Eph receptor A2 Antibody

COA

级别和纯度:

无载体

已验证

ExactAb™

1.0 mg/mL

应用:

IF/ICC
IHC
WB

宿主种属: 兔(Rabbit)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse), 猴(Monkey), 鸡(Chicken)
亚型: Rabbit IgG
偶联: Unconjugated

有货

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货号 (SKU)	包装规格	是否现货
Ab101886-10μl	10μl	现货
Ab101886-50μl	50μL	现货
Ab101886-100μl	100μL	期货
Ab101886-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: EPHA2

Accession#:P29317 (4) Eph receptor A2 (2) EphA2 (4) Gene ID:1969 (4)

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基本信息

产品名称	Eph receptor A2 Antibody
别名	Eph receptor A2多克隆抗体 \| 内皮细胞受体蛋白酪氨酸激酶抗体
英文别名	ARCC2 \| EC 2.7.10 \| EC 2.7.10.1 \| Eck \| ECKepithelial cell receptor protein tyrosine kinase \| EPH receptor A2 \| EphA2 \| ephrin type-A receptor 2 \| Epithelial cell kinase \| Myk2 \| Sek2 \| soluble EPHA2 variant 1 \| Tyrosine-protein kinase receptor ECK \| ARCC
规格或纯度	ExactAb™, 已验证, 无载体, 1.0 mg/mL
生化机理	ephrin-A 家族成员的受体。与 ephrin-A1、-A3、-A4 和 -A5 结合。在血管生成和肿瘤新生血管中发挥重要作用。磷酸化的 EPHA2 对 VAV2、VAV3 和 PI3 激酶 p85 亚基的招募对 EFNA1 诱导的 RAC1 GTPase 激活以及血管内皮细胞迁移和组装至关重要（相似）。以 p53/TP53 依赖性和 caspase-8 依赖性方式诱导细胞凋亡。
宿主种属	兔(Rabbit)
特异性	EPHA2
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse),猴(Monkey),鸡(Chicken)
免疫原	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPHA2.
阳性对照	WB: HEK293, HeLa and C6 cell lysates. IHC: Human colorectal cancer tissue. IF/ICC: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	多克隆抗体
Format	Whole IgG
SDS-PAGE	150 kDa
纯化方法	Immunogen affinity purified
物理外观	Liquid
储存缓冲液	0.42% Potassium phosphate, 0.87% Sodium chloride, 30% Glycerol, 0.01% Sodium azide, pH7.3
防腐剂	0.01% Sodium azide
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	4℃ 短期储存（1-2 周）。-20℃长期保存（12 个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型	抗体

关联靶点（人）

EPHA2 Tclin 肝素 A 型受体 2(Ephrin type-A receptor 2) (0 活性数据)

点击查看靶蛋白信息： EPHA2

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

EPHA2 Tclin Ephrin type-A receptor 2 (3499 活性数据)

点击查看靶蛋白信息： EPHA2

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

EPHA2 Tclin Ephrin receptor (0 活性数据)

点击查看靶蛋白信息： EPHA2

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Western Blot: Eph receptor A2 Antibody (Ab101886)

Eph receptor A2 Antibody (Ab101886) - Western Blot
All lanes: Eph receptor A2 Antibody (Ab101886) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 109 kDa
Observed band size: 117 kDa
Exposure time: 15.2 s

Western Blot: Eph receptor A2 Antibody (Ab101886)

IHC: Eph receptor A2 Antibody (Ab101886)

Eph receptor A2 Antibody (Ab101886) - IHC
Immunohistochemical analysis of EPHA2 staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Eph receptor A2 Antibody (Ab101886) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: Eph receptor A2 Antibody (Ab101886)

Eph receptor A2 Antibody (Ab101886) - IF/ICC
Immunofluorescent analysis of EPHA2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X - 100 in TBS for 5 - 10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the Eph receptor A2 Antibody (Ab101886) in 3% BSA - PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594 - conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).