JavaScript 似乎在您的浏览器中禁用。 在我们的网站上获得最佳体验，请务必打开 Javascript 在您的浏览器。

This is a demo store. No orders will be fulfilled.

菜单

设置

྾

首页
抗体
单克隆抗体
Rab20 Mouse mAb

Rab20 Mouse mAb

级别和纯度:

0.5 mg/mL

应用:

Flow Cytometry
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 小鼠(Mouse)
亚型: Mouse IgG2a
克隆号: 1694CT210.218.54
偶联: Unconjugated

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab124145-10μl	10μl	现货
Ab124145-50μl	50μL	现货
Ab124145-100μl	100μL	期货
Ab124145-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: RAB20

Accession#: Q9NX57 (1) Gene ID: 55647 (1) RAB20 (1) 流式细胞术 (1163)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本信息

产品名称	Rab20 Mouse mAb
别名	Rab20抗体 \| Rab20小鼠单克隆抗体 \| Rab20小鼠单抗
英文别名	FLJ20429 antibody \| RAB20 antibody \| RAB20 member RAS oncogene family antibody \| RAB20_HUMAN antibody \| Ras related protein Rab20 antibody \| Ras-related protein Rab-20 antibody
规格或纯度	无载体, ExactAb™, 高性能, 已验证, 0.5 mg/mL
生化机理	Plays a role in apical endocytosis/recycling. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes.
宿主种属	小鼠(Mouse)
特异性	Rab20
种属反应性	人(Human),小鼠(Mouse)
免疫原	Recombinant protein of human RAB20 (AA 1-190)
阳性对照	WB: THP-1, K562 and MCF7 cell lysates. Flow: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
亚型	Mouse IgG2a
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 0.09% Sodium azide, pH 7.4
防腐剂	0.09% Sodium azide
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

RAB20 Tbio Ras 相关蛋白 Rab-20(Ras-related protein Rab-20) (0 活性数据)

点击查看靶蛋白信息： RAB20

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Rab20 Mouse mAb (Ab124145)

Rab20 Mouse mAb (Ab124145) - Western Blot
All lanes: Rab20 Mouse mAb (Ab124145) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti Mouse HRP (Ab179001) at 1/10000 dilution

Predicted band size: 26 kDa
Observed band size: 29 kDa
Exposure time: 60.0 s

Flow Cytometry: Rab20 Mouse mAb (Ab124145)

Rab20 Mouse mAb (Ab124145) - Flow Cytometry
Overlay histogram showing HeLa cells stained with Rab20 Mouse mAb (Ab124145) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Rab20 Mouse mAb (Ab124145) (1/25 dilution) for 60 min at 37 ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37 ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

应用

应用名称	稀释比例
Flow Cytometry	1/25
WB	1/500-1/4000

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ24F1012606	分析证书	Ab124145
ZJ24F1012605	分析证书	Ab124145

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection