MEK4/MKK4 Antibody

COA

级别和纯度:

无载体

已验证

ExactAb™

1.0 mg/mL

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 斑马鱼(Zebrafish), 猴(Monkey)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab114888-10μl	10μl	现货
Ab114888-50μl	50μL	现货
Ab114888-100μl	100μL	期货
Ab114888-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: MAP2K4

Accession#: P45985 (1) Gene ID: 6416 (1) MAP2K4 (1) MEK4 (1) MKK4 (1)

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基本信息

产品名称	MEK4/MKK4 Antibody
别名	nti-MEK4/MKK4抗体 \| MEK4/MKK4抗体 \| MEK4/MKK4 抗体
英文别名	c-Jun N-terminal kinase kinase 1 \| dual specificity mitogen-activated protein kinase kinase 4 \| JNK activating kinase 1 \| JNK-activated kinase 1 \| JNK-activating kinase 1 \| JNKK \| JNKK1 \| JNKK1MAPK/ERK kinase 4 \| MAP kinase kinase 4 \| MAP2K4 \| MAPKK4 \| ME
规格或纯度	ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属	兔(Rabbit)
特异性	MAP2K4
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),斑马鱼(Zebrafish),猴(Monkey)
免疫原	A KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MKK4
阳性对照	WB: HeLa, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human skeletal muscle tissue. IF/ICC: COS7 cells.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Immunogen affinity purified
物理外观	Liquid
储存缓冲液	0.42% Potassium phosphate, 0.87% Sodium chloride, 30% glycerol, and 0.01% sodium azide, PH 7.3
防腐剂	0.01% Sodium azide
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4°C 短期储存（1-2 周）。-20°C下长期保存（12个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

MAP2K4 Tchem 双特异性丝裂原活化蛋白激酶激酶 4(Dual specificity mitogen-activated protein kinase kinase 4) (0 活性数据)

点击查看靶蛋白信息： MAP2K4

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: MEK4/MKK4 Antibody (Ab114888)

MEK4/MKK4 Antibody (Ab114888) - Western Blot
All lanes: MEK4/MKK4 Antibody (Ab114888) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 46 kDa
Exposure time: 17.4 s

IF/ICC: MEK4/MKK4 Antibody (Ab114888)

MEK4/MKK4 Antibody (Ab114888) - IF/ICC
Immunofluorescent analysis of MEK4/MKK4 Antibody (Ab114888) staining in COS7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton
X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS
for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

IHC: MEK4/MKK4 Antibody (Ab114888)

MEK4/MKK4 Antibody (Ab114888) - IHC
Immunohistochemical analysis of MEK4/MKK4 Antibody (Ab114888) staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.