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PECI/ECI2 Mouse mAb - Primary antibody, specific to ECI2

    Application:
  • IHC
  • IP
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG2a
  • Clone Number: K16256_15A10
  • Conjugation: Unconjugated
In stock
Item Number
Ab121138
Grouped product items
SKU Size
Availability
Price Qty
Ab121138-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$99.90
Ab121138-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$279.90
Ab121138-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$429.90
Ab121138-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,999.90

mAb (K16256_15A10); Mouse anti Human PECI/ECI2 Antibody; WB, IHC, IP; Unconjugated

Basic Description

Product Name PECI/ECI2 Mouse mAb - Primary antibody, specific to ECI2
Synonyms 2-trans-enoyl-CoA isomerase | ACBD2 | acyl Coenzyme A binding domain containing 2 | D2-enoyl-CoA isomerase | D3 | D3 D2 enoyl CoA isomerase | DBI related protein 1 | DBI-related protein 1 | delta(2)-enoyl-CoA isomerase | Delta(3) | Delta(3) delta(2) enoyl
Specifications & Purity Carrier Free, ExactAb™, Validated, 1.0 mg/mL
Biochemical and Physiological Mechanisms Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
Host species Mouse
Specificity ECI2
Immunogen Recombinant protein of Human PECI/ECI2 (AA 41-390)
Positive Control WB: Raji, Jurkat and K562 cell lysates. IHC: Human liver tissue. IP: Raji lysates.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human PECI/ECI2 Antibody, Monoclonal (K16256_15A10), could be used for WB, IHC, IP and so on

Product Properties

Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

ECI2 Tbio Enoyl-CoA delta isomerase 2, mitochondrial (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

PECI/ECI2 Mouse mAb (Ab121138) - Western Blot
All lanes: PECI/ECI2 Mouse mAb (Ab121138) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39, 35 kDa
PECI/ECI2 Mouse mAb (Ab121138) recognizes two isoforms of PECI/ECI2.
Exposure time: 109.1 s

PECI/ECI2 Mouse mAb (Ab121138) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of PECI/ECI2 Mouse mAb (Ab121138) with Raji lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PECI/ECI2 Mouse mAb (Ab121138) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.
Lane 1: Raji lysate
Lane 2: ECI2 immunoprecipitated from Raji lysate by PECI/ECI2 Mouse mAb (Ab121138)
Lane 3: The same as Lane 2 but mouse lgG was used as IgG isotype control antibody
Result: PECI/ECI2 Mouse mAb (Ab121138) can immunoprecipitate ECI2.

PECI/ECI2 Mouse mAb (Ab121138) - IHC
IHC analysis of liver tissue by PECI/ECI2 Mouse mAb (Ab121138).
IHC was performed using sections of the formalin-fixed paraffin-embedded liver tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PECI/ECI2 Mouse mAb (Ab121138) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Hepatocytes are positively stained at the cytoplasm.

Application

Application Dilution info
WB

1/1000

IP

1/100

IHC

1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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