CD55 Mouse mAb

COA

级别和纯度:

无载体

已验证

ExactAb™

见COA

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: HI55a
偶联: Unconjugated

有货

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab095018-10μg	10μg	现货
Ab095018-50μg	50μg	现货
Ab095018-100μg	100μg	期货
Ab095018-1mg	1mg	期货

查看相关系列

点击查看靶蛋白信息: CD55

Accession#: P08174 (3) CD55 (3) DAF (1) Gene ID: 1604 (3) 流式细胞术 (1163)

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基本信息

产品名称	CD55 Mouse mAb
别名	衰变加速因子(CD55)抗体 \| CD55抗体 \| CD55小鼠单克隆抗体 \| CD55小鼠单抗
英文别名	CHAPLE \| CROM \| CD55 antigen \| CD55 molecule, decay accelerating factor for complement (Cromer blood group) \| CD55 \| CR \| CRdecay accelerating factor for complement (CD55, Cromer blood group system) \| CROMDAFcomplement decay-accelerating factor \| DAF \| de
规格或纯度	无载体, ExactAb™, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	CD55
种属反应性	人(Human)
阳性对照	Flow: Human peripheral blood lymphocytes.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	HI55a
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein A purified
物理外观	Liquid
储存缓冲液	10mM PBS, 0.1% Sodium azide, pH 7.2
防腐剂	0.1% Sodium azide
浓度	见COA
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	4°C 下运输。4°C 长期保存（36 个月）。收货后建议分装。
分子类型	抗体

关联靶点（人）

CD55 Tbio 补体衰变加速因子(Complement decay-accelerating factor) (0 活性数据)

点击查看靶蛋白信息： CD55

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD55 Mouse mAb (Ab095018)

CD55 Mouse mAb (Ab095018) - Flow Cytometry
Flow Cytometry analysis of HeLa cells labelling CD55 (red) with CD55 Mouse mAb (Ab095018) at 5.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD55 Mouse mAb (Ab095018)

CD55 Mouse mAb (Ab095018) - Flow Cytometry
Flow Cytometry analysis of A549 cells labelling CD55 (red) with CD55 Mouse mAb (Ab095018) at 5.0 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD55 Mouse mAb (Ab095018)

CD55 Mouse mAb (Ab095018) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling CD55 (red) with CD55 Mouse mAb (Ab095018) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD55 Mouse mAb (Ab095018)

CD55 Mouse mAb (Ab095018) - Flow Cytometry
Flow Cytometry analysis of K562 cells labelling CD55 (red) with CD55 Mouse mAb (Ab095018) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	< 0.5µg/100w cells

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0505967	分析证书	Ab095018
ZJ24F0505968	分析证书	Ab095018
ZJ24F0505969	分析证书	Ab095018

