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S100B Mouse mAb - Primary antibody, specific to S100b , Mouse IgG1

    Application:
  • Capture Antibody
  • CLIA
  • Detection Antibody
  • ELISA
  • FIA
  • GICA
  • LF
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: 22G7-5
  • Conjugation: Unconjugated
In stock
Item Number
Ab008407
Grouped product items
SKU Size
Availability
Price Qty
Ab008407-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab008407-50μg
50μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$199.90
Ab008407-100μg
100μg
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$299.90
Ab008407-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$999.90

mAb(22G7-5); Mouse anti Human S100B Antibody; Detection or Capture Antibody, ELISA, CLIA, LF, GICA, FIA; Unconjugated

Basic Description

Product Name S100B Mouse mAb - Primary antibody, specific to S100b , Mouse IgG1
Synonyms S100 calcium-binding protein B | S-100 protein beta chain | S-100 protein subunit beta
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species Mouse
Specificity S100b
Immunogen Recombinant Human S100B Protein, C-His tag
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, Validated

Product Properties

Isotype Mouse IgG1
SDS-PAGE 25.6 kDa (Light Chain) & 52.1 kDa (Heavy Chain), under reducing conditions; 174.5 kDa, under non-reducing conditions.
Purification Method Affinity chromatography
Matched Pairs [Ab008408] Monoclonal S100B Antibody as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA
Shape Purified polyclonal antibodies Antibody
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 37°C short term(7 days)Store at 4°C short term(30 days). Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

S100B Tchem Protein S100-B (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008408) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008407) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 30.3 pg/mL
Range: 0.1 ng/mL - 3.2 ng/mL

Sandwich ELISA - S100B protein standard curve
S100B protein was serially diluted 3-fold and captured by S100B Mouse mAb (Ab008407) coated on a Clear Polystyrene Microplate (W291037). S100B Mouse mAb (Ab008408) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Sensitivity: 18.8 pg/mL
Range: 0.13 ng/mL - 3.1 ng/mL

S100B Mouse mAb (Ab008407) - SDS-PAGE
3 μg/lane of S100B Mouse mAb (Ab008407) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 26.0 kDa (Light Chain) & 54.2 kDa (Heavy Chain) under reducing conditions and 178.7 kDa under non-reducing conditions.

Relevance of detection results between aladdin Antibody Pair (Ab008408 & Ab008407) and Roche in CLIA
·Capture antibody: S100B Mouse mAb (Ab008408)
·Detection antibody: S100B Mouse mAb (Ab008407)

Application

Application Dilution info
CLIA

Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE

FLFIA

Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F0901143 Certificate of Analysis Sep 24, 2023 Ab008407
ZJ23F0901142 Certificate of Analysis Sep 24, 2023 Ab008407

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