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TIMP2 Mouse mAb - Primary antibody, specific to TIMP2, Mouse IgG1

COA COA
    Application:
  • Flow Cytometry
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 1554CT494.262.47
  • Conjugation: Unconjugated
In stock
Item Number
Ab131343
Grouped product items
SKU Size
Availability
 Price Qty
Ab131343-10μl
10μl
2
$66.90
Ab131343-50μl
50μl
1
$199.90
Ab131343-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab131343-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,499.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (1554CT494.262.47); Mouse anti Human TIMP2 Antibody; WB, IHC, ICC, IF, Flow; Unconjugated

View related series
Click to view target information: TIMP2
Accession#:P16035 (1) Flow Cytometry (1158) Gene ID:7077 (1) TIMP2 (1)

Basic Description

Product Name TIMP2 Mouse mAb - Primary antibody, specific to TIMP2, Mouse IgG1
Synonyms CSC 21K antibody | CSC-21K antibody | CSC21K antibody | Metalloproteinase inhibitor 2 antibody | Metalloproteinase inhibitor 2 precursor antibody | TIMP 2 antibody | TIMP metallopeptidase inhibitor 2 antibody | TIMP-2 antibody | TIMP2 antibody | TIMP2_HUM
Specifications & Purity ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Host species Mouse
Specificity TIMP2
Immunogen Recombinant protein of human TIMP2 expressed in E.coli (AA 1-220).
Positive Control WB: HeLa, A431, HUVEC and A549 cell lysates. IHC: Human kidney tissue. ICC/IF: A549 cells. Flow: K562 cells.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human TIMP2 Antibody, Monoclonal (1554CT494.262.47), could be used for WB, IHC, ICC, IF, Flow and so on.
Application
WB: 1/2000
IHC: 1/200
IF: 1/25
Flow: 1/25
Protein Function
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

TIMP2 Tbio Metalloproteinase inhibitor 2 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

TIMP2 Mouse mAb (Ab131343) - Western Blot
All lanes: TIMP2 Mouse mAb (Ab131343) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 21 kDa
Exposure time: 120.0 s

TIMP2 Mouse mAb (Ab131343) - IF/ICC
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung adenocarcinoma epithelial cell line) cells labeling TIMP2 with TIMP2 Mouse mAb (Ab131343) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).

TIMP2 Mouse mAb (Ab131343) - IHC
Immunohistochemical analysis of paraffin-embedded Human kidney section using TIMP2 Mouse mAb (Ab131343). TIMP2 Mouse mAb (Ab131343) was diluted at 1/200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

TIMP2 Mouse mAb (Ab131343) - IHC
TIMP2 Mouse mAb (Ab131343) staining TIMP2 in human kidney sections by Immunohistochemistry. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6.0). Samples were incubated with TIMP2 Mouse mAb (Ab131343) (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

TIMP2 Mouse mAb (Ab131343) - Flow Cytometry
Overlay histogram showing K562 cells stained with TIMP2 Mouse mAb (Ab131343) (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the TIMP2 Mouse mAb (Ab131343) (1/25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Application

Application Dilution info
Flow Cytometry

1/25
WB

1/2000
IHC

1/200

IF/ICC

1/25

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0102591 Certificate of Analysis Jan 25, 2024 Ab131343
ZJ24F0102590 Certificate of Analysis Jan 25, 2024 Ab131343

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