Kuhlmann, J J and Wittinghofer, A A.. (1999) Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport.. Nature, (4): [PMID:10078529]
20. Paraskeva, E E and 7 more authors.. (1999) CRM1-mediated recycling of snurportin 1 to the cytoplasm.. The Journal of cell biology, (19): [PMID:10209022]
21. Chook, Y M YM and Blobel, G G.. (1999) Structure of the nuclear transport complex karyopherin-beta2-Ran x GppNHp.. Nature, (20): [PMID:10353245]
22. Vetter, I R IR, Arndt, A A, Kutay, U U, Görlich, D D and Wittinghofer, A A.. (1999) Structural view of the Ran-Importin beta interaction at 2.3 A resolution.. Cell, (28): [PMID:10367892]
23. Carazo-Salas, R E RE and 5 more authors.. (1999) Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.. Nature, (8): [PMID:10408446]
24. Zhang, Q H QH and 20 more authors.. (2000) Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.. Genome research, [PMID:11042152]
25. Renault, L L, Kuhlmann, J J, Henkel, A A and Wittinghofer, A A.. (2001) Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1).. Cell, (20): [PMID:11336674]
26. Lindsay, M E ME, Holaska, J M JM, Welch, K K, Paschal, B M BM and Macara, I G IG.. (2001) Ran-binding protein 3 is a cofactor for Crm1-mediated nuclear protein export.. The Journal of cell biology, (25): [PMID:11425870]
27. Englmeier, L L and 5 more authors.. (2001) RanBP3 influences interactions between CRM1 and its nuclear protein export substrates.. EMBO reports, [PMID:11571268]
28. Seewald, Michael J MJ, Körner, Carolin C, Wittinghofer, Alfred A and Vetter, Ingrid R IR.. (2002) RanGAP mediates GTP hydrolysis without an arginine finger.. Nature, (7): [PMID:11832950]
29. Moore, William W, Zhang, Chuanmao C and Clarke, Paul R PR.. (2002) Targeting of RCC1 to chromosomes is required for proper mitotic spindle assembly in human cells.. Current biology : CB, (20): [PMID:12194828]
30. Haendeler, Judith J, Hoffmann, Jörg J, Brandes, Ralf P RP, Zeiher, Andreas M AM and Dimmeler, Stefanie S.. (2003) Hydrogen peroxide triggers nuclear export of telomerase reverse transcriptase via Src kinase family-dependent phosphorylation of tyrosine 707.. Molecular and cellular biology, [PMID:12808100]
31. Zou, Yonglong Y, Lim, Seunghwan S, Lee, Kangmoon K, Deng, Xiaobing X and Friedman, Eileen E.. (2003) Serine/threonine kinase Mirk/Dyrk1B is an inhibitor of epithelial cell migration and is negatively regulated by the Met adaptor Ran-binding protein M.. The Journal of biological chemistry, (5): [PMID:14500717]
32. Hakata, Yoshiyuki Y, Yamada, Masami M and Shida, Hisatoshi H.. (2003) A multifunctional domain in human CRM1 (exportin 1) mediates RanBP3 binding and multimerization of human T-cell leukemia virus type 1 Rex protein.. Molecular and cellular biology, [PMID:14612415]
33. Andersen, Jens S JS and 5 more authors.. (2003) Proteomic characterization of the human centrosome by protein correlation profiling.. Nature, (4): [PMID:14654843]
34. Wang, Dakun D, Li, Zaibo Z, Schoen, Susan R SR, Messing, Edward M EM and Wu, Guan G.. (2004) A novel MET-interacting protein shares high sequence similarity with RanBPM, but fails to stimulate MET-induced Ras/Erk signaling.. Biochemical and biophysical research communications, (9): [PMID:14684163]
35. Petosa, Carlo C and 9 more authors.. (2004) Architecture of CRM1/Exportin1 suggests how cooperativity is achieved during formation of a nuclear export complex.. Molecular cell, (3): [PMID:15574331]
36. Takeda, Eri E, Hieda, Miki M, Katahira, Jun J and Yoneda, Yoshihiro Y.. (2005) Phosphorylation of RanGAP1 stabilizes its interaction with Ran and RanBP1.. Cell structure and function, [PMID:16428860]
37. Chi, An A and 16 more authors.. (2006) Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.. Journal of proteome research, [PMID:17081065]
38. Yamashita, Daisuke D and 5 more authors.. (2007) Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding.. The Journal of biological chemistry, (9): [PMID:17209048]
39. Van Impe, Katrien K and 6 more authors.. (2008) A new role for nuclear transport factor 2 and Ran: nuclear import of CapG.. Traffic (Copenhagen, Denmark), [PMID:18266911]
