Product Name	Recombinant Human CD40 Ligand/TNFSF5 Protein, >98% SDS-PAGE, high purity
Synonyms	CD154 antigen \| CD154 \| CD40 antigen ligand \| CD40 Ligand \| CD40L \| CD40-L \| CD40LG \| CD40LIGM \| gp39 \| hCD40L \| HIGM1 \| T-B cell-activating molecule \| T-BAM \| T-cell antigen Gp39 \| TNF-related activation protein \| TNFSF5 \| TNFSF5IMD3 \| TRAP \| TRAPtumor n
Grade	ActiBioPure™, Azide Free, Bioactive, Carrier Free
Product Description	Purity:>98%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region. The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms. The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable. CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells. The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.
Specifications & Purity	ActiBioPure™, Bioactive, Carrier Free, Azide Free, ≥98%(SDS-PAGE)
Purity	>98% SDS-PAGE
Bioactivity	Testing in progress
Endotoxin Concentration	<1.0 EU/μg
Expression System	E. coli
Amino Acids	108-261 aa
Sequence	MHHHHHHENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
Protein Tag	N-His
Accession #	P29965
Predicted molecular weight	17.7 kDa
SDS-PAGE	16.0 kDa, reducing conditions; 16.0 kDa, non-reducing conditions

Storage and Shipping

Shape	Transparent gelatinous
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C stable up to 1 year. Avoid freeze / thaw cycle.

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SDS-PAGE: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp169583)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp169583) - SDS-PAGE
Recombinant Human CD40 Ligand/TNFSF5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.0 kDa.

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5 results found

Lot Number	Certificate Type	Date	Item
ZJ23F1201884	Certificate of Analysis	Dec 13, 2023	rp169583
ZJ23F1201883	Certificate of Analysis	Dec 13, 2023	rp169583
ZJ23F1201882	Certificate of Analysis	Dec 13, 2023	rp169583
ZJ23F1201881	Certificate of Analysis	Dec 13, 2023	rp169583
ZJ23R1100168	Certificate of Analysis	Nov 24, 2023	rp169583

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Recombinant Human CD40 Ligand/TNFSF5 Protein, >98% SDS-PAGE, high purity

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