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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab105776-10μl |
10μl |
现货 ![]() |
| |
| Ab105776-50μl |
50μL |
现货 ![]() |
| |
| Ab105776-100μl |
100μL |
期货 ![]() |
| |
| Ab105776-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Recombinant Glycogen synthase 1/GYS1 Antibody |
|---|---|
| 别名 | 葡萄糖合成酶1抗体 | 重组糖原合酶1/GYS1抗体 |
| 英文别名 | Glycogen synthase 1 (muscle) | GSY | GYS | GYS1 | EC 2.4.1.11 | Glycogen synthase1 | GYS 1 | Starchsynthase muscle | UDP glucose glycogen glucosyltransferase | GYS1_HUMAN | Glycogen [starch] synthase, muscl | Glycogen [starch] synthase antibody | Glycogen |
| 规格或纯度 | ExactAb™, Validated, 重组, 0.7 mg/mL |
| 宿主种属 | 兔(Rabbit) |
| 特异性 | GYS1 |
| 种属反应性 | 人(Human),大鼠(Rat) |
| 免疫原 | A synthetic peptide derived from human Glycogen synthase 1 (AA 719-737) |
| 阳性对照 | WB: HEK293 and A431 cell lysates. ICC/IF: HeLa cells. |
| 偶联 | Unconjugated |
| 克隆类型 | 重组抗体 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Immunogen affinity purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4 |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 0.7 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
Recombinant Glycogen synthase 1/GYS1 Antibody (Ab105776) - Western Blot
All lanes: Recombinant Glycogen synthase 1/GYS1 Antibody (Ab105776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 84 kDa
Observed band size: 86 kDa
Exposure time: 84.3 s
Recombinant Glycogen synthase 1/GYS1 Antibody (Ab105776) - IF/ICC
Immunocytochemistry analysis of Glycogen synthase 1 (green) in HeLa using Recombinant Glycogen synthase 1/GYS1 Antibody (Ab105776), and cells were counterstained with DAPI (blue).
| 应用名称 | 稀释比例 |
|---|---|
| WB | 1/500-1/1000 |
| IF/ICC | 1/50-1/200 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab105776 | |
| 分析证书 | Ab105776 |
| 1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed. (2009-05-06) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.. Analytical chemistry, 81 ((11)): (4493-4501). [PMID:19413330] |
| 2. Browner, M F MF, Nakano, K K, Bang, A G AG and Fletterick, R J RJ.. (1989) Human muscle glycogen synthase cDNA sequence: a negatively charged protein with an asymmetric charge distribution.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2493642] |
| 3. Orho, M M, Nikula-Ijäs, P P, Schalin-Jäntti, C C, Permutt, M A MA and Groop, L C LC.. (1995) Isolation and characterization of the human muscle glycogen synthase gene.. Diabetes, [PMID:7657035] |
| 4. Su, X X, Schuler, L L and Shapiro, S S.. (1996) Cloning and characterization of a glycogen synthase cDNA from human endometrium.. The Journal of steroid biochemistry and molecular biology, [PMID:9010351] |
| 5. Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1): [PMID:15057824] |
| 6. Skurat, Alexander V AV, Dietrich, Amy D AD and Roach, Peter J PJ.. (2006) Interaction between glycogenin and glycogen synthase.. Archives of biochemistry and biophysics, (1): [PMID:17055998] |
| 7. Kollberg, Gittan G and 7 more authors.. (2007) Cardiomyopathy and exercise intolerance in muscle glycogen storage disease 0.. The New England journal of medicine, (11): [PMID:17928598] |
| 8. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648] |
| 9. Daub, Henrik H and 9 more authors.. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.. Molecular cell, (8): [PMID:18691976] |
| 10. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332] |
| 11. Rigbolt, Kristoffer T G KT and 9 more authors.. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.. Science signaling, (15): [PMID:21406692] |
| 12. Kelsall, Ian R IR, Voss, Martin M, Munro, Shonagh S, Cuthbertson, Daniel J R DJ and Cohen, Patricia T W PT.. (2011) R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals.. Journal of neurochemistry, [PMID:21668450] |
| 13. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163] |
| 14. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460] |