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Recombinant Glycogen synthase 1/GYS1 Antibody

    应用:
  • IF/ICC
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 人(Human), 大鼠(Rat)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab105776-10μl
10μl 现货 Stock Image
Ab105776-50μl
50μL 现货 Stock Image
Ab105776-100μl
100μL 期货 Stock Image
Ab105776-1ml
1ml 期货 Stock Image

基本信息

产品名称 Recombinant Glycogen synthase 1/GYS1 Antibody
别名 葡萄糖合成酶1抗体 | 重组糖原合酶1/GYS1抗体
英文别名 Glycogen synthase 1 (muscle) | GSY | GYS | GYS1 | EC 2.4.1.11 | Glycogen synthase1 | GYS 1 | Starchsynthase muscle | UDP glucose glycogen glucosyltransferase | GYS1_HUMAN | Glycogen [starch] synthase, muscl | Glycogen [starch] synthase antibody | Glycogen
规格或纯度 ExactAb™, Validated, 重组, 0.7 mg/mL
宿主种属 兔(Rabbit)
特异性 GYS1
种属反应性 人(Human),大鼠(Rat)
免疫原 A synthetic peptide derived from human Glycogen synthase 1 (AA 719-737)
阳性对照 WB: HEK293 and A431 cell lysates. ICC/IF: HeLa cells.
偶联 Unconjugated

产品属性

克隆类型 重组抗体
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Immunogen affinity purified
物理外观 Liquid
储存缓冲液 50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂 0.01% Sodium azide
浓度 0.7 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

关联靶点(人)

GYS1 Tchem 糖原[淀粉]合酶,肌肉(Glycogen [starch] synthase, muscle) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

作用机制

作用机制 Action Type target ID Target Name Target Type Target Organism Binding Site Name 参考文献

图片

Recombinant Glycogen synthase 1/GYS1 Antibody (Ab105776) - Western Blot
All lanes: Recombinant Glycogen synthase 1/GYS1 Antibody (Ab105776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 84 kDa
Observed band size: 86 kDa
Exposure time: 84.3 s

Recombinant Glycogen synthase 1/GYS1 Antibody (Ab105776) - IF/ICC
Immunocytochemistry analysis of Glycogen synthase 1 (green) in HeLa using Recombinant Glycogen synthase 1/GYS1 Antibody (Ab105776), and cells were counterstained with DAPI (blue).

应用

应用名称 稀释比例
WB

1/500-1/1000

IF/ICC

1/50-1/200

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批号(Lot Number) 证书类型 货号
ZJ24F0202778 分析证书 Ab105776
ZJ24F0202779 分析证书 Ab105776

引用文献

1. Sharon Gauci,Andreas O Helbig,Monique Slijper,Jeroen Krijgsveld,Albert J R Heck,Shabaz Mohammed.  (2009-05-06)  Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach..  Analytical chemistry,  81  ((11)): (4493-4501).  [PMID:19413330]
2. Browner, M F MF, Nakano, K K, Bang, A G AG and Fletterick, R J RJ..  (1989)  Human muscle glycogen synthase cDNA sequence: a negatively charged protein with an asymmetric charge distribution..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:2493642]
3. Orho, M M, Nikula-Ijäs, P P, Schalin-Jäntti, C C, Permutt, M A MA and Groop, L C LC..  (1995)  Isolation and characterization of the human muscle glycogen synthase gene..  Diabetes,  [PMID:7657035]
4. Su, X X, Schuler, L L and Shapiro, S S..  (1996)  Cloning and characterization of a glycogen synthase cDNA from human endometrium..  The Journal of steroid biochemistry and molecular biology,  [PMID:9010351]
5. Grimwood, Jane J and 97 more authors..  (2004)  The DNA sequence and biology of human chromosome 19..  Nature,  (1): [PMID:15057824]
6. Skurat, Alexander V AV, Dietrich, Amy D AD and Roach, Peter J PJ..  (2006)  Interaction between glycogenin and glycogen synthase..  Archives of biochemistry and biophysics,  (1): [PMID:17055998]
7. Kollberg, Gittan G and 7 more authors..  (2007)  Cardiomyopathy and exercise intolerance in muscle glycogen storage disease 0..  The New England journal of medicine,  (11): [PMID:17928598]
8. Dephoure, Noah N and 6 more authors..  (2008)  A quantitative atlas of mitotic phosphorylation..  Proceedings of the National Academy of Sciences of the United States of America,  (5): [PMID:18669648]
9. Daub, Henrik H and 9 more authors..  (2008)  Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle..  Molecular cell,  (8): [PMID:18691976]
10. Mayya, Viveka V and 7 more authors..  (2009)  Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions..  Science signaling,  (18): [PMID:19690332]
11. Rigbolt, Kristoffer T G KT and 9 more authors..  (2011)  System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation..  Science signaling,  (15): [PMID:21406692]
12. Kelsall, Ian R IR, Voss, Martin M, Munro, Shonagh S, Cuthbertson, Daniel J R DJ and Cohen, Patricia T W PT..  (2011)  R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals..  Journal of neurochemistry,  [PMID:21668450]
13. Zhou, Houjiang H and 6 more authors..  (2013)  Toward a comprehensive characterization of a human cancer cell phosphoproteome..  Journal of proteome research,  (4): [PMID:23186163]
14. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge.  (2011-01-29)  Initial characterization of the human central proteome..  BMC systems biology,  (17-17).  [PMID:21269460]

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