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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab123380-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$59.90
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Ab123380-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$179.90
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Ab123380-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$289.90
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Ab123380-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
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mAb (T26S8); Mouse anti Human Proteasome 20S alpha 5/PSMA5 Antibody; WB, IHC, ICC, IF; Unconjugated
| Product Name | Proteasome 20S alpha 5/PSMA5 Mouse mAb - Primary antibody, specific to Proteasome 20S alpha 5/PSMA5, Mouse IgG1 |
|---|---|
| Synonyms | Multicatalytic endopeptidase complex zeta chain antibody | Proteasome (prosome macropain) subunit alpha type 5 antibody | Proteasome alpha5 subunit antibody | Proteasome component 5 antibody | Proteasome subunit alpha type 5 antibody | Proteasome subunit |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic |
| Host species | Mouse |
| Specificity | Proteasome 20S alpha 5/PSMA5 |
| Immunogen | Recombinant protein of human Proteasome 20S alpha 5/PSMA5. |
| Positive Control | WB: MCF7, HeLa, Hep G2, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. ICC/IF: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human Proteasome 20S alpha 5/PSMA5 Antibody, Monoclonal (T26S8), could be used for WB, IHC, ICC, IF and so on.
|
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) - Western Blot
All lanes: Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 27 kDa
Exposure time: 86.7 s
Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) - IHC
Immunohistochemical analysis of PSMA5 staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) - IF/ICC
Immunofluorescent analysis of PSMA5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Proteasome 20S alpha 5/PSMA5 Mouse mAb (Ab123380) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin-AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| ICC/IF |
1/10-1/50
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| IHC | 1/50-1/200 |
| WB |
1/500-1/1000
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