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RALBP1 Mouse mAb - Primary antibody, specific to RALBP1, Rabbit IgG

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RALBP1 Mouse mAb - Primary antibody, specific to RALBP1, Rabbit IgG

Grade & Purity:

See COA

Application:

IF/ICC
IHC
IP
WB

Host species: Mouse
Reactive species: Human,Mouse,Rat
Isotype: Mouse IgG1
Clone Number: OTI2A6
Conjugation: Unconjugated

In stock

Item Number

Ab124484

Grouped product items
SKU	Size	Availability	Price	Qty
Ab124484-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$59.90
Ab124484-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$189.90
Ab124484-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$309.90
Ab124484-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,999.90

mAb (OTI2A6); Mouse anti Human RALBP1 Antibody; WB, IHC, IF/ICC, IP; Unconjugated

View related series

Click to view target information: RALBP1

Accession#:Q15311 (1) Gene ID:10928 (1) RALBP1 (1)

Basic Description

Product Name	RALBP1 Mouse mAb - Primary antibody, specific to RALBP1, Rabbit IgG
Synonyms	RLIP1 antibody \| 76 kDa Ral-interacting protein antibody \| 76-kDa Ral-interacting protein antibody \| Dinitrophenyl S-glutathione ATPase antibody \| DNP-SG ATPase antibody \| Ral-interacting protein 1 antibody \| Ral-interacting protein 1, 76-KD antibody \| Ra
Specifications & Purity	KO Validation, ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms	Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of
Host species	Mouse
Specificity	RALBP1
Immunogen	Recombinant f ull length protein of human RALBP1.
Positive Control	WB: HeLa, Hep G2, MCF7, HT-29, NIH/3T3 and C6 cell lysates. IHC: Human breast adenocarcinoma, colon adenocarcinoma, kidney, ovary adenocarcinoma, endometrium adenocarcinoma, prostate, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected
Conjugation	Unconjugated
Grade	ExactAb™, KO Validation, Validated
Product Description	Mouse anti Human RALBP1 Antibody, Monoclonal (OTI2A6), could be used for WB, IHC, IF/ICC, IP and so on.

Product Properties

Isotype	Rabbit IgG
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RALBP1 Tbio RalA-binding protein 1 (0 Activities)

Discover Target: RALBP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - Western Blot
All lanes: RALBP1 Mouse mAb (Ab124484) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 76 kDa
Observed band size: 91 kDa
Exposure time: 60.0 s

IHC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of breast tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

IHC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of colon tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

IHC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Kidney tissue within the normal limits using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

IHC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of ovary tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50)

IHC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of endometrium tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

IHC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded prostate tissue within the normal limits using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

IHC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of prostate tissue using RALBP1 Mouse mAb (Ab124484). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

IF/ICC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IF/ICC
RALBP1 Mouse mAb (Ab124484) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1.

IF/ICC: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - IF/ICC
Immunofluorescent staining of Hep G2 cells using RALBP1 Mouse mAb (Ab124484).

KO: RALBP1 Mouse mAb (Ab124484)

RALBP1 Mouse mAb (Ab124484) - KO
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT) and RALBP1-Knockout HeLa cells (KO) were separated by SDS-PAGE and immunoblotted with RALBP1 Mouse mAb (Ab124484) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

Application

Application	Dilution info
WB	1/1000
IF/ICC	1/100
IHC	1/50
IP	4 μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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