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TNF alpha Mouse mAb - Primary antibody, specific to Human TNF alpha

    Application:
  • Detection Antibody
  • ELISA
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: C08/3E2
  • Conjugation: Unconjugated
In stock
Item Number
Ab215301
Grouped product items
SKU Size
Availability
Price Qty
Ab215301-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$26.90
Ab215301-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$78.90
Ab215301-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$126.90
Ab215301-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$379.90

mAb (C08/3E2); Mouse anti Human TNF alpha; Detection Antibody, ELISA; Unconjugated

Basic Description

Product Name TNF alpha Mouse mAb - Primary antibody, specific to Human TNF alpha
Synonyms APC1 antibody | APC1 protein antibody | Cachectin antibody | DIF antibody | Differentiation inducing factor antibody | Macrophage cytotoxic factor antibody | Tnf antibody | TNF superfamily member 2 antibody | TNF superfamily, member 2 antibody | TNF, macr
Specifications & Purity ExactAb™, Validated, Animal Free, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is i
Host species Mouse
Specificity Human TNF alpha
Immunogen Recombinant protein corresponding to Human TNF alpha
Conjugation Unconjugated
Grade Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human TNF alpha Antibody, clone C08/3E2, could be used for a Detection Antibody in sandwich ELISAs

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Matched Pairs [Ab156547] TNF alpha Mouse mAb, Capture Antibody
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

TNF Tclin Tumor necrosis factor (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Sandwich ELISA - TNF alpha standard curve
Sandwich ELISA detection of Recombinant Human TNF-α Protein (rp156007) using TNF alpha Mouse mAb (Ab156547) as capture antibody at concentration of 1.0 µg/mL. Biotinylated TNF alpha Mouse mAb (Ab215301) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.97 ng/mL - 1000.0 ng/mL

Indirect ELISA - TNF alpha standard curve
Immobilized Recombinant Human TNF-α Protein (rp156007) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with TNF alpha Mouse mAb (Ab156547). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 59.41 ng/mL

Application

Application Dilution info
ELISA

1.0 µg/mL as a Detection antibody in ELISA

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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