货号 (SKU)

包装规格

是否现货

价格

数量

Ab124215-10μl

10μl

现货

Ab124215-50μl

50μL

现货

Ab124215-100μl

100μL

期货

Ab124215-1ml

1ml

期货

基本信息

产品名称	Recombinant Rab4 Antibody
别名	癌基因RAS相关蛋白Rab4A抗体 \| 重组 Rab4 抗体
英文别名	HRES 1 / RAB4 \| Oncogene RAB4 \| OTTHUMP00000042824 \| Rab 4 \| RAB 4A \| RAB4 member RAS oncogene family \| RAB4A \| RAB4A member RAS oncogene family \| RAB4A_HUMAN \| Ras related protein Rab 4A \| Ras related protein Rab4A \| Ras-related protein Rab-4A \| HRES 1 /
规格或纯度	ExactAb™, Validated, 重组, 1.2 mg/mL
宿主种属	兔(Rabbit)
特异性	RAB4A
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	A synthetic peptide derived from human Rab4 (AA 50-75)
阳性对照	WB: MCF7, THP-1 and Hep G2 cell lysates. ICC/IF: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂	0.02% Sodium azide
浓度	1.2 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

RAB4A Tbio Ras相关蛋白Rab-4A(Ras-related protein Rab-4A) (0 活性数据)

点击查看靶蛋白信息： RAB4A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Recombinant Rab4 Antibody (Ab124215)

Recombinant Rab4 Antibody (Ab124215) - Western Blot
All lanes: Recombinant Rab4 Antibody (Ab124215) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution

Predicted band size: 24 kDa
Observed band size: 25 kDa
Exposure time: 36.7 s

IF/ICC: Recombinant Rab4 Antibody (Ab124215)

Recombinant Rab4 Antibody (Ab124215) - IF/ICC
Immunofluorescence analysis of Rab4 in HeLa using Recombinant Rab4 Antibody (Ab124215), and cells were counterstained with DAPI (blue).

应用

应用名称	稀释比例
WB	1/500-1/1000
IF/ICC	1/50-1/200

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F0505940	分析证书	Ab124215
ZJ24F0505941	分析证书	Ab124215

技术文档和文章

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

探索主题:

冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

引用文献

1. van der Sluijs, P P and 5 more authors.. (1992) Reversible phosphorylation--dephosphorylation determines the localization of rab4 during the cell cycle.. The EMBO journal, [PMID:1425574]
2. Bailly, E E and 5 more authors.. (1991) Phosphorylation of two small GTP-binding proteins of the Rab family by p34cdc2.. Nature, (25): [PMID:1902553]
3. Zahraoui, A A, Touchot, N N, Chardin, P P and Tavitian, A A.. (1989) The human Rab genes encode a family of GTP-binding proteins related to yeast YPT1 and SEC4 products involved in secretion.. The Journal of biological chemistry, (25): [PMID:2501306]
4. Nagelkerken, B B and 8 more authors.. (2000) Rabaptin4, a novel effector of the small GTPase rab4a, is recruited to perinuclear recycling vesicles.. The Biochemical journal, (15): [PMID:10698684]
5. Lindsay, Andrew J AJ and 6 more authors.. (2002) Rab coupling protein (RCP), a novel Rab4 and Rab11 effector protein.. The Journal of biological chemistry, (5): [PMID:11786538]
6. de Renzis, Stefano S, Sönnichsen, Birte B and Zerial, Marino M.. (2002) Divalent Rab effectors regulate the sub-compartmental organization and sorting of early endosomes.. Nature cell biology, [PMID:11788822]
7. Fouraux, Michael A MA and 8 more authors.. (2004) Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes.. Molecular biology of the cell, [PMID:14617813]
8. Lindsay, Andrew J AJ and McCaffrey, Mary W MW.. (2004) Characterisation of the Rab binding properties of Rab coupling protein (RCP) by site-directed mutagenesis.. FEBS letters, (30): [PMID:15280022]
9. Huber, Silke K SK and Scheidig, Axel J AJ.. (2005) High resolution crystal structures of human Rab4a in its active and inactive conformations.. FEBS letters, (23): [PMID:15907487]
10. Eathiraj, Sudharshan S, Pan, Xiaojing X, Ritacco, Christopher C and Lambright, David G DG.. (2005) Structural basis of family-wide Rab GTPase recognition by rabenosyn-5.. Nature, (21): [PMID:16034420]
11. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
12. Nagy, Gyorgy G and 12 more authors.. (2006) Regulation of CD4 expression via recycling by HRES-1/RAB4 controls susceptibility to HIV infection.. The Journal of biological chemistry, (10): [PMID:16935861]
13. Kachhap, Sushant K SK and 8 more authors.. (2007) The N-Myc down regulated Gene1 (NDRG1) Is a Rab4a effector involved in vesicular recycling of E-cadherin.. PloS one, (5): [PMID:17786215]
14. Hoogenraad, Casper C CC and 17 more authors.. (2010) Neuron specific Rab4 effector GRASP-1 coordinates membrane specialization and maturation of recycling endosomes.. PLoS biology, (19): [PMID:20098723]

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稀释计算器

计算配制储备溶液所需的稀释度。

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复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Rab4 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器

Recombinant Rab4 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器