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Nuclease S1 from Aspergillus oryzae - ≥100,000-500,000 units/ml, high purity , CAS No.37288-25-8

COA COA
In stock
Item Number
N128634
Grouped product items
SKU Size
Availability
 Price Qty
N128634-10KU
10KU
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$309.90
N128634-50KU
50KU
Available within 4-8 weeks(?)
Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!
$1,099.90
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Cell Cycle (2830)

Basic Description

Product Name Nuclease S1 from Aspergillus oryzae - ≥100,000-500,000 units/ml, high purity , CAS No.37288-25-8
Synonyms Endonuclease S1
Grade EnzymoPure™
Product Description

Product Description
Chromatographically purified. Specific for single-stranded DNA (ssDNA) degradation. Activity on native (ds) DNA undetectable under the assay conditions. A frozen solution in 30mM sodium acetate, pH 4.6, 50mM NaCl, 1mM ZnCl2, and 50% glycerol.
Nuclease S1 isolated from certain Neurospora and Aspergillus species specifically hydrolyzes both terminal and internal phosphodiester bonds of single-stranded DNA and RNA. Nuclease S1 has a molecular weight of approximately 34 kDa and exists as a monomer. The optimum pH range is 4.0-4.6, and it is activated by Zn2+ and/or Ca2+. Inhibitors are EDTA, citrate and high concentrations of SDS.

Product Application
Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA.It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.
Specifications & Purity EnzymoPure™, ≥100,000-500,000 units/ml
Biochemical and Physiological Mechanisms Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5’-phosphomononucleotide and 5’-phosphooligonucleotide end-products. It is used to digest non
CAS 37288-25-8
Enzyme Commission Number 3.1.30.1

Storage and Shipping

Concentration ≥100,000-500,000 units/ml
Storage Temp Store at -20°C
Shipped In Ice chest + Ice pads
Unit definition One Unit hydrolyzes one microgram of denatured calf thymus DNA per minute at 37°C, pH 4.6.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot Number Certificate Type Date Item
D23261286 Certificate of Analysis Feb 22, 2024 N128634
D23261285 Certificate of Analysis Feb 22, 2024 N128634
G2229367 Certificate of Analysis May 10, 2023 N128634
G2229368 Certificate of Analysis May 10, 2023 N128634
I1507106 Certificate of Analysis Apr 14, 2023 N128634

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