货号 (SKU)

包装规格

是否现货

价格

数量

Ab006726-10μg

10μg

现货

Ab006726-50μg

50μg

现货

Ab006726-100μg

100μg

期货

Ab006726-1mg

1mg

期货

基本信息

产品名称	CD28 Mouse mAb
别名	CD28单克隆抗体 \| Anti-CD28抗体 \| CD28抗体 \| CD28小鼠单克隆抗体 \| CD28小鼠单抗
英文别名	CD28 antigen (Tp44) \| CD28 antigen \| CD28 molecule \| CD28 \| MGC138290 \| T-cell-specific surface glycoprotein CD28 \| Tp44 \| CD 28 antibody \| CD28 antibody \| CD28 antigen antibody \| CD28 molecule antibody \| CD28_HUMAN antibody \| MGC138290 antibody \| T cell
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 见COA
宿主种属	小鼠(Mouse)
特异性	CD28
种属反应性	人(Human)
免疫原	Human CD28 transfected cell line
阳性对照	Flow: Human peripheral blood lymphocytes.
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
克隆号	CD28.2
Format	Whole IgG
亚型	Mouse IgG1
轻链亚型	kappa
SDS-PAGE	150 kDa
纯化方法	Protein G purified
物理外观	Liquid
储存缓冲液	10mM PBS, pH 7.2-7.4
防腐剂	No
浓度	见COA
储存温度	2-8°C储存
运输条件	冰袋运输
稳定性与储存	4°C 下运输。4°C 长期保存（12 个月）。收货后建议分装。
分子类型	抗体

关联靶点（人）

CD28 Tbio T 细胞特异性表面糖蛋白 CD28(T-cell-specific surface glycoprotein CD28) (0 活性数据)

点击查看靶蛋白信息： CD28

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: CD28 Mouse mAb (Ab006726)

CD28 Mouse mAb (Ab006726) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD28 (red) with CD28 Mouse mAb (Ab006726) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD28 Mouse mAb (Ab006726)

CD28 Mouse mAb (Ab006726) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD28 with CD28 Mouse mAb (Ab006726) conjugated with biotin (right panel) at 2 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: CD28 Mouse mAb (Ab006726)

CD28 Mouse mAb (Ab006726) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD28 (red) with CD28 Mouse mAb (Ab006726) conjugated with biotin at 2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	1μg/100w cells

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0505151	分析证书	Ab006726
ZJ24F0505152	分析证书	Ab006726
ZJ24F0505153	分析证书	Ab006726

技术文档和文章

利用甲醇通透法进行流式细胞术

Categories: 实验方案（Protocols）

探索主题:

流式细胞术流式抗体流式实验甲醇通透细胞分选细胞分型免疫研究细胞内抗原检测

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

Flow Cytometry Using Methanol Permeabilization

Categories: 实验方案（Protocols）

探索主题:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

可替换产品

引用文献

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1): (40-5). [PMID:14702039]
2. Suchard SJ, Davis PM, Kansal S, Stetsko DK, Brosius R, Tamura J, Schneeweis L, Bryson J, Salcedo T, Wang H et al.. (2013) A monovalent anti-human CD28 domain antibody antagonist: preclinical efficacy and safety.. J Immunol, 191 (9): (4599-610). [PMID:24081989]
3. Tomoko Hayashi, Ji-Hun Mo, Xing Gong, Cyprian Rossetto, Angela Jang, Lucinda Beck, Gregory I Elliott, Irina Kufareva, Ruben Abagyan, David H Broide, Jongdae Lee, Eyal Raz,. (2007-11-16) 3-Hydroxyanthranilic acid inhibits PDK1 activation and suppresses experimental asthma by inducing T cell apoptosis.. Proceedings of the National Academy of Sciences of the United States of America, 104 ((47)): ( 18619-18624 ). [PMID:18003900]
4. Lee, K P KP and 5 more authors.. (1990) The genomic organization of the CD28 gene. Implications for the regulation of CD28 mRNA expression and heterogeneity.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2162892]
5. Aruffo, A A and Seed, B B.. (1987) Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2825196]
6. Blotta, M H MH, Marshall, J D JD, DeKruyff, R H RH and Umetsu, D T DT.. (1996) Cross-linking of the CD40 ligand on human CD4+ T lymphocytes generates a costimulatory signal that up-regulates IL-4 synthesis.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:8617933]
7. Ling, V V and 9 more authors.. (2001) Assembly and annotation of human chromosome 2q33 sequence containing the CD28, CTLA4, and ICOS gene cluster: analysis by computational, comparative, and microarray approaches.. Genomics, [PMID:11735222]
8. Deshpande, Manisha M, Venuprasad, K K, Parab, Pradeep B PB, Saha, Bhaskar B and Mitra, Debashis D.. (2002) A novel CD28 mRNA variant and simultaneous presence of various CD28 mRNA isoforms in human T lymphocytes.. Human immunology, [PMID:11916166]
9. Mikolajczak, Sebastian A SA and 5 more authors.. (2004) The modulation of CD40 ligand signaling by transmembrane CD28 splice variant in human T cells.. The Journal of experimental medicine, (5): [PMID:15067037]
10. Brill, Laurence M LM and 5 more authors.. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.. Analytical chemistry, (15): [PMID:15144186]
11. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621]
12. Wollscheid, Bernd B and 7 more authors.. (2009) Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.. Nature biotechnology, [PMID:19349973]
13. Mayya, Viveka V and 7 more authors.. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.. Science signaling, (18): [PMID:19690332]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

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稀释计算器

计算配制储备溶液所需的稀释度。

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复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

CD28 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器

CD28 Mouse mAb

库存信息

库存信息

库存信息

库存信息

基本信息

产品属性

关联靶点（人）

作用机制

图片

应用

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

相关产品

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器