40. Xia, Fang F, Canovas, Pedro M PM, Guadagno, Thomas M TM and Altieri, Dario C DC.. (2008) A survivin-ran complex regulates spindle formation in tumor cells.. Molecular and cellular biology, [PMID:18591255]
41. Schrader, Nils N and 6 more authors.. (2008) The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex.. Structure (London, England : 1993), [PMID:18611384]
42. Sanz-García, Marta M, López-Sánchez, Inmaculada I and Lazo, Pedro A PA.. (2008) Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities.. Molecular & cellular proteomics : MCP, [PMID:18617507]
43. Monecke, Thomas T and 5 more authors.. (2009) Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP.. Science (New York, N.Y.), (22): [PMID:19389996]
44. Medendorp, Klaas K and 7 more authors.. (2009) The mitotic arrest deficient protein MAD2B interacts with the small GTPase RAN throughout the cell cycle.. PloS one, (15): [PMID:19753112]
45. Koyama, Masako M and Matsuura, Yoshiyuki Y.. (2010) An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1.. The EMBO journal, (16): [PMID:20485264]
46. Bienvenut, Willy V WV and 6 more authors.. (2012) Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-α-acetylation features.. Molecular & cellular proteomics : MCP, [PMID:22223895]
47. Van Damme, Petra P and 15 more authors.. (2012) N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:22814378]
48. Taltynov, Oliver O and 8 more authors.. (2013) Interaction of transportin-SR2 with Ras-related nuclear protein (Ran) GTPase.. The Journal of biological chemistry, (30): [PMID:23878195]
49. Maertens, Goedele N GN and 13 more authors.. (2014) Structural basis for nuclear import of splicing factors by human Transportin 3.. Proceedings of the National Academy of Sciences of the United States of America, (18): [PMID:24449914]
50. Tsirkone, Vicky G VG and 5 more authors.. (2014) Structure of transportin SR2, a karyopherin involved in human disease, in complex with Ran.. Acta crystallographica. Section F, Structural biology communications, [PMID:24915079]
51. Bacot-Davis, Valjean R VR, Ciomperlik, Jessica J JJ, Basta, Holly A HA, Cornilescu, Claudia C CC and Palmenberg, Ann C AC.. (2014) Solution structures of Mengovirus Leader protein, its phosphorylated derivatives, and in complex with nuclear transport regulatory protein, RanGTPase.. Proceedings of the National Academy of Sciences of the United States of America, (4): [PMID:25331866]
52. Rudack, Till T and 5 more authors.. (2015) Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.. The Journal of biological chemistry, (2): [PMID:26272610]
53. Dörr, Angela A and 5 more authors.. (2015) MYCBP2 Is a Guanosine Exchange Factor for Ran Protein and Determines Its Localization in Neurons of Dorsal Root Ganglia.. The Journal of biological chemistry, (16): [PMID:26304119]
54. Fung, Ho Yee Joyce HY, Fu, Szu-Chin SC, Brautigam, Chad A CA and Chook, Yuh Min YM.. (2015) Structural determinants of nuclear export signal orientation in binding to exportin CRM1.. eLife, (8): [PMID:26349033]
55. Aksu, Metin M, Trakhanov, Sergei S and Görlich, Dirk D.. (2016) Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A.. Nature communications, (16): [PMID:27306458]
56. Fung, Ho Yee Joyce HY, Fu, Szu-Chin SC and Chook, Yuh Min YM.. (2017) Nuclear export receptor CRM1 recognizes diverse conformations in nuclear export signals.. eLife, (10): [PMID:28282025]
57. Bao, Xiaoling X and 19 more authors.. (2018) Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation.. Journal of molecular cell biology, (1): [PMID:29040603]
58. Piano Mortari, E E and 17 more authors.. (2018) The Vici syndrome protein EPG5 regulates intracellular nucleic acid trafficking linking autophagy to innate and adaptive immunity.. Autophagy, [PMID:29130391]

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体积（添加到小瓶中）

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所需的复溶浓度

Recombinant Ran Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

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