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Histone H3 (tri methyl K4) Antibody

Histone H3 (tri methyl K4) Antibody

级别和纯度:

1.0 mg/mL

应用:

ELISA
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 斑马鱼(Zebrafish), 牛(Cow), 猪(Pig), 鸡(Chicken)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab107957-10μl	10μl	现货
Ab107957-50μl	50μL	现货
Ab107957-100μl	100μL	现货
Ab107957-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: HIST1H3A

Accession#: Q6NXT2 (1) Accession#: Q71DI3 (1) Accession#:P68431 (4) Accession#:P84243 (2) Gene ID: 126961 (1) Gene ID: 3021 (1) Gene ID: 333932 (1) Gene ID: 440093 (1) Gene ID: 653604 (1) Gene ID: 8351 (1) Gene ID: 8352 (1) Gene ID: 8353 (1) Gene ID: 8354 (1) Gene ID: 8355 (1) Gene ID: 8356 (1) Gene ID: 8357 (1) Gene ID: 8358 (1) Gene ID: 8968 (1) Gene ID:3020 (2) Gene ID:8350 (4) H3-3A (2) H3-3B (1) H3-5 (1) H3C1 (4) H3C10 (1) H3C11 (1) H3C12 (1) H3C13 (1) H3C14 (1) H3C15 (1) H3C3 (1) H3C4 (1) H3C6 (1) H3C7 (1) H3C8 (1) Histone H3 (4)

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基本信息

产品名称	Histone H3 (tri methyl K4) Antibody
别名	三甲基组蛋白H3（Lys4）抗体 \| 三甲基组蛋白H3（Lys4）多抗 \| 三甲基组蛋白H3（Lys4）多克隆抗体
英文别名	H3 histone family member E pseudogene antibody \| H3 histone family, member A antibody \| H3/A antibody \| H31_HUMAN antibody \| H3F3 antibody \| H3FA antibody \| Hist1h3a antibody \| HIST1H3B antibody \| HIST1H3C antibody \| HIST1H3D antibody \| HIST1H3E antibody
规格或纯度	无载体, ExactAb™, 已验证, 1.0 mg/mL
生化机理	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
宿主种属	兔(Rabbit)
特异性	Histone H3 (tri methyl K4)
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),斑马鱼(Zebrafish),牛(Cow),猪(Pig),鸡(Chicken)
免疫原	KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K4 of human Histone H3 protein (AA 2-32).
阳性对照	WB: Mouse skeletal muscle, Rat skeletal muscle, HeLa, A549, K562 and Jurkat lysates. IHC: Human breast cancer tissue.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
SDS-PAGE	150 kDa
纯化方法	Immunogen affinity purified
物理外观	Liquid
储存缓冲液	0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol, 0.01% Sodium azide, pH 7.3
防腐剂	0.01% Sodium azide
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
分子类型	抗体

关联靶点（人）

HIST1H3A Tbio 组蛋白 H3.1(Histone H3.1) (0 活性数据)

点击查看靶蛋白信息： HIST1H3A

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Histone H3 (tri methyl K4) Antibody (Ab107957)

Histone H3 (tri methyl K4) Antibody (Ab107957) - Western Blot
All lanes: Histone H3 (tri methyl K4) Antibody (Ab107957) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 17 kDa
Exposure time: 25.4 s

Western Blot: Histone H3 (tri methyl K4) Antibody (Ab107957)

IHC: Histone H3 (tri methyl K4) Antibody (Ab107957)

Histone H3 (tri methyl K4) Antibody (Ab107957) - IHC
Immunohistochemical analysis of Histone H3 (TriMethyl-K4) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Histone H3 (tri methyl K4) Antibody (Ab107957) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ELISA: Histone H3 (tri methyl K4) Antibody (Ab107957)

Histone H3 (tri methyl K4) Antibody (Ab107957) - ELISA
Direct ELISA antibody dose-response curve using Histone H3 (tri methyl K4) Antibody (Ab107957). Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 µg/mL. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

应用