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Recombinant Human CD40 Ligand/TNFSF5 Protein

Grade & Purity:

≥95%(SDS-PAGE)

Expression System: E. coli
Accession #: P29965
Protein Tag: N-His
Bioactivity: Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp169583) with the EC50 of 25.02 ng/mL.
Endotoxin Concentration: <1.0 EU/μg

In stock

Item Number

rp189423

Grouped product items
SKU	Size	Availability	Price
rp189423-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$59.90
rp189423-50μg	50μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$139.90
rp189423-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$219.90
rp189423-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$1,299.90

Carrier Free, ≥95% (SDS-PAGE), Active, E. coli, N-His tag, 108-261 aa

View related series

Click to view protein information: CD40LG

Accession#:P29965 (10) CD40 Ligand/TNFSF5 (1) CD40LG (10) Gene ID:959 (10)

Basic Description

Product Name	Recombinant Human CD40 Ligand/TNFSF5 Protein
Synonyms	CD154 \| CD40 antigen ligand \| CD40 Ligand \| CD40L \| CD40-L \| CD40LG \| CD40LIGM \| gp39 \| CD154 antigen \| hCD40L \| HIGM1 \| T-B cell-activating molecule \| T-BAM \| T-cell antigen Gp39 \| TNF-related activation protein \| TNFSF5 \| TNFSF5IMD3 \| TRAP \| TRAPtumor n
Grade	ActiBioPure™, Bioactive, Carrier Free
Product Description	Protein Function: CD40 Ligand, also known as TNFSF, CD154, TRAP, and gp39, is a 34-39 kDa type II transmembrane glycoprotein that belongs to the TNF superfamily. Mature human CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and an 215 aa extracellular region. The extracellular domain of human CD40 Ligand shares 74% and 76% aa sequence identity with mouse and rat CD40 Ligand, respectively. Similar to other TNF superfamily members, CD40 Ligand forms a bioactive homotrimer, both as membrane bound and soluble forms. The 18 kDa soluble form (aa 113-261) arises from proteolytic processing. Mutation and alternative splicing generate additional forms of CD40 Ligand that are often truncated or non-trimerizable. CD40 Ligand is expressed on platelets, as well as on activated T cells and B cells, basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds to CD40, which is expressed on the surface of B cells, dendritic cells, macrophages, monocytes, platelets, endothelial, and epithelial cells. The interaction of CD40 Ligand with CD40 initiates signaling in both CD40 and CD40 Ligand expressing cells. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.
Specifications & Purity	ActiBioPure™, Bioactive, Carrier Free, ≥95%(SDS-PAGE)
Bioactivity	Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp169583) with the EC50 of 25.02 ng/mL.
Endotoxin Concentration	<1.0 EU/μg
Expression System	E. coli
Amino Acids	108-261 aa
Sequence	MHHHHHHENSFEMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
Protein Tag	N-His
Accession #	P29965
Predicted molecular weight	17.7 kDa
SDS-PAGE	15.4 kDa, under reducing conditions; 15.1 kDa, under non-reducing conditions

Storage and Shipping

Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

Binding Activity: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423) - Binding Activity
Immobilized Recombinant Human CD40 (rp144000) at 1.0 μg/mL can bind Human CD40 Ligand/TNFSF5-biotin (rp189423) with the EC50 of 25.02 ng/mL.

Binding Activity: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423)

ELISA: Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423)

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp189423) - ELISA
Immobilized Recombinant Human CD40 Ligand/TNFSF5 protein (rp189423) at 1.0 μg/mL can bind Dapirolizumab (anti-CD40L) (Ab191960) with the EC₅₀ of 28.88 ng/mL.

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Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

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Lot Number	Certificate Type	Date	Item
ZJ24R0700777	Certificate of Analysis	Jul 12, 2024	rp189423

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