货号 (SKU)

包装规格

是否现货

价格

数量

Ab099620-10μl

10μl

现货

Ab099620-50μl

50μL

现货

Ab099620-100μl

100μL

现货

Ab099620-1ml

1ml

期货

基本信息

产品名称	Density Regulated Protein Mouse mAb
别名	密度调节蛋白抗体 \| 密度调节蛋白单克隆抗体 \| Density Regulated Protein抗体 \| Density Regulated Protein单克隆抗体 \| DENR抗体 \| DENR单克隆抗体
英文别名	denr antibody \| DENR_HUMAN antibody \| Density regulated protein antibody \| Density-regulated protein antibody \| DRP antibody \| DRP1 antibody \| DRP1 protein antibody \| Protein DRP1 antibody \| SMAP-3 antibody \| SMAP3 antibody \| Smooth muscle cell associated
规格或纯度	ExactAb™, 已验证, 无载体, 0.5 mg/mL
生化机理	翻译调节因子与 MCTS1 形成复合物，促进翻译再启动。翻译再启动是指在上游调控 ORF（uORF）翻译终止后，小核糖体亚基仍附着在 mRNA 上，并在同一 mRNA 分子上继续扫描，以启动下游 ORF（通常是主 ORF（mORF））的翻译。MCTS1/DENR 复合物对翻译重启所必需的两种关联机制至关重要。首先，在核糖体循环过程中，脱乙酰 tRNA 与终止后的 40S 核糖体复合物分离。其次，以不依赖于 EIF2 的方式将氨基酰化的启动子 tRNA 招募到 40S 核糖体的 P 位点，以进行新一轮翻译。这种调控机制控制着 150 多个基因的翻译，这些基因的翻译重启依赖于 MCTS1/DENR 复合物。
宿主种属	小鼠(Mouse)
特异性	Density Regulated Protein
种属反应性	人(Human)
免疫原	Recombinant protein of human Density Regulated Protein.
阳性对照	WB: A431, HeLa, HEK293 and Jurkat cell lysates. IHC: Human skeletal muscle tissue. IF/ICC: HeLa cells. Flow Cytometry: HeLa cells.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
亚型	Mouse IgG2b
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, 30% Glycerol, 0.01% Sodium azide, pH 7.3.
防腐剂	0.01% Sodium azide
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	短期储存2-8℃（1-2周）；长期储存-20℃（12个月）；收货后建议分装，避免反复冻融。
分子类型	抗体

关联靶点（人）

DENR Tbio 密度调节蛋白(Density-regulated protein) (0 活性数据)

点击查看靶蛋白信息： DENR

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: Density Regulated Protein Mouse mAb (Ab099620)

Density Regulated Protein Mouse mAb (Ab099620) - Western Blot
All lanes: Density Regulated Protein Mouse mAb (Ab099620) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 29 kDa
Exposure time: 9.7 s

Western Blot: Density Regulated Protein Mouse mAb (Ab099620)

IHC: Density Regulated Protein Mouse mAb (Ab099620)

Density Regulated Protein Mouse mAb (Ab099620) - IHC
Immunohistochemical analysis of DENR staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Density Regulated Protein Mouse mAb (Ab099620) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC: Density Regulated Protein Mouse mAb (Ab099620)

Density Regulated Protein Mouse mAb (Ab099620) - IF/ICC
Immunofluorescent analysis of DENR staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Density Regulated Protein Mouse mAb (Ab099620) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow Cytometry: Density Regulated Protein Mouse mAb (Ab099620)

Density Regulated Protein Mouse mAb (Ab099620) - Flow Cytometry
Flow cytometric analysis of HeLa cells using Density Regulated Protein Mouse mAb (Ab099620). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by Density Regulated Protein Mouse mAb (Ab099620) at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

应用

应用名称	稀释比例
IHC	1/50-1/200
IF/ICC	1/10 - 1/50
Flow Cytometry	1/10-1/50
WB	1/500-1/1000

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找到5个结果

批号(Lot Number)	证书类型	货号
ZJ25F0623349	分析证书	Ab099620
ZJ25F0623350	分析证书	Ab099620
ZJ25F0623351	分析证书	Ab099620
ZJ25F0117053	分析证书	Ab099620
ZJ25F0117052	分析证书	Ab099620

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