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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab095961-10μl |
10μl |
现货 ![]() |
| |
| Ab095961-50μl |
50μL |
现货 ![]() |
| |
| Ab095961-100μl |
100μL |
现货 ![]() |
| |
| Ab095961-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | CEBPA Mouse mAb |
|---|---|
| 别名 | CEBPA 小鼠单克隆抗体 | CEBPA 小鼠单抗 |
| 英文别名 | C/EBP alpha |
| 规格或纯度 | ExactAb™, Validated, Carrier Free, 1.0mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | CEBPA |
| 种属反应性 | 人(Human) |
| 免疫原 | Purified recombinant fragment of human CEBPA expressed in E. Coli. |
| 阳性对照 | WB: THP-1 cell lysate, Flow: HeLa cells. |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | 4E10 |
| Format | Whole IgG |
| 亚型 | IgG1 |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | Purified antibody in PBS with 0.05% sodium azide |
| 防腐剂 | 0.05% sodium azide |
| 浓度 | 1.0mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 4°C 下运输。在 +4°C 下短期储存(1-2 周)。收货后建议分装。在 -20°C 下保存。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
CEBPA Mouse mAb (Ab095961) - Western Blot
All lanes: CEBPA Mouse mAb (Ab095961) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution
Predicted band size: 42&26 kDa
Observed band size: 42&26 kDa
CEBPA Mouse mAb (Ab095961) - Flow Cytometry
Flow cytometric analysis of HeLa cells using CEBPA Mouse mAb (Ab095961) at 1/200 dilution (green) and negative control (red).
| 应用名称 | 稀释比例 |
|---|---|
| WB | 1/500-1/2000 |
| Flow Cytometry |
1/200-1/400
|
| ELISA |
1/10000
|
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab095961 | |
| 分析证书 | Ab095961 | |
| 分析证书 | Ab095961 |
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Antonson, P P and Xanthopoulos, K G KG.. (1995) Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha).. Biochemical and biophysical research communications, (4): [PMID:7575576] |
| 3. Choi, B H BH, Park, G T GT and Rho, H M HM.. (1999) Interaction of hepatitis B viral X protein and CCAAT/ enhancer-binding protein alpha synergistically activates the hepatitis B viral enhancer II/pregenomic promoter.. The Journal of biological chemistry, (29): [PMID:9915821] |
| 4. Aho, S S and 7 more authors.. (2000) Ubinuclein, a novel nuclear protein interacting with cellular and viral transcription factors.. The Journal of cell biology, (20): [PMID:10725330] |
| 5. Pabst, T T and 8 more authors.. (2001) Dominant-negative mutations of CEBPA, encoding CCAAT/enhancer binding protein-alpha (C/EBPalpha), in acute myeloid leukemia.. Nature genetics, [PMID:11242107] |
| 6. Snaddon, Jennifer J and 9 more authors.. (2003) Mutations of CEBPA in acute myeloid leukemia FAB types M1 and M2.. Genes, chromosomes & cancer, [PMID:12661007] |
| 7. Müller, Christine C, Calkhoven, Cornelis F CF, Sha, Xiaojing X and Leutz, Achim A.. (2004) The CCAAT enhancer-binding protein alpha (C/EBPalpha) requires a SWI/SNF complex for proliferation arrest.. The Journal of biological chemistry, (20): [PMID:14660596] |
| 8. Grimwood, Jane J and 97 more authors.. (2004) The DNA sequence and biology of human chromosome 19.. Nature, (1): [PMID:15057824] |
| 9. Wu, Frederick Y FY and 5 more authors.. (2004) CCAAT/enhancer binding protein alpha binds to the Epstein-Barr virus (EBV) ZTA protein through oligomeric interactions and contributes to cooperative transcriptional activation of the ZTA promoter through direct binding to the ZII and ZIIIB motifs during induction of the EBV lytic cycle.. Journal of virology, [PMID:15078966] |
| 10. Wang, Guo-Li GL, Iakova, Polina P, Wilde, Margie M, Awad, Samir S and Timchenko, Nikolai A NA.. (2004) Liver tumors escape negative control of proliferation via PI3K/Akt-mediated block of C/EBP alpha growth inhibitory activity.. Genes & development, (15): [PMID:15107404] |
| 11. Smith, Matthew L ML, Cavenagh, Jamie D JD, Lister, T Andrew TA and Fitzgibbon, Jude J.. (2004) Mutation of CEBPA in familial acute myeloid leukemia.. The New England journal of medicine, (2): [PMID:15575056] |
| 12. Müller, Christine C, Bremer, Anna A, Schreiber, Sandra S, Eichwald, Sabrina S and Calkhoven, Cornelis F CF.. (2010) Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size.. The EMBO journal, (3): [PMID:20075868] |
| 13. Zaragoza, Katrin K, Bégay, Valérie V, Schuetz, Anja A, Heinemann, Udo U and Leutz, Achim A.. (2010) Repression of transcriptional activity of C/EBPalpha by E2F-dimerization partner complexes.. Molecular and cellular biology, [PMID:20176812] |
| 14. Dedhia, Priya H PH and 9 more authors.. (2010) Differential ability of Tribbles family members to promote degradation of C/EBPalpha and induce acute myelogenous leukemia.. Blood, (26): [PMID:20410507] |
| 15. Uljon, Sacha and 7 more authors.. (2016) Structural Basis for Substrate Selectivity of the E3 Ligase COP1.. Structure (London, England : 1993), (3): [PMID:27041596] |
| 16. Brunmeir, Reinhard R and 7 more authors.. (2016) Comparative Transcriptomic and Epigenomic Analyses Reveal New Regulators of Murine Brown Adipogenesis.. PLoS genetics, [PMID:27923061] |