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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab008407-10μg
|
10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$66.90
|
|
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Ab008407-50μg
|
50μg |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$199.90
|
|
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Ab008407-100μg
|
100μg |
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
|
$299.90
|
|
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Ab008407-1mg
|
1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$999.90
|
|
mAb(22G7-5); Mouse anti Human S100B Antibody; Detection or Capture Antibody, ELISA, CLIA, LF, GICA, FIA; Unconjugated
| Product Name | S100B Mouse mAb - Primary antibody, specific to S100b , Mouse IgG1 |
|---|---|
| Synonyms | S100 calcium-binding protein B | S-100 protein beta chain | S-100 protein subunit beta |
| Specifications & Purity | Carrier Free, ExactAb™, Azide Free, Validated, See COA |
| Host species | Mouse |
| Specificity | S100b |
| Immunogen | Recombinant Human S100B Protein, C-His tag |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, Validated |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 25.6 kDa (Light Chain) & 52.1 kDa (Heavy Chain), under reducing conditions; 174.5 kDa, under non-reducing conditions. |
| Purification Method | Affinity chromatography |
| Matched Pairs | [Ab008408] Monoclonal S100B Antibody as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA |
| Shape | Purified polyclonal antibodies Antibody |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 37°C short term(7 days)Store at 4°C short term(30 days). Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 30.3 pg/mL
Range: 0.1 ng/mL - 3.2 ng/mL
Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 18.8 pg/mL
Range: 0.13 ng/mL - 3.1 ng/mL
S100B Mouse mAb (Ab008407) - SDS-PAGE
3 μg/lane of S100B Mouse mAb (Ab008407) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 26.0 kDa (Light Chain) & 54.2 kDa (Heavy Chain) under reducing conditions and 178.7 kDa under non-reducing conditions.
Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA
·Capture antibody: S100B Mouse mAb (Ab008408)
·Detection antibody: S100B Mouse mAb (Ab008407)
| Application | Dilution info |
|---|---|
| CLIA |
Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
|
| FLFIA |
Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Sep 24, 2023 | Ab008407 | |
| Certificate of Analysis | Sep 24, 2023 | Ab008407 |
Starting at $66.90