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Recombinant Human CCL8/MCP-2 Protein, ＞96%( SDS-PAGE and HPLC), high purityused for Cell Culture,SDS-PAGE,Active standard various applications

Grade & Purity:

≥96%(SDS-PAGE&HPLC)

Expression System: E.coli
Accession #: P80075
Protein Tag: No tag
Bioactivity: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
Endotoxin Concentration: <1.0 EU/μg
Application: Cell Culture,SDS-PAGE,Active standard

In stock

Item Number

rp148607

Grouped product items
SKU	Size	Availability	Price
rp148607-10μg	10μg	7	$69.90
rp148607-50μg	50μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$299.90
rp148607-100μg	100μg	1	$499.90
rp148607-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,999.90

Animal Free, >96%( SDS-PAGE and HPLC), Active, E.coli, No tag, 24-99 aa

View related series

Click to view protein information: CCL8

Accession#:P80075 (2) CCL8 (3) Gene ID:6355 (2) MCP2 (2)

Basic Description

Product Name	Recombinant Human CCL8/MCP-2 Protein, ＞96%( SDS-PAGE and HPLC), high purityused for Cell Culture,SDS-PAGE,Active standard various applications
Synonyms	C-C motif chemokine 8 \| CCL8 \| Chemokine (C-C motif) ligand 8 \| HC14 \| MCP2 \| MCP-2 \| Member 8 (monocyte chemotactic protein 2) \| Monocyte chemoattractant protein 2 \| SCYA10 \| Small-inducible cytokine A8 \| HC14 \| MCP-2 \| Monocyte chemoattractant protein 2
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description	Purity Human CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family. It is encoded by the gene CCL8. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic function for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells. Purity >96% by SDS-PAGE and HPLC analyses. Additional sequence information Full-length mature C-C motif chemokine 8 chain lacking the signal peptide. Function Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Post-translational N-terminal processed form MCP-2(6-76) is produced by proteolytic cleavage after secretion from peripheral blood monocytes. MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥96%(SDS-PAGE&HPLC)
Purity	＞96%( SDS-PAGE and HPLC)
Bioactivity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
Endotoxin Concentration	<1.0 EU/μg
Expression System	E.coli
Species	Human
Amino Acids	24-99 aa
Sequence	QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP
Protein Tag	No tag
Accession #	P80075
Source	Recombinant
Predicted molecular weight	8.9 kDa
SDS-PAGE	8.9 kDa
Molecule Type	Protein

Storage and Shipping

Application	Cell Culture,SDS-PAGE,Active standard
Shape	Lyophilized
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Upon receipt, it is recommended to aliquot. Store at -20°C, Avoid freeze / thaw cycles. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

Protein Bioactivity: Recombinant Human CCL8/MCP-2 Protein (rp148607)

Recombinant Human CCL8/MCP-2 Protein (rp148607)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/mL.

SDS-PAGE: Recombinant Human CCL8/MCP-2 Protein (rp148607)

Recombinant Human CCL8/MCP-2 Protein (rp148607)-SDS-PAGE
Recombinant Human CCL8/MCP-2 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, Showing a single band at 7.8 kDa.

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3 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0600399	Certificate of Analysis	Jun 21, 2023	rp148607
ZJ23F0600400	Certificate of Analysis	Jun 21, 2023	rp148607
ZJ23F0600401	Certificate of Analysis	Jun 21, 2023	rp148607

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