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RFC4 Mouse mAb - Primary antibody, specific to RFC4

    Application:
  • IF/ICC
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Dog,Human,Monkey,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: OTI1A8
  • Conjugation: Unconjugated
In stock
Item Number
Ab125155
Grouped product items
SKU Size
Availability
Price Qty
Ab125155-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab125155-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab125155-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab125155-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,199.90

mAb (OTI1A8); Mouse anti Human RFC4 Antibody; WB, IHC, IF/ICC; Unconjugated

Basic Description

Product Name RFC4 Mouse mAb - Primary antibody, specific to RFC4
Synonyms A1 37 antibody | A1 37 kDa subunit antibody | Activator 1 37 antibody | Activator 1 37 kDa subunit antibody | Activator 1 subunit 4 antibody | Replication factor C 37 antibody | Replication factor C 37 kDa subunit antibody | Replication factor C subunit 4
Specifications & Purity ExactAb™, Validated, High Performance, See COA
Biochemical and Physiological Mechanisms The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA templ
Host species Mouse
Specificity RFC4
Immunogen Recombinant full length protein of human RFC4.
Positive Control WB: Hep G2, HeLa, A549, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human pancreas, pancreas carcinoma, tonsil tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY RFC4.
Conjugation Unconjugated
Grade ExactAb™, High Performance, Validated
Product Description

Mouse anti Human RFC4 Antibody, Monoclonal (OTI1A8), could be used for WB, IHC, IF/ICC and so on.

Product Properties

SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RFC4 Tbio Replication factor C subunit 4 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

RFC4 Mouse mAb (Ab125155) - Western Blot
All lanes: RFC4 Mouse mAb (Ab125155) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 38 kDa
Exposure time: 61.2 s

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155)

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).

RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).

RFC4 Mouse mAb (Ab125155) - IF/ICC
RFC4 Mouse mAb (Ab125155) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.

Application

Application Dilution info
IF/ICC

1/100

IHC

1/150

WB

1/200-1/4000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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