Recombinant Pin1 Antibody

COA

级别和纯度:

重组

已验证

ExactAb™

见COA

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 仓鼠(Hamster), 大鼠(Rat), 小鼠(Mouse)
亚型: Rabbit IgG
偶联: Unconjugated

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货号 (SKU)	包装规格	是否现货
Ab121891-10μl	10μl	现货
Ab121891-50μl	50μL	现货
Ab121891-100μl	100μL	现货
Ab121891-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: PIN1

Accession#: Q13526 (1) Gene ID: 5300 (1) PIN1 (1)

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基本信息

产品名称	Recombinant Pin1 Antibody
别名	肽基脯氨酞顺反异构酶抗体 \| 肽基脯氨酞顺反异构酶单克隆抗体 \| 肽基脯氨酞顺反异构酶重组单克隆抗体 \| Pin1抗体 \| Pin1单克隆抗体 \| Pin1重组单克隆抗体
英文别名	EC 5.2.1.8 \| Peptidyl-prolyl cis-trans isomerase Pin1 \| PPIase Pin1 \| Rotamase Pin1 \| DOD antibody \| DODO, Drosophila, homolog of antibody \| FLJ40239 antibody \| FLJ77628 antibody \| MGC10717 antibody \| NIMA interacting 1 antibody \| Peptidyl prolyl cis tran
规格或纯度	重组, ExactAb™, 已验证, 见COA
生化机理	Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. Post-translational: Phosphorylated upon DNA damage, probably by ATM or ATR.
宿主种属	兔(Rabbit)
特异性	PIN1
种属反应性	人(Human),仓鼠(Hamster),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant protein of human Pin1 (AA 1-165)
阳性对照	WB: Jurkat, HeLa, MCF7, Raji, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue. ICC/IF: HEK293 cells.
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Rabbit IgG
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂	0.01% Sodium azide
浓度	见COA
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
分子类型	抗体

关联靶点（人）

PIN1 Tchem 肽基脯氨酰顺反异构酶NIMA相互作用1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) (0 活性数据)

点击查看靶蛋白信息： PIN1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Recombinant Pin1 Antibody

Recombinant Pin1 Antibody (Ab121891) - Western Blot
All lanes: Recombinant Pin1 Antibody (Ab121891) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 18 kDa
Observed band size: 17 kDa
Exposure time: 14.3 s

IF/ICC: Recombinant Pin1 Antibody (Ab121891)

Recombinant Pin1 Antibody (Ab121891) - IF/ICC
Immunocytochemistry analysis of PIN1 (green) in 293 using Recombinant Pin1 Antibody (Ab121891), and DAPI (blue).

IHC: Recombinant Pin1 Antibody (Ab121891)

Recombinant Pin1 Antibody (Ab121891) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Pin1 Antibody (Ab121891). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

应用

应用名称	稀释比例
WB	1/500-1/1000
IHC	1/50 - 1/100
IF/ICC	1/50-1/200

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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到5个结果

批号(Lot Number)	证书类型	货号
ZJ25F0723605	分析证书	Ab121891
ZJ25F0723606	分析证书	Ab121891
ZJ25F0723607	分析证书	Ab121891
ZJ24F0506151	分析证书	Ab121891
ZJ24F0506150	分析证书	Ab121891

技术文档和文章

免疫荧光染色实验步骤（甲醇通透法）

Categories: 实验方案（Protocols）

探索主题:

冷冻切片免疫荧光染色组织切片甲醇通透 IF

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

Immunofluorescence Staining Protocol (Methanol permeabilization)

Categories: 实验方案（Protocols）

探索主题:

Frozen section IF Immunofluorescence staining Tissue section Methanol permeability

