Grouped product items

SKU

Size

Availability

Price

Qty

Ab124480-10μl

10μl

$69.90

Ab124480-50μl

50μl

$189.90

Ab124480-100μl

100μl

Available within 8-12 weeks(?)

$289.90

Ab124480-1ml

1ml

Available within 8-12 weeks(?)

$2,599.90

Basic Description

Product Name	RALB Mouse mAb - Primary antibody, specific to RALB, Mouse IgG2a
Synonyms	V ral simian leukemia viral oncogene homolog B antibody \| GTP binding protein \| RalB \| RAS-like protein B \| ras-related protein Ral-B \| v-ral simian leukemia viral oncogene homolog B (ras related \| GTP bindingprotein) \| 5730472O18Rik antibody \| dRalb anti
Specifications & Purity	ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species	Mouse
Specificity	RALB
Immunogen	Recombinant human RALB (AA 31-200).
Positive Control	WB: Hep G2, HEK293, A431, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue.
Conjugation	Unconjugated
Grade	Carrier Free, ExactAb™, Validated
Product Description	Mouse anti Human RALB Antibody, Monoclonal (K92021_15B6), could be used for WB, IHC and so on. Application: WB: 1/1000 IHC: 1/2000 Protein Function: Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis.

Product Properties

Isotype	Mouse IgG2a
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein A purified
Shape	Liquid
Concentration	1.0 mg/mL
Storage Temp	Store at -80°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

RALB Tbio Ras-related protein Ral-B (0 Activities)

Discover Target: RALB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Western Blot: RALB Mouse mAb (Ab124480)

RALB Mouse mAb (Ab124480) - Western Blot
All lanes: RALB Mouse mAb (Ab124480) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 32.2 s

IHC: RALB Mouse mAb (Ab124480)

RALB Mouse mAb (Ab124480) - IHC
IHC analysis of human testis tissue by RALB Mouse mAb (Ab124480).
IHC was performed using sections of the formalin-fixed paraffin-embedded human testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RALB Mouse mAb (Ab124480) at 0.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in seminiferous ducts are positively stained at the cytoplasm membrane.

Application

Application	Dilution info
WB	1/1000
IHC	1/2000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

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Analytical Chart:

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2 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0505556	Certificate of Analysis	May 14, 2024	Ab124480
ZJ24F0505557	Certificate of Analysis	May 14, 2024	Ab124480

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RALB Mouse mAb - Primary antibody, specific to RALB, Mouse IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

RALB Mouse mAb - Primary antibody, specific to RALB, Mouse IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews