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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab125155-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab125155-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab125155-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
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Ab125155-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,199.90
|
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mAb (OTI1A8); Mouse anti Human RFC4 Antibody; WB, IHC, IF/ICC; Unconjugated
| Product Name | RFC4 Mouse mAb - Primary antibody, specific to RFC4 |
|---|---|
| Synonyms | A1 37 antibody | A1 37 kDa subunit antibody | Activator 1 37 antibody | Activator 1 37 kDa subunit antibody | Activator 1 subunit 4 antibody | Replication factor C 37 antibody | Replication factor C 37 kDa subunit antibody | Replication factor C subunit 4 |
| Specifications & Purity | ExactAb™, Validated, High Performance, See COA |
| Biochemical and Physiological Mechanisms | The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA templ |
| Host species | Mouse |
| Specificity | RFC4 |
| Immunogen | Recombinant full length protein of human RFC4. |
| Positive Control | WB: Hep G2, HeLa, A549, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human pancreas, pancreas carcinoma, tonsil tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY RFC4. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human RFC4 Antibody, Monoclonal (OTI1A8), could be used for WB, IHC, IF/ICC and so on.
|
| SDS-PAGE | 150 kDa |
|---|---|
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | See COA |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
RFC4 Mouse mAb (Ab125155) - Western Blot
All lanes: RFC4 Mouse mAb (Ab125155) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 38 kDa
Exposure time: 61.2 s
RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155)
RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).
RFC4 Mouse mAb (Ab125155) - IHC
Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).
RFC4 Mouse mAb (Ab125155) - IF/ICC
RFC4 Mouse mAb (Ab125155) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.
| Application | Dilution info |
|---|---|
| IF/ICC | 1/100 |
| IHC | 1/150 |
| WB | 1/200-1/4000 |