引用文献

1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861]
2. Nechama M, Kwon J, Wei S, Kyi AT, Welner RS, Ben-Dov IZ, Arredouani MS, Asara JM, Chen CH, Tsai CY et al.. (2018) The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation.. Nat Commun, 9 (1): (1603). [PMID:29686383]
3. Liu T, Liu Y, Kao HY, Pei D.. (2010) Membrane permeable cyclic peptidyl inhibitors against human Peptidylprolyl Isomerase Pin1.. J Med Chem, 53 (6): (2494-2501). [PMID:20180533] [10.1021/jm901778v]
4. Jiang B, Pei D.. (2015) A Selective, Cell-Permeable Nonphosphorylated Bicyclic Peptidyl Inhibitor against Peptidyl-Prolyl Isomerase Pin1.. J Med Chem, 58 (15): (6306-6312). [PMID:26196061] [10.1021/acs.jmedchem.5b00411]
5. Li K, Li L, Wang S, Li X, Ma T, Liu D, Jing Y, Zhao L.. (2017) Design and synthesis of novel 2-substituted 11-keto-boswellic acid heterocyclic derivatives as anti-prostate cancer agents with Pin1 inhibition ability.. Eur J Med Chem, 126 (910-919). [PMID:27997878] [10.1016/j.ejmech.2016.09.089]
6. Li K, Ma T, Cai J, Huang M, Guo H, Zhou D, Luan S, Yang J, Liu D, Jing Y, Zhao L.. (2017) Conjugates of 18β-glycyrrhetinic acid derivatives with 3-(1H-benzo[d]imidazol-2-yl)propanoic acid as Pin1 inhibitors displaying anti-prostate cancer ability.. Bioorg Med Chem, 25 (20): (5441-5451). [PMID:28838831] [10.1016/j.bmc.2017.08.002]
7. Huang M, Li A, Zhao F, Xie X, Li K, Jing Y, Liu D, Zhao L.. (2018) Design, synthesis and biological evaluation of ring A modified 11-keto-boswellic acid derivatives as Pin1 inhibitors with remarkable anti-prostate cancer activity.. Bioorg Med Chem Lett, 28 (19): (3187-3193). [PMID:30153964] [10.1016/j.bmcl.2018.08.021]
8. Fan X, He H, Li J, Luo G, Zheng Y, Zhou JK, He J, Pu W, Zhao Y.. (2019) Discovery of 4,6-bis(benzyloxy)-3-phenylbenzofuran as a novel Pin1 inhibitor to suppress hepatocellular carcinoma via upregulating microRNA biogenesis.. Bioorg Med Chem, 27 (11): (2235-2244). [PMID:31027708] [10.1016/j.bmc.2019.04.028]
9. Gour R, Ahmad F, Prajapati SK, Giri SK, Lal Karna SK, Kartha KPR, Pokharel YR.. (2019) Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity.. Eur J Med Chem, 178 (552-570). [PMID:31216504] [10.1016/j.ejmech.2019.06.018]
10. Ieda N, Itoh K, Inoue Y, Izumiya Y, Kawaguchi M, Miyata N, Nakagawa H.. (2019) An irreversible inhibitor of peptidyl-prolyl cis/trans isomerase Pin1 and evaluation of cytotoxicity.. Bioorg Med Chem Lett, 29 (3): (353-356). [PMID:30585173] [10.1016/j.bmcl.2018.12.044]
11. Lu, K P KP, Hanes, S D SD and Hunter, T T.. (1996) A human peptidyl-prolyl isomerase essential for regulation of mitosis.. Nature, (11): [PMID:8606777]
12. Ranganathan, R R, Lu, K P KP, Hunter, T T and Noel, J P JP.. (1997) Structural and functional analysis of the mitotic rotamase Pin1 suggests substrate recognition is phosphorylation dependent.. Cell, (13): [PMID:9200606]
13. Kamimoto, T T, Zama, T T, Aoki, R R, Muro, Y Y and Hagiwara, M M.. (2001) Identification of a novel kinesin-related protein, KRMP1, as a target for mitotic peptidyl-prolyl isomerase Pin1.. The Journal of biological chemistry, (5): [PMID:11470801]
14. Dougherty, Michele K MK and 9 more authors.. (2005) Regulation of Raf-1 by direct feedback phosphorylation.. Molecular cell, (21): [PMID:15664191]
15. Chen, Jian J and 7 more authors.. (2006) Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients.. Biochemical and biophysical research communications, (24): [PMID:16476580]
16. Yu, Liang L and 6 more authors.. (2006) Regulation of Bruton tyrosine kinase by the peptidylprolyl isomerase Pin1.. The Journal of biological chemistry, (30): [PMID:16644721]
17. Matsuoka, Shuhei S and 12 more authors.. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.. Science (New York, N.Y.), (25): [PMID:17525332]
18. Phan, Ryan T RT, Saito, Masumichi M, Kitagawa, Yukiko Y, Means, Anthony R AR and Dalla-Favera, Riccardo R.. (2007) Genotoxic stress regulates expression of the proto-oncogene Bcl6 in germinal center B cells.. Nature immunology, [PMID:17828269]
19. Buschdorf, Jan Paul JP, Chew, Li Li LL, Soh, Unice Jim Kim UJ, Liou, Yih-Cherng YC and Low, Boon Chuan BC.. (2008) Nerve growth factor stimulates interaction of Cayman ataxia protein BNIP-H/Caytaxin with peptidyl-prolyl isomerase Pin1 in differentiating neurons.. PloS one, (16): [PMID:18628984]
20. Lee, Tae Ho TH and 10 more authors.. (2011) Death-associated protein kinase 1 phosphorylates Pin1 and inhibits its prolyl isomerase activity and cellular function.. Molecular cell, (22): [PMID:21497122]
21. Lim, Jun Hee JH, Liu, Yu Y, Reineke, Erin E and Kao, Hung-Ying HY.. (2011) Mitogen-activated protein kinase extracellular signal-regulated kinase 2 phosphorylates and promotes Pin1 protein-dependent promyelocytic leukemia protein turnover.. The Journal of biological chemistry, (30): [PMID:22033920]
22. Min, Sang-Hyun SH and 16 more authors.. (2012) Negative regulation of the stability and tumor suppressor function of Fbw7 by the Pin1 prolyl isomerase.. Molecular cell, (29): [PMID:22608923]
23. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
24. Steger, Martin M and 13 more authors.. (2013) Prolyl isomerase PIN1 regulates DNA double-strand break repair by counteracting DNA end resection.. Molecular cell, (9): [PMID:23623683]
25. Ferretti, Lorenza P LP and 11 more authors.. (2016) Cullin3-KLHL15 ubiquitin ligase mediates CtIP protein turnover to fine-tune DNA-end resection.. Nature communications, (26): [PMID:27561354]

溶液计算器

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质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

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体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Pin1 Antibody

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器