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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab121138-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$99.90
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Ab121138-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$279.90
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Ab121138-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$429.90
|
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Ab121138-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,999.90
|
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mAb (K16256_15A10); Mouse anti Human PECI/ECI2 Antibody; WB, IHC, IP; Unconjugated
| Product Name | PECI/ECI2 Mouse mAb - Primary antibody, specific to ECI2 |
|---|---|
| Synonyms | 2-trans-enoyl-CoA isomerase | ACBD2 | acyl Coenzyme A binding domain containing 2 | D2-enoyl-CoA isomerase | D3 | D3 D2 enoyl CoA isomerase | DBI related protein 1 | DBI-related protein 1 | delta(2)-enoyl-CoA isomerase | Delta(3) | Delta(3) delta(2) enoyl |
| Specifications & Purity | Carrier Free, ExactAb™, Validated, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates. |
| Host species | Mouse |
| Specificity | ECI2 |
| Immunogen | Recombinant protein of Human PECI/ECI2 (AA 41-390) |
| Positive Control | WB: Raji, Jurkat and K562 cell lysates. IHC: Human liver tissue. IP: Raji lysates. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human PECI/ECI2 Antibody, Monoclonal (K16256_15A10), could be used for WB, IHC, IP and so on
|
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
PECI/ECI2 Mouse mAb (Ab121138) - Western Blot
All lanes: PECI/ECI2 Mouse mAb (Ab121138) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 39, 35 kDa
PECI/ECI2 Mouse mAb (Ab121138) recognizes two isoforms of PECI/ECI2.
Exposure time: 109.1 s
PECI/ECI2 Mouse mAb (Ab121138) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of PECI/ECI2 Mouse mAb (Ab121138) with Raji lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. PECI/ECI2 Mouse mAb (Ab121138) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.
Lane 1: Raji lysate
Lane 2: ECI2 immunoprecipitated from Raji lysate by PECI/ECI2 Mouse mAb (Ab121138)
Lane 3: The same as Lane 2 but mouse lgG was used as IgG isotype control antibody
Result: PECI/ECI2 Mouse mAb (Ab121138) can immunoprecipitate ECI2.
PECI/ECI2 Mouse mAb (Ab121138) - IHC
IHC analysis of liver tissue by PECI/ECI2 Mouse mAb (Ab121138).
IHC was performed using sections of the formalin-fixed paraffin-embedded liver tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PECI/ECI2 Mouse mAb (Ab121138) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Hepatocytes are positively stained at the cytoplasm.
| Application | Dilution info |
|---|---|
| WB |
1/1000
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| IP | 1/100 |
| IHC | 1/200
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