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

引用文献

1. Lin HH, Stacey M, Saxby C, Knott V, Chaudhry Y, Evans D, Gordon S, McKnight AJ, Handford P, Lea S. (2001) Molecular analysis of the epidermal growth factor-like short consensus repeat domain-mediated protein-protein interactions: dissection of the CD97-CD55 complex.. J Biol Chem, 276 (26): (24160-9). [PMID:11297558]
2. Koji Mizuhashi,Taro Chaya,Takashi Kanamoto,Yoshihiro Omori,Takahisa Furukawa. (2015-07-25) Obif, a Transmembrane Protein, Is Required for Bone Mineralization and Spermatogenesis in Mice.. PloS one, 10 ((7)): (e0133704-e0133704). [PMID:26207632]
3. Nakano, Y Y and 5 more authors.. (1992) Complete determination of disulfide bonds localized within the short consensus repeat units of decay accelerating factor (CD55 antigen).. Biochimica et biophysica acta, (12): [PMID:1377029]
4. Ewulonu, U K UK, Ravi, L L and Medof, M E ME.. (1991) Characterization of the decay-accelerating factor gene promoter region.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:1711208]
5. Nakano, Y Y and 5 more authors.. (1991) Isolation of two forms of decay-accelerating factor (DAF) from human urine.. Biochimica et biophysica acta, (8): [PMID:1712233]
6. Reid, M E ME and 7 more authors.. (1991) Biochemical studies on red blood cells from a patient with the Inab phenotype (decay-accelerating factor deficiency).. Blood, (15): [PMID:1720702]
7. Moran, P P, Raab, H H, Kohr, W J WJ and Caras, I W IW.. (1991) Glycophospholipid membrane anchor attachment. Molecular analysis of the cleavage/attachment site.. The Journal of biological chemistry, (15): [PMID:1824699]
8. Sugita, Y Y, Negoro, T T, Matsuda, T T, Sakamoto, T T and Tomita, M M.. (1986) Improved method for the isolation and preliminary characterization of human DAF (decay-accelerating factor).. Journal of biochemistry, [PMID:2428813]
9. Caras, I W IW and 5 more authors.. (1987) Cloning of decay-accelerating factor suggests novel use of splicing to generate two proteins.. Nature, [PMID:2433596]
10. Medof, M E ME and 7 more authors.. (1987) Cloning and characterization of cDNAs encoding the complete sequence of decay-accelerating factor of human complement.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2436222]
11. Bergelson, J M JM and 5 more authors.. (1994) Decay-accelerating factor (CD55), a glycosylphosphatidylinositol-anchored complement regulatory protein, is a receptor for several echoviruses.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:7517044]
12. Lublin, D M DM and 8 more authors.. (1994) Molecular basis of reduced or absent expression of decay-accelerating factor in Cromer blood group phenotypes.. Blood, (15): [PMID:7519480]
13. Ward, T T and 5 more authors.. (1994) Decay-accelerating factor CD55 is identified as the receptor for echovirus 7 using CELICS, a rapid immuno-focal cloning method.. The EMBO journal, (1): [PMID:7525274]
14. Shafren, D R DR and 5 more authors.. (1995) Coxsackieviruses B1, B3, and B5 use decay accelerating factor as a receptor for cell attachment.. Journal of virology, [PMID:7538177]
15. Karnauchow, T M TM and 5 more authors.. (1996) The HeLa cell receptor for enterovirus 70 is decay-accelerating factor (CD55).. Journal of virology, [PMID:8764022]
16. Shafren, D R DR, Dorahy, D J DJ, Ingham, R A RA, Burns, G F GF and Barry, R D RD.. (1997) Coxsackievirus A21 binds to decay-accelerating factor but requires intercellular adhesion molecule 1 for cell entry.. Journal of virology, [PMID:9151867]
17. Williams, Pamela P and 7 more authors.. (2003) Mapping CD55 function. The structure of two pathogen-binding domains at 1.7 A.. The Journal of biological chemistry, (21): [PMID:12499389]
18. Uhrinova, Stanislava S and 6 more authors.. (2003) Solution structure of a functionally active fragment of decay-accelerating factor.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:12672958]
19. Storry, Jill R JR and 11 more authors.. (2003) GUTI: a new antigen in the Cromer blood group system.. Transfusion, [PMID:12675719]
20. Lukacik, P P and 16 more authors.. (2004) Complement regulation at the molecular level: the structure of decay-accelerating factor.. Proceedings of the National Academy of Sciences of the United States of America, (3): [PMID:14734808]
21. Osuka, Fumihiko F and 10 more authors.. (2006) Molecular cloning and characterization of novel splicing variants of human decay-accelerating factor.. Genomics, [PMID:16503113]
22. Gregory, S G SG and 178 more authors.. (2006) The DNA sequence and biological annotation of human chromosome 1.. Nature, (18): [PMID:16710414]
23. Hafenstein, Susan S and 6 more authors.. (2007) Interaction of decay-accelerating factor with coxsackievirus B3.. Journal of virology, [PMID:17804498]
24. Ozen, Ahmet A and 32 more authors.. (2017) CD55 Deficiency, Early-Onset Protein-Losing Enteropathy, and Thrombosis.. The New England journal of medicine, (6): [PMID:28657829]
25. Kurolap, Alina A and 21 more authors.. (2017) Loss of CD55 in Eculizumab-Responsive Protein-Losing Enteropathy.. The New England journal of medicine, (6): [PMID:28657861]
26. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

CD55 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器