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Recombinant Human CD40 Ligand/TNFSF5 Protein, >95% (SDS-PAGE&HPLC), high purity

  • Expression System: E.coli
  • Accession #: P29965
  • Protein Tag: No tag
  • Bioactivity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.
  • Endotoxin Concentration: <1.0 EU/μg
In stock
Item Number
rp152636
Grouped product items
SKU Size
Availability
Price Qty
rp152636-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$59.90
rp152636-50μg
50μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
rp152636-100μg
100μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$229.90
rp152636-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,989.90

Animal Free, >95% (SDS-PAGE&HPLC), Active, E.coli, No tag,113-263 aa

Basic Description

Product Name Recombinant Human CD40 Ligand/TNFSF5 Protein, >95% (SDS-PAGE&HPLC), high purity
Synonyms CD154 antigen | CD154 | CD40 antigen ligand | CD40 Ligand | CD40L | CD40-L | CD40LG | CD40LIGM | gp39 | hCD40L | HIGM1 | T-B cell-activating molecule | T-BAM | T-cell antigen Gp39 | TNF-related activation protein | TNFSF5 | TNFSF5IMD3 | TRAP | TRAPtumor n
Grade ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free
Product Description

Purity
>95% (SDS-PAGE&HPLC)
Endotoxin level
<1.0 EU/μg
Function
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

Specifications & Purity ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE&HPLC)
Purity >95% (SDS-PAGE&HPLC)
Bioactivity Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/ml.
Endotoxin Concentration <1.0 EU/μg
Expression System E.coli
Species Human
Amino Acids 113-263 aa
Sequence MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLWLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL
Protein Tag No tag
Accession # P29965
Predicted molecular weight 16.3 kDa
SDS-PAGE 16.3 kDa

Storage and Shipping

Shape Lyophilized
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-0.3 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution. Upon receipt, it is recommended to aliquot. Avoid freeze/th

Images

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp152636) - Protein Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10 ng/mL.

Recombinant Human CD40 Ligand/TNFSF5 Protein (rp152636) - SDS-PAGE
Recombinant Human CD40 Ligand/TNFSF5 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 16.3 kDa.

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ23F1001351 Certificate of Analysis Oct 19, 2023 rp152636
ZJ23F1001352 Certificate of Analysis Oct 19, 2023 rp152636
ZJ23F1001353 Certificate of Analysis Oct 19, 2023 rp152636

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